Experiment: RCH2 defined with Zinc 0.032 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1062 and Pf6N2E2_1063 are separated by 23 nucleotides Pf6N2E2_1063 and Pf6N2E2_1064 are separated by 159 nucleotides Pf6N2E2_1064 and Pf6N2E2_1065 are separated by 161 nucleotides Pf6N2E2_1065 and Pf6N2E2_1066 are separated by 133 nucleotides
Pf6N2E2_1062 - UvrD/REP helicase family protein, at 1,238,222 to 1,240,708
_1062
Pf6N2E2_1063 - hypothetical protein, at 1,240,732 to 1,240,860
_1063
Pf6N2E2_1064 - hypothetical protein, at 1,241,020 to 1,242,369
_1064
Pf6N2E2_1065 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 1,242,531 to 1,242,968
_1065
Pf6N2E2_1066 - Permease of the drug/metabolite transporter (DMT) superfamily, at 1,243,102 to 1,243,995
_1066
Position (kb)
1241
1242
1243 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1240.076 kb on + strand, within Pf6N2E2_1062 at 1240.076 kb on + strand, within Pf6N2E2_1062 at 1240.076 kb on + strand, within Pf6N2E2_1062 at 1240.077 kb on - strand, within Pf6N2E2_1062 at 1240.077 kb on - strand, within Pf6N2E2_1062 at 1240.077 kb on - strand, within Pf6N2E2_1062 at 1240.114 kb on + strand, within Pf6N2E2_1062 at 1240.115 kb on - strand at 1240.115 kb on - strand, within Pf6N2E2_1062 at 1240.376 kb on + strand, within Pf6N2E2_1062 at 1240.376 kb on + strand, within Pf6N2E2_1062 at 1240.376 kb on + strand, within Pf6N2E2_1062 at 1240.376 kb on + strand, within Pf6N2E2_1062 at 1240.451 kb on + strand, within Pf6N2E2_1062 at 1240.451 kb on + strand, within Pf6N2E2_1062 at 1240.451 kb on + strand, within Pf6N2E2_1062 at 1240.451 kb on + strand, within Pf6N2E2_1062 at 1240.452 kb on - strand, within Pf6N2E2_1062 at 1240.483 kb on - strand at 1240.547 kb on + strand at 1240.547 kb on + strand at 1240.547 kb on + strand at 1240.547 kb on + strand at 1240.547 kb on + strand at 1240.580 kb on + strand at 1240.587 kb on - strand at 1240.587 kb on - strand at 1240.730 kb on - strand at 1240.738 kb on + strand at 1240.738 kb on + strand at 1240.738 kb on + strand at 1240.738 kb on + strand at 1240.738 kb on + strand at 1240.738 kb on + strand at 1240.738 kb on + strand at 1240.739 kb on - strand at 1240.739 kb on - strand at 1240.739 kb on - strand at 1240.739 kb on - strand at 1240.744 kb on + strand at 1240.744 kb on + strand at 1240.745 kb on - strand, within Pf6N2E2_1063 at 1240.947 kb on + strand at 1241.051 kb on + strand at 1241.051 kb on + strand at 1241.263 kb on + strand, within Pf6N2E2_1064 at 1241.263 kb on + strand, within Pf6N2E2_1064 at 1241.263 kb on + strand, within Pf6N2E2_1064 at 1241.263 kb on + strand, within Pf6N2E2_1064 at 1241.264 kb on - strand, within Pf6N2E2_1064 at 1241.264 kb on - strand, within Pf6N2E2_1064 at 1241.264 kb on - strand, within Pf6N2E2_1064 at 1241.277 kb on - strand, within Pf6N2E2_1064 at 1241.277 kb on - strand, within Pf6N2E2_1064 at 1241.284 kb on + strand, within Pf6N2E2_1064 at 1241.285 kb on - strand, within Pf6N2E2_1064 