Experiment: RCH2 defined with Zinc 0.016 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3644 and Pf6N2E2_3645 are separated by 435 nucleotides Pf6N2E2_3645 and Pf6N2E2_3646 are separated by 193 nucleotides
Pf6N2E2_3644 - Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17), at 4,243,510 to 4,245,399
_3644
Pf6N2E2_3645 - Type I secretion outer membrane protein, TolC precursor, at 4,245,835 to 4,247,274
_3645
Pf6N2E2_3646 - 3-deoxy-D-manno-octulosonic-acid transferase (EC 2.-.-.-), at 4,247,468 to 4,248,718
_3646
Position (kb)
4245
4246
4247
4248 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4244.930 kb on + strand, within Pf6N2E2_3644 at 4244.931 kb on - strand, within Pf6N2E2_3644 at 4244.931 kb on - strand, within Pf6N2E2_3644 at 4244.931 kb on - strand, within Pf6N2E2_3644 at 4244.931 kb on - strand, within Pf6N2E2_3644 at 4244.966 kb on + strand, within Pf6N2E2_3644 at 4244.966 kb on + strand, within Pf6N2E2_3644 at 4244.967 kb on - strand, within Pf6N2E2_3644 at 4245.200 kb on + strand, within Pf6N2E2_3644 at 4245.201 kb on - strand, within Pf6N2E2_3644 at 4245.656 kb on + strand at 4245.656 kb on + strand at 4245.656 kb on + strand at 4245.657 kb on - strand at 4245.657 kb on - strand at 4245.657 kb on - strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.747 kb on - strand at 4245.747 kb on - strand at 4245.747 kb on - strand at 4245.748 kb on + strand at 4245.748 kb on + strand at 4245.748 kb on + strand at 4245.748 kb on + strand at 4245.748 kb on + strand at 4245.749 kb on - strand at 4245.749 kb on - strand at 4245.749 kb on - strand at 4245.749 kb on - strand at 4245.749 kb on - strand at 4245.778 kb on + strand at 4245.779 kb on - strand at 4245.779 kb on - strand at 4245.822 kb on + strand at 4245.822 kb on + strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.940 kb on + strand at 4245.940 kb on + strand at 4245.940 kb on + strand at 4246.126 kb on + strand, within Pf6N2E2_3645 at 4246.408 kb on + strand, within Pf6N2E2_3645 at 4246.409 kb on - strand, within Pf6N2E2_3645 at 4246.409 kb on - strand, within Pf6N2E2_3645 at 4246.495 kb on + strand, within Pf6N2E2_3645 at 4246.495 kb on + strand, within Pf6N2E2_3645 at 4246.496 kb on - strand, within Pf6N2E2_3645 at 4246.573 kb on - strand, within Pf6N2E2_3645 at 4246.599 kb on - strand, within Pf6N2E2_3645 at 4246.612 kb on + strand, within Pf6N2E2_3645 at 4246.613 kb on - strand, within Pf6N2E2_3645 at 4246.613 kb on - strand, within Pf6N2E2_3645 at 4246.635 kb on + strand, within Pf6N2E2_3645 at 4246.636 kb on - strand, within Pf6N2E2_3645 at 4246.691 kb on - strand, within Pf6N2E2_3645 at 4246.691 kb on - strand, within Pf6N2E2_3645 at 4246.794 kb on + strand, within Pf6N2E2_3645 at 4247.065 kb on + strand, within Pf6N2E2_3645 at 4247.065 kb on + strand, within Pf6N2E2_3645 at 4247.066 kb on - strand, within Pf6N2E2_3645 at 4247.066 kb on - strand, within Pf6N2E2_3645 at 4247.066 kb on - strand, within Pf6N2E2_3645 at 4247.066 kb on - strand, within Pf6N2E2_3645 at 4247.066 kb on - strand, within Pf6N2E2_3645 at 4247.083 kb on + strand, within Pf6N2E2_3645 at 4247.083 kb on + strand, within Pf6N2E2_3645 at 4247.083 kb on + strand, within Pf6N2E2_3645 at 4247.083 kb on + strand, within Pf6N2E2_3645 at 4247.084 kb on - strand, within Pf6N2E2_3645 at 4247.084 kb on - strand, within Pf6N2E2_3645 at 4247.084 kb on - strand, within Pf6N2E2_3645 at 4247.104 kb on + strand, within Pf6N2E2_3645 at 4247.104 kb on + strand, within Pf6N2E2_3645 at 4247.104 kb on + strand, within Pf6N2E2_3645 at 4247.104 kb on + strand, within Pf6N2E2_3645 at 4247.104 kb on + strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.179 kb on + strand at 4247.180 kb on - strand at 4247.194 kb on + strand at 4247.194 kb on + strand at 4247.195 kb on - strand at 4247.195 kb on - strand at 4247.195 kb on - strand at 4247.272 kb on + strand at 4247.273 kb on - strand at 4247.277 kb on + strand at 4247.277 kb on + strand at 4247.278 kb on - strand at 4247.278 kb on - strand at 4247.278 kb on - strand at 4247.278 kb on - strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.300 kb on - strand at 4247.348 kb on + strand at 4247.348 kb on + strand at 4247.348 kb on + strand at 4247.411 kb on + strand at 4247.412 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Zinc 0.016 mM remove 4,244,930 + Pf6N2E2_3644 0.75 +0.4 4,244,931 - Pf6N2E2_3644 0.75 +0.8 4,244,931 - Pf6N2E2_3644 0.75 -0.2 4,244,931 - Pf6N2E2_3644 0.75 +1.5 4,244,931 - Pf6N2E2_3644 0.75 +0.2 4,244,966 + Pf6N2E2_3644 0.77 +0.5 4,244,966 + Pf6N2E2_3644 0.77 +0.8 4,244,967 - Pf6N2E2_3644 0.77 -0.1 4,245,200 + Pf6N2E2_3644 0.89 +0.5 4,245,201 - Pf6N2E2_3644 0.89 -0.4 4,245,656 + +0.6 4,245,656 + -1.0 4,245,656 + +0.7 4,245,657 - -1.1 4,245,657 - +0.4 4,245,657 - +1.1 4,245,746 + +1.7 4,245,746 + +1.8 4,245,746 + -1.5 4,245,746 + +0.0 4,245,746 + +0.8 4,245,746 + +0.2 4,245,746 + -0.5 4,245,747 - -2.7 4,245,747 - -3.5 4,245,747 - -1.7 4,245,748 + +0.1 4,245,748 + -0.7 4,245,748 + +0.6 4,245,748 + +0.3 4,245,748 + +0.5 4,245,749 - -2.5 4,245,749 - -2.1 4,245,749 - -0.7 4,245,749 - -0.4 4,245,749 - -2.2 4,245,778 + -0.4 4,245,779 - -1.6 4,245,779 - -3.2 4,245,822 + -1.8 4,245,822 + +1.1 4,245,823 - -0.9 4,245,823 - -2.8 4,245,823 - -2.6 4,245,823 - -3.5 4,245,823 - -2.2 4,245,823 - -1.6 4,245,823 - -1.9 4,245,823 - -4.0 4,245,940 + -3.1 4,245,940 + -3.0 4,245,940 + -3.9 4,246,126 + Pf6N2E2_3645 0.20 -1.5 4,246,408 + Pf6N2E2_3645 0.40 -4.4 4,246,409 - Pf6N2E2_3645 0.40 -2.2 4,246,409 - Pf6N2E2_3645 0.40 -4.3 4,246,495 + Pf6N2E2_3645 0.46 -1.1 4,246,495 + Pf6N2E2_3645 0.46 -1.5 4,246,496 - Pf6N2E2_3645 0.46 -1.5 4,246,573 - Pf6N2E2_3645 0.51 -1.1 4,246,599 - Pf6N2E2_3645 0.53 -2.4 4,246,612 + Pf6N2E2_3645 0.54 -2.5 4,246,613 - Pf6N2E2_3645 0.54 -1.2 4,246,613 - Pf6N2E2_3645 0.54 -3.0 4,246,635 + Pf6N2E2_3645 0.56 -3.1 4,246,636 - Pf6N2E2_3645 0.56 -3.1 4,246,691 - Pf6N2E2_3645 0.59 -2.7 4,246,691 - Pf6N2E2_3645 0.59 -0.8 4,246,794 + Pf6N2E2_3645 0.67 -3.1 4,247,065 + Pf6N2E2_3645 0.85 -2.0 4,247,065 + Pf6N2E2_3645 0.85 -1.6 4,247,066 - Pf6N2E2_3645 0.85 -3.8 4,247,066 - Pf6N2E2_3645 0.85 -2.3 4,247,066 - Pf6N2E2_3645 0.85 -2.1 4,247,066 - Pf6N2E2_3645 0.85 -2.0 4,247,066 - Pf6N2E2_3645 0.85 -1.7 4,247,083 + Pf6N2E2_3645 0.87 -1.4 4,247,083 + Pf6N2E2_3645 0.87 -1.8 4,247,083 + Pf6N2E2_3645 0.87 -3.0 4,247,083 + Pf6N2E2_3645 0.87 +0.7 4,247,084 - Pf6N2E2_3645 0.87 -2.8 4,247,084 - Pf6N2E2_3645 0.87 -3.1 4,247,084 - Pf6N2E2_3645 0.87 -3.3 4,247,104 + Pf6N2E2_3645 0.88 -3.5 4,247,104 + Pf6N2E2_3645 0.88 -0.2 4,247,104 + Pf6N2E2_3645 0.88 -0.6 4,247,104 + Pf6N2E2_3645 0.88 -3.0 4,247,104 + Pf6N2E2_3645 0.88 -3.0 4,247,105 - Pf6N2E2_3645 0.88 -2.0 4,247,105 - Pf6N2E2_3645 0.88 -1.4 4,247,105 - Pf6N2E2_3645 0.88 -2.8 4,247,105 - Pf6N2E2_3645 0.88 -4.1 4,247,105 - Pf6N2E2_3645 0.88 -4.1 4,247,105 - Pf6N2E2_3645 0.88 -3.8 4,247,105 - Pf6N2E2_3645 0.88 -4.1 4,247,179 + -1.9 4,247,180 - -2.5 4,247,194 + -2.6 4,247,194 + -2.7 4,247,195 - -2.6 4,247,195 - -0.6 4,247,195 - +1.7 4,247,272 + +0.5 4,247,273 - -0.2 4,247,277 + +2.4 4,247,277 + -0.4 4,247,278 - -0.4 4,247,278 - +0.1 4,247,278 - -0.1 4,247,278 - -0.5 4,247,285 + -0.5 4,247,285 + +0.9 4,247,285 + -0.1 4,247,285 + -1.0 4,247,285 + -0.8 4,247,285 + -0.0 4,247,285 + -0.7 4,247,285 + -0.6 4,247,285 + -2.1 4,247,286 - +0.5 4,247,286 - -1.6 4,247,286 - +0.4 4,247,286 - -0.6 4,247,286 - +0.2 4,247,286 - +0.2 4,247,286 - -1.8 4,247,300 - +0.0 4,247,348 + +1.2 4,247,348 + +0.4 4,247,348 + +0.2 4,247,411 + -1.2 4,247,412 - -1.1
Or see this region's nucleotide sequence