Experiment: RCH2 defined with Zinc 0.016 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5318 and Pf6N2E2_5319 are separated by 3 nucleotides Pf6N2E2_5319 and Pf6N2E2_5320 are separated by 0 nucleotides Pf6N2E2_5320 and Pf6N2E2_5321 are separated by 11 nucleotides Pf6N2E2_5321 and Pf6N2E2_5322 are separated by 216 nucleotides Pf6N2E2_5322 and Pf6N2E2_5323 are separated by 186 nucleotides
Pf6N2E2_5318 - Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-), at 6,113,325 to 6,115,355
_5318
Pf6N2E2_5319 - Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-), at 6,115,359 to 6,115,985
_5319
Pf6N2E2_5320 - Cytochrome O ubiquinol oxidase subunit IV (EC 1.10.3.-), at 6,115,986 to 6,116,321
_5320
Pf6N2E2_5321 - Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB, at 6,116,333 to 6,117,220
_5321
Pf6N2E2_5322 - Predicted transcriptional regulator, at 6,117,437 to 6,117,751
_5322
Pf6N2E2_5323 - FIG00959654: hypothetical protein, at 6,117,938 to 6,118,642
_5323
Position (kb)
6116
6117
6118 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6115.353 kb on + strand at 6115.353 kb on + strand at 6115.353 kb on + strand at 6115.354 kb on - strand at 6115.369 kb on + strand at 6115.369 kb on + strand at 6115.370 kb on - strand at 6115.370 kb on - strand at 6115.464 kb on + strand, within Pf6N2E2_5319 at 6115.465 kb on - strand, within Pf6N2E2_5319 at 6115.509 kb on + strand, within Pf6N2E2_5319 at 6115.524 kb on - strand, within Pf6N2E2_5319 at 6115.572 kb on + strand, within Pf6N2E2_5319 at 6115.573 kb on - strand, within Pf6N2E2_5319 at 6115.576 kb on + strand, within Pf6N2E2_5319 at 6115.576 kb on + strand, within Pf6N2E2_5319 at 6115.576 kb on + strand, within Pf6N2E2_5319 at 6115.577 kb on - strand, within Pf6N2E2_5319 at 6115.577 kb on - strand, within Pf6N2E2_5319 at 6115.617 kb on + strand, within Pf6N2E2_5319 at 6115.617 kb on + strand, within Pf6N2E2_5319 at 6115.618 kb on - strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.855 kb on - strand, within Pf6N2E2_5319 at 6115.855 kb on - strand, within Pf6N2E2_5319 at 6115.855 kb on - strand, within Pf6N2E2_5319 at 6115.855 kb on - strand, within Pf6N2E2_5319 at 6115.855 kb on - strand, within Pf6N2E2_5319 at 6115.965 kb on + strand at 6115.965 kb on + strand at 6115.965 kb on + strand at 6115.966 kb on - strand at 6115.966 kb on - strand at 6115.983 kb on + strand at 6115.983 kb on + strand at 6115.983 kb on + strand at 6115.983 kb on + strand at 6115.984 kb on - strand at 6115.984 kb on - strand at 6115.984 kb on - strand at 6116.044 kb on - strand, within Pf6N2E2_5320 at 6116.103 kb on + strand, within Pf6N2E2_5320 at 6116.103 kb on + strand, within Pf6N2E2_5320 at 6116.104 kb on - strand, within Pf6N2E2_5320 at 6116.104 kb on - strand, within Pf6N2E2_5320 at 6116.164 kb on + strand, within Pf6N2E2_5320 at 6116.164 kb on + strand, within Pf6N2E2_5320 at 6116.164 kb on + strand, within Pf6N2E2_5320 at 6116.165 kb on - strand, within Pf6N2E2_5320 at 6116.181 kb on + strand, within