Experiment: RCH2 defined with Zinc 0.032 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5831 and Pf6N2E2_5832 are separated by 30 nucleotides Pf6N2E2_5832 and Pf6N2E2_5833 are separated by 93 nucleotides Pf6N2E2_5833 and Pf6N2E2_5834 are separated by 20 nucleotides
Pf6N2E2_5831 - Major facilitator family transporter, at 6,647,116 to 6,648,582
_5831
Pf6N2E2_5832 - Sulfate and thiosulfate binding protein CysP, at 6,648,613 to 6,649,611
_5832
Pf6N2E2_5833 - Potassium voltage-gated channel subfamily KQT; possible potassium channel, VIC family, at 6,649,705 to 6,650,529
_5833
Pf6N2E2_5834 - Eukaryotic-type low-affinity urea transporter, at 6,650,550 to 6,651,527
_5834
Position (kb)
6648
6649
6650 Strain fitness (log2 ratio)
-2
-1
0
1 at 6647.648 kb on + strand, within Pf6N2E2_5831 at 6647.648 kb on + strand, within Pf6N2E2_5831 at 6647.649 kb on - strand, within Pf6N2E2_5831 at 6647.649 kb on - strand, within Pf6N2E2_5831 at 6647.718 kb on - strand, within Pf6N2E2_5831 at 6648.002 kb on - strand, within Pf6N2E2_5831 at 6648.002 kb on - strand, within Pf6N2E2_5831 at 6648.002 kb on - strand, within Pf6N2E2_5831 at 6648.002 kb on - strand, within Pf6N2E2_5831 at 6648.002 kb on - strand, within Pf6N2E2_5831 at 6648.002 kb on - strand, within Pf6N2E2_5831 at 6648.002 kb on - strand, within Pf6N2E2_5831 at 6648.057 kb on + strand, within Pf6N2E2_5831 at 6648.129 kb on - strand, within Pf6N2E2_5831 at 6648.219 kb on - strand, within Pf6N2E2_5831 at 6648.219 kb on - strand, within Pf6N2E2_5831 at 6648.221 kb on + strand, within Pf6N2E2_5831 at 6648.222 kb on - strand, within Pf6N2E2_5831 at 6648.222 kb on - strand, within Pf6N2E2_5831 at 6648.222 kb on - strand, within Pf6N2E2_5831 at 6648.496 kb on + strand at 6648.614 kb on + strand at 6648.626 kb on + strand at 6648.737 kb on + strand, within Pf6N2E2_5832 at 6648.737 kb on + strand, within Pf6N2E2_5832 at 6648.737 kb on + strand, within Pf6N2E2_5832 at 6648.738 kb on - strand, within Pf6N2E2_5832 at 6648.773 kb on + strand, within Pf6N2E2_5832 at 6648.773 kb on + strand, within Pf6N2E2_5832 at 6648.773 kb on + strand, within Pf6N2E2_5832 at 6648.773 kb on + strand, within Pf6N2E2_5832 at 6648.774 kb on - strand, within Pf6N2E2_5832 at 6648.815 kb on + strand, within Pf6N2E2_5832 at 6648.815 kb on + strand, within Pf6N2E2_5832 at 6648.815 kb on + strand, within Pf6N2E2_5832 at 6648.815 kb on + strand, within Pf6N2E2_5832 at 6648.816 kb on - strand, within Pf6N2E2_5832 at 6648.824 kb on + strand, within Pf6N2E2_5832 at 6648.825 kb on - strand, within