at 1241.285 kb on - strand, within Pf6N2E2_1064 at 1241.285 kb on - strand, within Pf6N2E2_1064 at 1241.348 kb on - strand, within Pf6N2E2_1064 at 1241.368 kb on + strand, within Pf6N2E2_1064 at 1241.391 kb on + strand, within Pf6N2E2_1064 at 1241.468 kb on - strand, within Pf6N2E2_1064 at 1241.468 kb on - strand, within Pf6N2E2_1064 at 1241.468 kb on - strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.483 kb on - strand, within Pf6N2E2_1064 at 1241.483 kb on - strand, within Pf6N2E2_1064 at 1241.483 kb on - strand, within Pf6N2E2_1064 at 1241.483 kb on - strand, within Pf6N2E2_1064 at 1241.483 kb on - strand, within Pf6N2E2_1064 at 1241.484 kb on + strand, within Pf6N2E2_1064 at 1241.492 kb on + strand, within Pf6N2E2_1064 at 1241.500 kb on + strand, within Pf6N2E2_1064 at 1241.500 kb on + strand at 1241.501 kb on - strand, within Pf6N2E2_1064 at 1241.548 kb on + strand, within Pf6N2E2_1064 at 1241.548 kb on + strand, within Pf6N2E2_1064 at 1241.549 kb on - strand, within Pf6N2E2_1064 at 1241.549 kb on - strand, within Pf6N2E2_1064 at 1241.550 kb on + strand, within Pf6N2E2_1064 at 1241.551 kb on - strand, within Pf6N2E2_1064 at 1241.680 kb on + strand, within Pf6N2E2_1064 at 1241.786 kb on - strand, within Pf6N2E2_1064 at 1241.786 kb on - strand, within Pf6N2E2_1064 at 1241.786 kb on - strand, within Pf6N2E2_1064 at 1241.786 kb on - strand, within Pf6N2E2_1064 at 1241.805 kb on + strand, within Pf6N2E2_1064 at 1242.182 kb on - strand, within Pf6N2E2_1064 at 1242.218 kb on - strand, within Pf6N2E2_1064 at 1242.218 kb on - strand, within Pf6N2E2_1064 at 1242.218 kb on - strand, within Pf6N2E2_1064 at 1242.218 kb on - strand, within Pf6N2E2_1064 at 1242.247 kb on + strand at 1242.248 kb on - strand at 1242.248 kb on - strand at 1242.331 kb on - strand at 1242.335 kb on + strand at 1242.336 kb on - strand at 1242.532 kb on + strand at 1242.532 kb on + strand at 1242.533 kb on - strand at 1242.533 kb on - strand at 1242.603 kb on - strand, within Pf6N2E2_1065 at 1242.623 kb on + strand, within Pf6N2E2_1065 at 1242.624 kb on - strand, within Pf6N2E2_1065 at 1242.624 kb on - strand, within Pf6N2E2_1065 at 1242.818 kb on - strand, within Pf6N2E2_1065 at 1243.071 kb on + strand at 1243.072 kb on - strand at 1243.115 kb on + strand at 1243.116 kb on - strand at 1243.116 kb on - strand at 1243.317 kb on - strand, within Pf6N2E2_1066
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Zinc 0.032 mM remove 1,240,076 + Pf6N2E2_1062 0.75 -0.2 1,240,076 + Pf6N2E2_1062 0.75 +1.6 1,240,076 + Pf6N2E2_1062 0.75 -0.5 1,240,077 - Pf6N2E2_1062 0.75 +0.4 1,240,077 - Pf6N2E2_1062 0.75 -0.3 1,240,077 - Pf6N2E2_1062 0.75 -2.8 1,240,114 + Pf6N2E2_1062 0.76 +0.7 1,240,115 - +1.4 1,240,115 - Pf6N2E2_1062 0.76 +1.3 1,240,376 + Pf6N2E2_1062 0.87 -1.0 1,240,376 + Pf6N2E2_1062 0.87 -1.4 1,240,376 + Pf6N2E2_1062 0.87 -0.1 1,240,376 + Pf6N2E2_1062 0.87 +0.2 1,240,451 + Pf6N2E2_1062 0.90 +0.4 1,240,451 + Pf6N2E2_1062 0.90 +0.7 