Pf6N2E2_5320 at 6116.181 kb on + strand, within Pf6N2E2_5320 at 6116.181 kb on + strand, within Pf6N2E2_5320 at 6116.182 kb on - strand, within Pf6N2E2_5320 at 6116.182 kb on - strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.636 kb on + strand, within Pf6N2E2_5321 at 6116.691 kb on - strand, within Pf6N2E2_5321 at 6116.691 kb on - strand, within Pf6N2E2_5321 at 6116.691 kb on - strand, within Pf6N2E2_5321 at 6116.703 kb on - strand, within Pf6N2E2_5321 at 6116.703 kb on - strand, within Pf6N2E2_5321 at 6116.711 kb on + strand, within Pf6N2E2_5321 at 6116.784 kb on - strand, within Pf6N2E2_5321 at 6116.864 kb on + strand, within Pf6N2E2_5321 at 6116.865 kb on - strand, within Pf6N2E2_5321 at 6116.865 kb on - strand, within Pf6N2E2_5321 at 6116.865 kb on - strand, within Pf6N2E2_5321 at 6116.966 kb on + strand, within Pf6N2E2_5321 at 6116.966 kb on + strand, within Pf6N2E2_5321 at 6116.967 kb on - strand, within Pf6N2E2_5321 at 6116.967 kb on - strand, within Pf6N2E2_5321 at 6117.029 kb on + strand, within Pf6N2E2_5321 at 6117.029 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.060 kb on - strand, within Pf6N2E2_5321 at 6117.060 kb on - strand, within Pf6N2E2_5321 at 6117.060 kb on - strand, within Pf6N2E2_5321 at 6117.060 kb on - strand, within Pf6N2E2_5321 at 6117.060 kb on - strand, within Pf6N2E2_5321 at 6117.068 kb on + strand, within Pf6N2E2_5321 at 6117.068 kb on + strand, within Pf6N2E2_5321 at 6117.068 kb on + strand, within Pf6N2E2_5321 at 6117.068 kb on + strand, within Pf6N2E2_5321 at 6117.086 kb on + strand, within Pf6N2E2_5321 at 6117.209 kb on + strand at 6117.209 kb on + strand at 6117.209 kb on + strand at 6117.209 kb on + strand at 6117.210 kb on - strand at 6117.210 kb on - strand at 6117.218 kb on + strand at 6117.218 kb on + strand at 6117.218 kb on + strand at 6117.219 kb on - strand at 6117.219 kb on - strand at 6117.219 kb on - strand at 6117.219 kb on - strand at 6117.340 kb on + strand at 6117.355 kb on + strand at 6117.377 kb on - strand at 6117.377 kb on - strand at 6117.377 kb on - strand at 6117.377 kb on - strand at 6117.380 kb on + strand at 6117.380 kb on + strand at 6117.406 kb on + strand at 6117.406 kb on + strand at 6117.407 kb on - strand at 6117.431 kb on + strand at 6117.431 kb on + strand at 6117.431 kb on + strand at 6117.431 kb on + strand at 6117.431 kb on + strand at 6117.431 kb on + strand at 6117.432 kb on - strand at 6117.436 kb on + strand at 6117.436 kb on + strand at 6117.436 kb on + strand at 6117.437 kb on - strand at 6117.437 kb on - strand at 6117.511 kb on + strand, within Pf6N2E2_5322 at 6117.526 kb on - strand, within Pf6N2E2_5322 at 6117.574 kb on - strand, within Pf6N2E2_5322 at 6117.595 kb on + strand, within Pf6N2E2_5322 at 6117.595 kb on + strand, within Pf6N2E2_5322 at 6117.595 kb on + strand, within Pf6N2E2_5322 at 6117.595 kb on + strand, within Pf6N2E2_5322 at 6117.595 kb on + strand, within Pf6N2E2_5322 at 6117.595 kb on + strand, within Pf6N2E2_5322 