Pf6N2E2_5832 at 6648.915 kb on - strand, within Pf6N2E2_5832 at 6649.118 kb on + strand, within Pf6N2E2_5832 at 6649.118 kb on + strand, within Pf6N2E2_5832 at 6649.119 kb on - strand, within Pf6N2E2_5832 at 6649.119 kb on - strand, within Pf6N2E2_5832 at 6649.136 kb on + strand, within Pf6N2E2_5832 at 6649.136 kb on + strand, within Pf6N2E2_5832 at 6649.136 kb on + strand, within Pf6N2E2_5832 at 6649.136 kb on + strand, within Pf6N2E2_5832 at 6649.136 kb on + strand, within Pf6N2E2_5832 at 6649.137 kb on - strand, within Pf6N2E2_5832 at 6649.137 kb on - strand, within Pf6N2E2_5832 at 6649.137 kb on - strand, within Pf6N2E2_5832 at 6649.137 kb on - strand, within Pf6N2E2_5832 at 6649.137 kb on - strand, within Pf6N2E2_5832 at 6649.142 kb on + strand, within Pf6N2E2_5832 at 6649.142 kb on + strand, within Pf6N2E2_5832 at 6649.143 kb on - strand, within Pf6N2E2_5832 at 6649.490 kb on + strand, within Pf6N2E2_5832 at 6649.490 kb on + strand, within Pf6N2E2_5832 at 6649.490 kb on + strand, within Pf6N2E2_5832 at 6649.491 kb on - strand, within Pf6N2E2_5832 at 6649.491 kb on - strand, within Pf6N2E2_5832 at 6649.491 kb on - strand, within Pf6N2E2_5832 at 6649.491 kb on - strand, within Pf6N2E2_5832 at 6649.499 kb on + strand, within Pf6N2E2_5832 at 6649.500 kb on - strand, within Pf6N2E2_5832 at 6649.520 kb on + strand at 6649.520 kb on + strand at 6649.520 kb on + strand at 6649.521 kb on - strand at 6649.521 kb on - strand at 6649.627 kb on - strand at 6649.627 kb on - strand at 6649.627 kb on - strand at 6649.649 kb on + strand at 6649.662 kb on - strand at 6649.662 kb on - strand at 6649.676 kb on - strand at 6649.872 kb on - strand, within Pf6N2E2_5833 at 6649.970 kb on + strand, within Pf6N2E2_5833 at 6649.970 kb on + strand, within Pf6N2E2_5833 at 6649.970 kb on + strand, within Pf6N2E2_5833 at 6649.970 kb on + strand, within Pf6N2E2_5833 at 6649.970 kb on + strand, within Pf6N2E2_5833 at 6649.971 kb on - strand, within Pf6N2E2_5833 at 6649.971 kb on - strand, within Pf6N2E2_5833 at 6649.971 kb on - strand, within Pf6N2E2_5833 at 6650.021 kb on + strand, within Pf6N2E2_5833 at 6650.021 kb on + strand, within Pf6N2E2_5833 at 6650.021 kb on + strand, within Pf6N2E2_5833 at 6650.022 kb on - strand, within Pf6N2E2_5833 at 6650.022 kb on - strand, within Pf6N2E2_5833 at 6650.114 kb on + strand, within Pf6N2E2_5833 at 6650.114 kb on + strand, within Pf6N2E2_5833 at 6650.114 kb on + strand, within Pf6N2E2_5833 at 6650.115 kb on - strand, within Pf6N2E2_5833 at 6650.115 kb on - strand, within Pf6N2E2_5833 at 