Experiment: RCH2 defined with Zinc 0.016 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1799 and Pf6N2E2_1800 are separated by 10 nucleotides Pf6N2E2_1800 and Pf6N2E2_1801 are separated by 39 nucleotides Pf6N2E2_1801 and Pf6N2E2_1802 are separated by 150 nucleotides Pf6N2E2_1802 and Pf6N2E2_1803 overlap by 8 nucleotides
Pf6N2E2_1799 - ABC transporter permease protein, at 2,129,047 to 2,129,925
_1799
Pf6N2E2_1800 - putative ABC transporter ATP-binding protein, at 2,129,936 to 2,130,700
_1800
Pf6N2E2_1801 - ABC-type polar amino acid transport system protein, substrate-binding protein, at 2,130,740 to 2,131,564
_1801
Pf6N2E2_1802 - polysaccharide deacetylase, at 2,131,715 to 2,132,563
_1802
Pf6N2E2_1803 - Polysaccharide deacetylase, at 2,132,556 to 2,133,443
_1803
Position (kb)
2130
2131
2132 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2129.774 kb on - strand, within Pf6N2E2_1799 at 2129.774 kb on - strand, within Pf6N2E2_1799 at 2129.791 kb on + strand, within Pf6N2E2_1799 at 2129.792 kb on - strand, within Pf6N2E2_1799 at 2129.972 kb on + strand at 2129.972 kb on + strand at 2129.973 kb on - strand at 2129.973 kb on - strand at 2130.648 kb on + strand at 2130.648 kb on + strand at 2130.648 kb on + strand at 2130.648 kb on + strand at 2130.648 kb on + strand at 2130.698 kb on + strand at 2130.699 kb on - strand at 2130.917 kb on + strand, within Pf6N2E2_1801 at 2130.918 kb on - strand, within Pf6N2E2_1801 at 2130.918 kb on - strand, within Pf6N2E2_1801 at 2130.918 kb on - strand, within Pf6N2E2_1801 at 2131.083 kb on - strand, within Pf6N2E2_1801 at 2131.106 kb on + strand, within Pf6N2E2_1801 at 2131.107 kb on - strand, within Pf6N2E2_1801 at 2131.107 kb on - strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.371 kb on - strand, within Pf6N2E2_1801 at 2131.371 kb on - strand, within Pf6N2E2_1801 at 2131.371 kb on - strand, within Pf6N2E2_1801 at 2131.371 kb on - strand, within Pf6N2E2_1801 at 2131.678 kb on + strand at 2131.678 kb on + strand at 2131.679 kb on - strand at 2131.687 kb on + strand at 2131.688 kb on - strand at 2131.688 kb on - strand at 2131.688 kb on - strand at 2131.751 kb on + strand at 2131.751 kb on + strand at 2131.752 kb on - strand at 2131.838 kb on + strand, within Pf6N2E2_1802 at 2131.945 kb on - strand, within Pf6N2E2_1802 at 2132.176 kb on + strand, within Pf6N2E2_1802 at 2132.177 kb on - strand, within Pf6N2E2_1802 at 2132.181 kb on - strand, within Pf6N2E2_1802 at 2132.214 kb on - strand, within Pf6N2E2_1802 at 2132.282 kb on + strand, within Pf6N2E2_1802 at 2132.298 kb on - strand, within Pf6N2E2_1802 at 2132.298 kb on - strand, within Pf6N2E2_1802 at 2132.403 kb on + strand, within Pf6N2E2_1802 at 2132.502 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Zinc 0.016 mM remove 2,129,774 - Pf6N2E2_1799 0.83 -0.0 2,129,774 - Pf6N2E2_1799 0.83 +0.6 2,129,791 + Pf6N2E2_1799 0.85 -0.8 2,129,792 - Pf6N2E2_1799 0.85 +0.4 2,129,972 + +0.6 2,129,972 + -0.8 2,129,973 - -0.8 2,129,973 - +0.3 2,130,648 + +0.8 2,130,648 + -1.2 2,130,648 + -0.6 2,130,648 + -0.9 2,130,648 + +1.2 2,130,698 + -0.8 2,130,699 - +0.3 2,130,917 + Pf6N2E2_1801 0.21 +0.6 2,130,918 - Pf6N2E2_1801 0.22 +1.0 2,130,918 - Pf6N2E2_1801 0.22 +0.8 2,130,918 - Pf6N2E2_1801 0.22 -0.0 2,131,083 - Pf6N2E2_1801 0.42 -1.1 2,131,106 + Pf6N2E2_1801 0.44 +1.6 2,131,107 - Pf6N2E2_1801 0.44 -0.8 2,131,107 - Pf6N2E2_1801 0.44 -0.8 2,131,370 + Pf6N2E2_1801 0.76 -0.3 2,131,370 + Pf6N2E2_1801 0.76 +0.5 2,131,370 + Pf6N2E2_1801 0.76 +0.5 2,131,370 + Pf6N2E2_1801 0.76 -1.3 2,131,370 + Pf6N2E2_1801 0.76 -2.1 2,131,370 + Pf6N2E2_1801 0.76 -3.1 2,131,371 - Pf6N2E2_1801 0.76 -0.8 2,131,371 - Pf6N2E2_1801 0.76 -1.0 2,131,371 - Pf6N2E2_1801 0.76 -0.3 2,131,371 - Pf6N2E2_1801 0.76 -2.5 2,131,678 + -0.4 2,131,678 + -1.1 2,131,679 - -2.3 2,131,687 + -0.4 2,131,688 - +0.4 2,131,688 - +1.0 2,131,688 - -0.2 2,131,751 + +0.1 2,131,751 + -0.0 2,131,752 - +0.2 2,131,838 + Pf6N2E2_1802 0.14 +0.5 2,131,945 - Pf6N2E2_1802 0.27 +0.2 2,132,176 + Pf6N2E2_1802 0.54 -0.3 2,132,177 - Pf6N2E2_1802 0.54 -2.5 2,132,181 - Pf6N2E2_1802 0.55 -0.9 2,132,214 - Pf6N2E2_1802 0.59 -0.2 2,132,282 + Pf6N2E2_1802 0.67 -1.0 2,132,298 - Pf6N2E2_1802 0.69 +0.0 2,132,298 - Pf6N2E2_1802 0.69 -0.6 2,132,403 + Pf6N2E2_1802 0.81 +1.2 2,132,502 + +0.3
Or see this region's nucleotide sequence