1,240,451 + Pf6N2E2_1062 0.90 +0.9 1,240,451 + Pf6N2E2_1062 0.90 -0.1 1,240,452 - Pf6N2E2_1062 0.90 +0.2 1,240,483 - +1.3 1,240,547 + +0.5 1,240,547 + +2.4 1,240,547 + -0.3 1,240,547 + +0.5 1,240,547 + +0.9 1,240,580 + -0.6 1,240,587 - -0.6 1,240,587 - +0.3 1,240,730 - -0.3 1,240,738 + -1.9 1,240,738 + -0.6 1,240,738 + -1.1 1,240,738 + -2.2 1,240,738 + +0.7 1,240,738 + +0.3 1,240,738 + -0.4 1,240,739 - -0.1 1,240,739 - -1.2 1,240,739 - +0.2 1,240,739 - -0.2 1,240,744 + -1.0 1,240,744 + +1.1 1,240,745 - Pf6N2E2_1063 0.10 +0.7 1,240,947 + -1.3 1,241,051 + -0.2 1,241,051 + -1.9 1,241,263 + Pf6N2E2_1064 0.18 +0.5 1,241,263 + Pf6N2E2_1064 0.18 +0.0 1,241,263 + Pf6N2E2_1064 0.18 -1.2 1,241,263 + Pf6N2E2_1064 0.18 -0.0 1,241,264 - Pf6N2E2_1064 0.18 -0.6 1,241,264 - Pf6N2E2_1064 0.18 +0.1 1,241,264 - Pf6N2E2_1064 0.18 -1.4 1,241,277 - Pf6N2E2_1064 0.19 -1.1 1,241,277 - Pf6N2E2_1064 0.19 -0.8 1,241,284 + Pf6N2E2_1064 0.20 -0.9 1,241,285 - Pf6N2E2_1064 0.20 +0.4 1,241,285 - Pf6N2E2_1064 0.20 -2.8 1,241,285 - Pf6N2E2_1064 0.20 -0.7 1,241,348 - Pf6N2E2_1064 0.24 -1.2 1,241,368 + Pf6N2E2_1064 0.26 +0.8 1,241,391 + Pf6N2E2_1064 0.27 +0.6 1,241,468 - Pf6N2E2_1064 0.33 -0.4 1,241,468 - Pf6N2E2_1064 0.33 +0.4 1,241,468 - Pf6N2E2_1064 0.33 -0.1 1,241,482 + Pf6N2E2_1064 0.34 +1.1 1,241,482 + Pf6N2E2_1064 0.34 +0.8 1,241,482 + Pf6N2E2_1064 0.34 +1.4 1,241,482 + Pf6N2E2_1064 0.34 -0.2 1,241,482 + Pf6N2E2_1064 0.34 +2.2 1,241,482 + Pf6N2E2_1064 0.34 +0.2 1,241,482 + Pf6N2E2_1064 0.34 +0.8 1,241,482 + Pf6N2E2_1064 0.34 -0.9 1,241,483 - Pf6N2E2_1064 0.34 -0.7 1,241,483 - Pf6N2E2_1064 0.34 -0.4 1,241,483 - Pf6N2E2_1064 0.34 +0.1 1,241,483 - Pf6N2E2_1064 0.34 +0.4 1,241,483 - Pf6N2E2_1064 0.34 -1.2 1,241,484 + Pf6N2E2_1064 0.34 -0.9 1,241,492 + Pf6N2E2_1064 0.35 +0.7 1,241,500 + Pf6N2E2_1064 0.36 +0.2 1,241,500 + +0.4 1,241,501 - Pf6N2E2_1064 0.36 +1.4 1,241,548 + Pf6N2E2_1064 0.39 -0.4 1,241,548 + Pf6N2E2_1064 0.39 +1.4 1,241,549 - Pf6N2E2_1064 0.39 -0.6 1,241,549 - Pf6N2E2_1064 0.39 -0.3 1,241,550 + Pf6N2E2_1064 0.39 +0.9 1,241,551 - Pf6N2E2_1064 0.39 +1.4 1,241,680 + Pf6N2E2_1064 0.49 -1.6 1,241,786 - Pf6N2E2_1064 0.57 +1.1 1,241,786 - Pf6N2E2_1064 0.57 -0.1 1,241,786 - Pf6N2E2_1064 0.57 -1.6 1,241,786 - Pf6N2E2_1064 0.57 -2.8 1,241,805 + Pf6N2E2_1064 0.58 -0.4 1,242,182 - Pf6N2E2_1064 0.86 -1.4 1,242,218 - Pf6N2E2_1064 0.89 -0.1 1,242,218 - Pf6N2E2_1064 0.89 -0.9 1,242,218 - Pf6N2E2_1064 0.89 +0.1 1,242,218 - Pf6N2E2_1064 0.89 -1.2 1,242,247 + -0.7 1,242,248 - +2.6 1,242,248 - -0.1 1,242,331 - -1.3 1,242,335 + -0.0 1,242,336 - +0.5 1,242,532 + +1.4 1,242,532 + -0.1 1,242,533 - -0.3 1,242,533 - +0.7 1,242,603 - Pf6N2E2_1065 0.16 +1.1 1,242,623 + Pf6N2E2_1065 0.21 +0.6 1,242,624 - Pf6N2E2_1065 0.21 -0.2 1,242,624 - Pf6N2E2_1065 0.21 -0.8 1,242,818 - Pf6N2E2_1065 0.66 -0.1 1,243,071 + +0.3 1,243,072 - -1.4 1,243,115 + +0.7 1,243,116 - -1.1 1,243,116 - +0.7 1,243,317 - Pf6N2E2_1066 0.24 +0.6
Or see this region's nucleotide sequence