Experiment: RCH2 defined with Zinc 0.016 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_391 and Pf6N2E2_392 are separated by 21 nucleotides Pf6N2E2_392 and Pf6N2E2_393 are separated by 168 nucleotides Pf6N2E2_393 and Pf6N2E2_394 are separated by 137 nucleotides
Pf6N2E2_391 - hypothetical protein, at 459,443 to 460,921
_391
Pf6N2E2_392 - Transcriptional regulator, at 460,943 to 461,851
_392
Pf6N2E2_393 - hypothetical protein, at 462,020 to 462,145
_393
Pf6N2E2_394 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 462,283 to 463,059
_394
Position (kb)
460
461
462 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 460.310 kb on + strand, within Pf6N2E2_391 at 460.310 kb on + strand, within Pf6N2E2_391 at 460.473 kb on - strand, within Pf6N2E2_391 at 460.668 kb on + strand, within Pf6N2E2_391 at 460.669 kb on - strand, within Pf6N2E2_391 at 460.783 kb on + strand at 460.848 kb on + strand at 460.848 kb on + strand at 460.848 kb on + strand at 460.849 kb on - strand at 460.849 kb on - strand at 460.849 kb on - strand at 461.022 kb on + strand at 461.023 kb on - strand at 461.025 kb on + strand at 461.026 kb on - strand at 461.026 kb on - strand at 461.026 kb on - strand at 461.308 kb on + strand, within Pf6N2E2_392 at 461.313 kb on + strand, within Pf6N2E2_392 at 461.313 kb on + strand, within Pf6N2E2_392 at 461.314 kb on - strand, within Pf6N2E2_392 at 461.314 kb on - strand, within Pf6N2E2_392 at 461.326 kb on - strand, within Pf6N2E2_392 at 461.326 kb on - strand, within Pf6N2E2_392 at 461.350 kb on - strand, within Pf6N2E2_392 at 461.383 kb on + strand, within Pf6N2E2_392 at 461.394 kb on - strand, within Pf6N2E2_392 at 461.477 kb on + strand, within Pf6N2E2_392 at 461.478 kb on - strand, within Pf6N2E2_392 at 461.677 kb on + strand, within Pf6N2E2_392 at 461.686 kb on + strand, within Pf6N2E2_392 at 461.834 kb on + strand at 461.842 kb on + strand at 461.842 kb on + strand at 461.916 kb on + strand at 461.917 kb on - strand at 461.917 kb on - strand at 461.917 kb on - strand at 461.990 kb on + strand at 461.990 kb on + strand at 461.990 kb on + strand at 461.990 kb on + strand at 461.991 kb on - strand at 462.019 kb on - strand at 462.196 kb on + strand at 462.261 kb on + strand at 462.261 kb on + strand at 462.262 kb on - strand at 462.262 kb on - strand at 462.307 kb on - strand at 462.353 kb on + strand at 462.353 kb on + strand at 462.354 kb on - strand at 462.390 kb on + strand, within Pf6N2E2_394 at 462.390 kb on + strand, within Pf6N2E2_394 at 462.390 kb on + strand, within Pf6N2E2_394 at 462.648 kb on + strand, within Pf6N2E2_394 at 462.720 kb on + strand, within Pf6N2E2_394 at 462.721 kb on - strand, within Pf6N2E2_394 at 462.721 kb on - strand, within Pf6N2E2_394 at 462.721 kb on - strand, within Pf6N2E2_394 at 462.731 kb on - strand, within Pf6N2E2_394 at 462.760 kb on + strand, within Pf6N2E2_394 at 462.761 kb on - strand, within Pf6N2E2_394
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Zinc 0.016 mM remove 460,310 + Pf6N2E2_391 0.59 -1.0 460,310 + Pf6N2E2_391 0.59 +0.1 460,473 - Pf6N2E2_391 0.70 +0.2 460,668 + Pf6N2E2_391 0.83 -1.0 460,669 - Pf6N2E2_391 0.83 -1.0 460,783 + +1.6 460,848 + -1.0 460,848 + +0.5 460,848 + -0.6 460,849 - -1.2 460,849 - +0.8 460,849 - -2.5 461,022 + -0.1 461,023 - +0.6 461,025 + -0.5 461,026 - -0.1 461,026 - -0.4 461,026 - +0.3 461,308 + Pf6N2E2_392 0.40 -0.8 461,313 + Pf6N2E2_392 0.41 -0.8 461,313 + Pf6N2E2_392 0.41 +0.5 461,314 - Pf6N2E2_392 0.41 -1.2 461,314 - Pf6N2E2_392 0.41 -0.7 461,326 - Pf6N2E2_392 0.42 -1.6 461,326 - Pf6N2E2_392 0.42 +0.6 461,350 - Pf6N2E2_392 0.45 -0.7 461,383 + Pf6N2E2_392 0.48 +0.5 461,394 - Pf6N2E2_392 0.50 -0.8 461,477 + Pf6N2E2_392 0.59 -0.2 461,478 - Pf6N2E2_392 0.59 -1.8 461,677 + Pf6N2E2_392 0.81 -1.2 461,686 + Pf6N2E2_392 0.82 -0.2 461,834 + +0.0 461,842 + -0.3 461,842 + -0.0 461,916 + -1.4 461,917 - -1.0 461,917 - -0.2 461,917 - -0.8 461,990 + +0.2 461,990 + -0.4 461,990 + -0.2 461,990 + +0.4 461,991 - -0.0 462,019 - +0.6 462,196 + -0.1 462,261 + +1.2 462,261 + -0.6 462,262 - +1.0 462,262 - +0.2 462,307 - -0.2 462,353 + +0.5 462,353 + -0.5 462,354 - -0.9 462,390 + Pf6N2E2_394 0.14 -0.1 462,390 + Pf6N2E2_394 0.14 -1.4 462,390 + Pf6N2E2_394 0.14 +0.1 462,648 + Pf6N2E2_394 0.47 +1.1 462,720 + Pf6N2E2_394 0.56 +2.1 462,721 - Pf6N2E2_394 0.56 -0.3 462,721 - Pf6N2E2_394 0.56 -0.1 462,721 - Pf6N2E2_394 0.56 +0.1 462,731 - Pf6N2E2_394 0.58 +0.3 462,760 + Pf6N2E2_394 0.61 -0.0 462,761 - Pf6N2E2_394 0.62 -0.1
Or see this region's nucleotide sequence