Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_643 and Pf6N2E2_644 overlap by 4 nucleotides Pf6N2E2_644 and Pf6N2E2_645 are separated by 116 nucleotides Pf6N2E2_645 and Pf6N2E2_646 are separated by 110 nucleotides Pf6N2E2_646 and Pf6N2E2_647 are separated by 147 nucleotides
Pf6N2E2_643 - General secretion pathway protein J, at 733,969 to 734,580
_643
Pf6N2E2_644 - General secretion pathway protein H, at 734,577 to 735,029
_644
Pf6N2E2_645 - General secretion pathway protein C, at 735,146 to 735,589
_645
Pf6N2E2_646 - General secretion pathway protein I, at 735,700 to 736,074
_646
Pf6N2E2_647 - General secretion pathway protein G, at 736,222 to 736,671
_647
Position (kb) 735
736 Strain fitness (log2 ratio) -2
-1
0
1 at 734.834 kb on + strand, within Pf6N2E2_644 at 735.459 kb on + strand, within Pf6N2E2_645 at 736.159 kb on + strand at 736.171 kb on - strand at 736.171 kb on - strand at 736.171 kb on - strand at 736.519 kb on + strand, within Pf6N2E2_647 at 736.559 kb on - strand, within Pf6N2E2_647 at 736.564 kb on + strand, within Pf6N2E2_647 at 736.565 kb on - strand, within Pf6N2E2_647
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 734,834 + Pf6N2E2_644 0.57 +0.5 735,459 + Pf6N2E2_645 0.70 +0.4 736,159 + -2.2 736,171 - +0.0 736,171 - -1.0 736,171 - -0.5 736,519 + Pf6N2E2_647 0.66 +0.3 736,559 - Pf6N2E2_647 0.75 +0.7 736,564 + Pf6N2E2_647 0.76 +0.1 736,565 - Pf6N2E2_647 0.76 -0.5
Or see this region's nucleotide sequence