6650.132 kb on + strand, within Pf6N2E2_5833 at 6650.195 kb on + strand, within Pf6N2E2_5833 at 6650.195 kb on + strand, within Pf6N2E2_5833 at 6650.196 kb on - strand, within Pf6N2E2_5833 at 6650.210 kb on + strand, within Pf6N2E2_5833 at 6650.210 kb on + strand, within Pf6N2E2_5833 at 6650.211 kb on - strand, within Pf6N2E2_5833 at 6650.211 kb on - strand, within Pf6N2E2_5833 at 6650.211 kb on - strand, within Pf6N2E2_5833 at 6650.213 kb on + strand, within Pf6N2E2_5833 at 6650.213 kb on + strand, within Pf6N2E2_5833 at 6650.214 kb on - strand, within Pf6N2E2_5833 at 6650.276 kb on + strand, within Pf6N2E2_5833 at 6650.277 kb on - strand, within Pf6N2E2_5833 at 6650.277 kb on - strand, within Pf6N2E2_5833 at 6650.281 kb on + strand, within Pf6N2E2_5833 at 6650.301 kb on - strand, within Pf6N2E2_5833 at 6650.316 kb on - strand, within Pf6N2E2_5833 at 6650.373 kb on - strand, within Pf6N2E2_5833 at 6650.379 kb on + strand, within Pf6N2E2_5833 at 6650.379 kb on + strand, within Pf6N2E2_5833 at 6650.496 kb on - strand at 6650.496 kb on - strand at 6650.496 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Zinc 0.032 mM remove 6,647,648 + Pf6N2E2_5831 0.36 -0.8 6,647,648 + Pf6N2E2_5831 0.36 -1.7 6,647,649 - Pf6N2E2_5831 0.36 -1.2 6,647,649 - Pf6N2E2_5831 0.36 -2.2 6,647,718 - Pf6N2E2_5831 0.41 -1.4 6,648,002 - Pf6N2E2_5831 0.60 -0.3 6,648,002 - Pf6N2E2_5831 0.60 +0.9 6,648,002 - Pf6N2E2_5831 0.60 -2.1 6,648,002 - Pf6N2E2_5831 0.60 -1.4 6,648,002 - Pf6N2E2_5831 0.60 -0.1 6,648,002 - Pf6N2E2_5831 0.60 -0.5 6,648,002 - Pf6N2E2_5831 0.60 -1.5 6,648,057 + Pf6N2E2_5831 0.64 -0.1 6,648,129 - Pf6N2E2_5831 0.69 +0.5 6,648,219 - Pf6N2E2_5831 0.75 +0.1 6,648,219 - Pf6N2E2_5831 0.75 -0.0 6,648,221 + Pf6N2E2_5831 0.75 +0.4 6,648,222 - Pf6N2E2_5831 0.75 -0.8 6,648,222 - Pf6N2E2_5831 0.75 -1.1 6,648,222 - Pf6N2E2_5831 0.75 +0.5 6,648,496 + -0.2 6,648,614 + +0.4 6,648,626 + -1.1 6,648,737 + Pf6N2E2_5832 0.12 -0.8 6,648,737 + Pf6N2E2_5832 0.12 +0.8 6,648,737 + Pf6N2E2_5832 0.12 +1.0 6,648,738 - Pf6N2E2_5832 0.13 +1.0 6,648,773 + Pf6N2E2_5832 0.16 +0.2 6,648,773 + Pf6N2E2_5832 0.16 -0.6 6,648,773 + Pf6N2E2_5832 0.16 -0.2 6,648,773 + Pf6N2E2_5832 0.16 -1.3 6,648,774 - Pf6N2E2_5832 0.16 +0.8 6,648,815 + Pf6N2E2_5832 0.20 +0.2 6,648,815 + Pf6N2E2_5832 0.20 +0.3 6,648,815 + Pf6N2E2_5832 0.20 -0.2 6,648,815 + Pf6N2E2_5832 0.20 +0.2 6,648,816 - Pf6N2E2_5832 0.20 -1.1 6,648,824 + Pf6N2E2_5832 0.21 +0.7 6,648,825 - Pf6N2E2_5832 0.21 -1.3 6,648,915 - Pf6N2E2_5832 0.30 -0.5 6,649,118 + Pf6N2E2_5832 0.51 -1.4 6,649,118 + Pf6N2E2_5832 