at 6117.595 kb on + strand, within Pf6N2E2_5322 at 6117.595 kb on + strand, within Pf6N2E2_5322 at 6117.595 kb on + strand, within Pf6N2E2_5322 at 6117.595 kb on + strand, within Pf6N2E2_5322 at 6117.595 kb on + strand, within Pf6N2E2_5322 at 6117.595 kb on + strand, within Pf6N2E2_5322 at 6117.596 kb on - strand, within Pf6N2E2_5322 at 6117.596 kb on - strand, within Pf6N2E2_5322 at 6117.596 kb on - strand, within Pf6N2E2_5322 at 6117.596 kb on - strand, within Pf6N2E2_5322 at 6117.596 kb on - strand, within Pf6N2E2_5322 at 6117.914 kb on + strand at 6117.914 kb on + strand at 6117.915 kb on - strand at 6117.915 kb on - strand at 6117.915 kb on - strand at 6117.915 kb on - strand at 6117.939 kb on + strand at 6117.939 kb on + strand at 6117.939 kb on + strand at 6117.940 kb on - strand at 6117.940 kb on - strand at 6118.079 kb on + strand, within Pf6N2E2_5323
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Zinc 0.016 mM remove 6,115,353 + -0.1 6,115,353 + -0.7 6,115,353 + -1.0 6,115,354 - -0.2 6,115,369 + +0.6 6,115,369 + -0.4 6,115,370 - -0.1 6,115,370 - +0.5 6,115,464 + Pf6N2E2_5319 0.17 +0.8 6,115,465 - Pf6N2E2_5319 0.17 +0.5 6,115,509 + Pf6N2E2_5319 0.24 +0.1 6,115,524 - Pf6N2E2_5319 0.26 +0.3 6,115,572 + Pf6N2E2_5319 0.34 -0.4 6,115,573 - Pf6N2E2_5319 0.34 +0.8 6,115,576 + Pf6N2E2_5319 0.35 +0.9 6,115,576 + Pf6N2E2_5319 0.35 +0.1 6,115,576 + Pf6N2E2_5319 0.35 -0.9 6,115,577 - Pf6N2E2_5319 0.35 +0.1 6,115,577 - Pf6N2E2_5319 0.35 +1.0 6,115,617 + Pf6N2E2_5319 0.41 +0.8 6,115,617 + Pf6N2E2_5319 0.41 -0.3 6,115,618 - Pf6N2E2_5319 0.41 +0.5 6,115,854 + Pf6N2E2_5319 0.79 -1.5 6,115,854 + Pf6N2E2_5319 0.79 -0.6 6,115,854 + Pf6N2E2_5319 0.79 +0.3 6,115,854 + Pf6N2E2_5319 0.79 +1.2 6,115,854 + Pf6N2E2_5319 0.79 +0.2 6,115,854 + Pf6N2E2_5319 0.79 +0.0 6,115,854 + Pf6N2E2_5319 0.79 -1.2 6,115,854 + Pf6N2E2_5319 0.79 -0.1 6,115,855 - Pf6N2E2_5319 0.79 +0.4 6,115,855 - Pf6N2E2_5319 0.79 +0.1 6,115,855 - Pf6N2E2_5319 0.79 +0.8 6,115,855 - Pf6N2E2_5319 0.79 +0.5 6,115,855 - Pf6N2E2_5319 0.79 +0.8 6,115,965 + -0.1 6,115,965 + -0.2 6,115,965 + -0.5 6,115,966 - +0.6 6,115,966 - -0.1 6,115,983 + -0.6 6,115,983 + +0.9 6,115,983 + -0.1 6,115,983 + -0.5 6,115,984 - -0.9 6,115,984 - +0.2 6,115,984 - -0.4 6,116,044 - Pf6N2E2_5320 0.17 +0.3 6,116,103 + Pf6N2E2_5320 0.35 +0.1 6,116,103 + Pf6N2E2_5320 0.35 +1.0 6,116,104 - Pf6N2E2_5320 0.35 -1.2 6,116,104 - Pf6N2E2_5320 0.35 -1.0 6,116,164 + Pf6N2E2_5320 0.53 -0.2 6,116,164 + Pf6N2E2_5320 0.53 +0.2 6,116,164 + Pf6N2E2_5320 0.53 +0.3 6,116,165 - Pf6N2E2_5320 0.53 -1.6 6,116,181 + Pf6N2E2_5320 0.58 +0.1 6,116,181 + Pf6N2E2_5320 0.58 -0.4 6,116,181 + Pf6N2E2_5320 0.58 +0.3 6,116,182 - Pf6N2E2_5320 0.58 +0.1 6,116,182 - Pf6N2E2_5320 0.58 +0.0 6,116,216 + Pf6N2E2_5320 0.68 -0.3 6,116,216 + Pf6N2E2_5320 0.68 -0.2 6,116,216 + Pf6N2E2_5320 0.68 +0.7 6,116,216 + Pf6N2E2_5320 0.68 -1.0 6,116,216 + Pf6N2E2_5320 0.68 -0.4 6,116,216 + Pf6N2E2_5320 0.68 +0.2 6,116,216 + Pf6N2E2_5320 0.68 -0.6 6,116,217 - Pf6N2E2_5320 0.69 +0.5 6,116,217 - Pf6N2E2_5320 0.69 +0.0 6,116,217 - Pf6N2E2_5320 0.69 +0.6 6,116,217 - Pf6N2E2_5320 0.69 -0.5 6,116,217 - Pf6N2E2_5320 0.69 +0.1 6,116,217 - Pf6N2E2_5320 0.69 +0.2 6,116,636 + Pf6N2E2_5321 0.34 +0.3 6,116,691 - Pf6N2E2_5321 0.40 -0.8 6,116,691 - Pf6N2E2_5321 0.40 +0.7 6,116,691 - Pf6N2E2_5321 0.40 +0.8 6,116,703 - Pf6N2E2_5321 0.42 +0.7 6,116,703 - Pf6N2E2_5321 0.42 -0.5 6,116,711 + Pf6N2E2_5321 0.43 -0.6 6,116,784 - Pf6N2E2_5321 0.51 +0.1 6,116,864 + Pf6N2E2_5321 0.60 -0.1 6,116,865 - Pf6N2E2_5321 0.60 -3.3 6,116,865 - Pf6N2E2_5321 0.60 -0.3 6,116,865 - Pf6N2E2_5321 0.60 +0.1 6,116,966 + Pf6N2E2_5321 0.71 -0.3 6,116,966 + Pf6N2E2_5321 0.71 -1.3 6,116,967 - Pf6N2E2_5321 0.71 +1.3 6,116,967 - Pf6N2E2_5321 0.71 -0.2 6,117,029 + Pf6N2E2_5321 0.78 -0.4 6,117,029 + Pf6N2E2_5321 0.78 -0.4 6,117,059 + Pf6N2E2_5321 0.82 -0.1 6,117,059 + Pf6N2E2_5321 0.82 +0.7 6,117,059 + Pf6N2E2_5321 0.82 -0.5 6,117,059 + Pf6N2E2_5321 0.82 -0.5 6,117,059 + Pf6N2E2_5321 0.82 -0.8 6,117,059 + Pf6N2E2_5321 0.82 -0.9 6,117,059 + Pf6N2E2_5321 0.82 +0.5 6,117,060 - Pf6N2E2_5321 0.82 +0.1 6,117,060 - Pf6N2E2_5321 0.82 -0.6 6,117,060 - Pf6N2E2_5321 0.82 -0.5 6,117,060 - Pf6N2E2_5321 0.82 -0.4 6,117,060 - Pf6N2E2_5321 0.82 -0.6 6,117,068 + Pf6N2E2_5321 0.83 -0.4 6,117,068 + Pf6N2E2_5321 0.83 +0.6 6,117,068 + Pf6N2E2_5321 0.83 -1.0 6,117,068 + Pf6N2E2_5321 0.83 +0.4 6,117,086 + Pf6N2E2_5321 0.85 +1.0 6,117,209 + -0.1 6,117,209 + -1.6 6,117,209 + -0.0 6,117,209 + -1.6 6,117,210 - +0.1 6,117,210 - +0.0 6,117,218 + -0.1 6,117,218 + -1.7 6,117,218 + +0.8 6,117,219 - +0.9 6,117,219 - +0.9 6,117,219 - +0.6 6,117,219 - -0.4 6,117,340 + +1.1 6,117,355 + +1.0 6,117,377 - -0.7 6,117,377 - +0.1 6,117,377 - -0.0 6,117,377 - +0.8 6,117,380 + -0.5 6,117,380 + -0.9 6,117,406 + +1.0 6,117,406 + +1.0 6,117,407 - +0.4 6,117,431 + +0.2 6,117,431 + -0.2 6,117,431 + +0.5 6,117,431 + -0.6 6,117,431 + +0.2 6,117,431 + +0.6 6,117,432 - +0.1 6,117,436 + +0.3 6,117,436 + +0.8 6,117,436 + -0.7 6,117,437 - +0.3 6,117,437 - +0.2 6,117,511 + Pf6N2E2_5322 0.23 +0.7 6,117,526 - Pf6N2E2_5322 0.28 -1.0 6,117,574 - Pf6N2E2_5322 0.43 +0.2 6,117,595 + Pf6N2E2_5322 0.50 -0.3 6,117,595 + Pf6N2E2_5322 0.50 -0.9 6,117,595 + Pf6N2E2_5322 0.50 +0.1 6,117,595 + Pf6N2E2_5322 0.50 +0.4 6,117,595 + Pf6N2E2_5322 0.50 +0.3 6,117,595 + Pf6N2E2_5322 0.50 +0.1 6,117,595 + Pf6N2E2_5322 0.50 +0.0 6,117,595 + Pf6N2E2_5322 0.50 +0.2 6,117,595 + Pf6N2E2_5322 0.50 +0.4 6,117,595 + Pf6N2E2_5322 0.50 +1.3 6,117,595 + Pf6N2E2_5322 0.50 -0.5 6,117,595 + Pf6N2E2_5322 0.50 +0.2 6,117,596 - Pf6N2E2_5322 0.50 -1.3 6,117,596 - Pf6N2E2_5322 0.50 +1.3 6,117,596 - Pf6N2E2_5322 0.50 +0.0 6,117,596 - Pf6N2E2_5322 0.50 +0.4 6,117,596 - Pf6N2E2_5322 0.50 +0.4 6,117,914 + -0.2 6,117,914 + -1.0 6,117,915 - +0.1 6,117,915 - -0.4 6,117,915 - -0.2 6,117,915 - +0.0 6,117,939 + +0.6 6,117,939 + +2.0 6,117,939 + +0.4 6,117,940 - -0.8 6,117,940 - +0.2 6,118,079 + Pf6N2E2_5323 0.20 +0.6
Or see this region's nucleotide sequence