0.51 +0.5 6,649,119 - Pf6N2E2_5832 0.51 -2.4 6,649,119 - Pf6N2E2_5832 0.51 +0.4 6,649,136 + Pf6N2E2_5832 0.52 -0.6 6,649,136 + Pf6N2E2_5832 0.52 -0.4 6,649,136 + Pf6N2E2_5832 0.52 -0.8 6,649,136 + Pf6N2E2_5832 0.52 +0.7 6,649,136 + Pf6N2E2_5832 0.52 +0.5 6,649,137 - Pf6N2E2_5832 0.52 +1.2 6,649,137 - Pf6N2E2_5832 0.52 -0.1 6,649,137 - Pf6N2E2_5832 0.52 -0.4 6,649,137 - Pf6N2E2_5832 0.52 +0.4 6,649,137 - Pf6N2E2_5832 0.52 +0.4 6,649,142 + Pf6N2E2_5832 0.53 -0.5 6,649,142 + Pf6N2E2_5832 0.53 -1.0 6,649,143 - Pf6N2E2_5832 0.53 +0.2 6,649,490 + Pf6N2E2_5832 0.88 -1.7 6,649,490 + Pf6N2E2_5832 0.88 -1.4 6,649,490 + Pf6N2E2_5832 0.88 -1.5 6,649,491 - Pf6N2E2_5832 0.88 -0.2 6,649,491 - Pf6N2E2_5832 0.88 -0.6 6,649,491 - Pf6N2E2_5832 0.88 -0.9 6,649,491 - Pf6N2E2_5832 0.88 -0.2 6,649,499 + Pf6N2E2_5832 0.89 -1.5 6,649,500 - Pf6N2E2_5832 0.89 +0.4 6,649,520 + +0.1 6,649,520 + +0.2 6,649,520 + -0.2 6,649,521 - +0.1 6,649,521 - -1.8 6,649,627 - -0.1 6,649,627 - -0.2 6,649,627 - +0.6 6,649,649 + -0.7 6,649,662 - -0.1 6,649,662 - +1.3 6,649,676 - +0.0 6,649,872 - Pf6N2E2_5833 0.20 -0.2 6,649,970 + Pf6N2E2_5833 0.32 +0.2 6,649,970 + Pf6N2E2_5833 0.32 -0.3 6,649,970 + Pf6N2E2_5833 0.32 +0.2 6,649,970 + Pf6N2E2_5833 0.32 -0.3 6,649,970 + Pf6N2E2_5833 0.32 -0.3 6,649,971 - Pf6N2E2_5833 0.32 +0.7 6,649,971 - Pf6N2E2_5833 0.32 +0.2 6,649,971 - Pf6N2E2_5833 0.32 +0.8 6,650,021 + Pf6N2E2_5833 0.38 +0.4 6,650,021 + Pf6N2E2_5833 0.38 -0.3 6,650,021 + Pf6N2E2_5833 0.38 -0.8 6,650,022 - Pf6N2E2_5833 0.38 -0.3 6,650,022 - Pf6N2E2_5833 0.38 -0.2 6,650,114 + Pf6N2E2_5833 0.50 -0.4 6,650,114 + Pf6N2E2_5833 0.50 -0.0 6,650,114 + Pf6N2E2_5833 0.50 +0.7 6,650,115 - Pf6N2E2_5833 0.50 -0.1 6,650,115 - Pf6N2E2_5833 0.50 -0.1 6,650,132 + Pf6N2E2_5833 0.52 -0.6 6,650,195 + Pf6N2E2_5833 0.59 +0.0 6,650,195 + Pf6N2E2_5833 0.59 -0.2 6,650,196 - Pf6N2E2_5833 0.60 -2.3 6,650,210 + Pf6N2E2_5833 0.61 +0.4 6,650,210 + Pf6N2E2_5833 0.61 +0.4 6,650,211 - Pf6N2E2_5833 0.61 -0.3 6,650,211 - Pf6N2E2_5833 0.61 -0.5 6,650,211 - Pf6N2E2_5833 0.61 +0.5 6,650,213 + Pf6N2E2_5833 0.62 -0.3 6,650,213 + Pf6N2E2_5833 0.62 -0.4 6,650,214 - Pf6N2E2_5833 0.62 -1.1 6,650,276 + Pf6N2E2_5833 0.69 -1.0 6,650,277 - Pf6N2E2_5833 0.69 -1.0 6,650,277 - Pf6N2E2_5833 0.69 -0.9 6,650,281 + Pf6N2E2_5833 0.70 -0.4 6,650,301 - Pf6N2E2_5833 0.72 +0.4 6,650,316 - Pf6N2E2_5833 0.74 +0.6 6,650,373 - Pf6N2E2_5833 0.81 -0.7 6,650,379 + Pf6N2E2_5833 0.82 -1.1 6,650,379 + Pf6N2E2_5833 0.82 +0.1 6,650,496 - +1.1 6,650,496 - +0.4 6,650,496 - +0.1
Or see this region's nucleotide sequence