Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_586 and Pf6N2E2_587 are separated by 147 nucleotides Pf6N2E2_587 and Pf6N2E2_588 are separated by 65 nucleotides Pf6N2E2_588 and Pf6N2E2_589 are separated by 89 nucleotides Pf6N2E2_589 and Pf6N2E2_590 are separated by 201 nucleotides
Pf6N2E2_586 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 663,474 to 664,874
_586
Pf6N2E2_587 - Hydroxyacylglutathione hydrolase (EC 3.1.2.6), at 665,022 to 665,789
_587
Pf6N2E2_588 - FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-), at 665,855 to 666,613
_588
Pf6N2E2_589 - Ribonuclease HI (EC 3.1.26.4), at 666,703 to 667,155
_589
Pf6N2E2_590 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 667,357 to 668,118
_590
Position (kb) 665
666
667 Strain fitness (log2 ratio) -1
0
1
2
3 at 664.933 kb on + strand at 665.004 kb on + strand at 665.023 kb on + strand at 665.023 kb on + strand at 665.024 kb on - strand at 665.030 kb on + strand at 665.030 kb on + strand at 665.031 kb on - strand at 665.031 kb on - strand at 665.282 kb on + strand, within Pf6N2E2_587 at 665.282 kb on + strand, within Pf6N2E2_587 at 665.282 kb on + strand, within Pf6N2E2_587 at 665.282 kb on + strand, within Pf6N2E2_587 at 665.283 kb on - strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.285 kb on - strand, within Pf6N2E2_587 at 665.285 kb on - strand, within Pf6N2E2_587 at 665.299 kb on + strand, within Pf6N2E2_587 at 665.299 kb on + strand, within Pf6N2E2_587 at 665.300 kb on - strand, within Pf6N2E2_587 at 665.310 kb on - strand, within Pf6N2E2_587 at 665.431 kb on + strand, within Pf6N2E2_587 at 665.431 kb on + strand, within Pf6N2E2_587 at 665.431 kb on + strand, within Pf6N2E2_587 at 665.434 kb on + strand, within Pf6N2E2_587 at 665.470 kb on + strand, within Pf6N2E2_587 at 665.470 kb on + strand, within Pf6N2E2_587 at 665.736 kb on + strand at 665.749 kb on + strand at 665.749 kb on + strand at 665.749 kb on + strand at 665.749 kb on + strand at 665.749 kb on + strand at 665.749 kb on + strand at 665.750 kb on - strand at 665.750 kb on - strand at 665.750 kb on - strand at 665.750 kb on - strand at 665.785 kb on - strand at 665.785 kb on - strand at 665.785 kb on - strand at 665.826 kb on + strand at 665.826 kb on + strand at 665.827 kb on - strand at 666.018 kb on - strand, within Pf6N2E2_588 at 666.489 kb on - strand, within Pf6N2E2_588 at 666.611 kb on + strand at 666.694 kb on + strand at 666.694 kb on + strand at 666.716 kb on + strand at 667.156 kb on + strand at 667.156 kb on + strand at 667.156 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 664,933 + +0.6 665,004 + -0.6 665,023 + +0.1 665,023 + +1.3 665,024 - +1.9 665,030 + -0.1 665,030 + +0.4 665,031 - -0.8 665,031 - -1.6 665,282 + Pf6N2E2_587 0.34 +0.1 665,282 + Pf6N2E2_587 0.34 -0.3 665,282 + Pf6N2E2_587 0.34 -0.5 665,282 + Pf6N2E2_587 0.34 +1.6 665,283 - Pf6N2E2_587 0.34 -0.2 665,284 + Pf6N2E2_587 0.34 +0.1 665,284 + Pf6N2E2_587 0.34 -0.6 665,284 + Pf6N2E2_587 0.34 +0.3 665,284 + Pf6N2E2_587 0.34 -0.4 665,284 + Pf6N2E2_587 0.34 -1.0 665,284 + Pf6N2E2_587 0.34 -0.5 665,284 + Pf6N2E2_587 0.34 +0.1 665,284 + Pf6N2E2_587 0.34 +1.2 665,285 - Pf6N2E2_587 0.34 +1.4 665,285 - Pf6N2E2_587 0.34 +0.5 665,299 + Pf6N2E2_587 0.36 -0.6 665,299 + Pf6N2E2_587 0.36 +0.3 665,300 - Pf6N2E2_587 0.36 -0.5 665,310 - Pf6N2E2_587 0.38 -0.4 665,431 + Pf6N2E2_587 0.53 -0.3 665,431 + Pf6N2E2_587 0.53 +1.4 665,431 + Pf6N2E2_587 0.53 +0.0 665,434 + Pf6N2E2_587 0.54 -0.3 665,470 + Pf6N2E2_587 0.58 -0.1 665,470 + Pf6N2E2_587 0.58 +1.0 665,736 + -0.9 665,749 + -1.3 665,749 + +0.3 665,749 + -0.6 665,749 + -0.1 665,749 + -0.2 665,749 + -0.4 665,750 - -0.0 665,750 - -0.0 665,750 - +0.6 665,750 - -0.2 665,785 - -0.5 665,785 - +1.0 665,785 - -0.0 665,826 + +0.8 665,826 + -0.2 665,827 - -0.0 666,018 - Pf6N2E2_588 0.21 -1.2 666,489 - Pf6N2E2_588 0.84 -0.7 666,611 + +0.8 666,694 + -0.1 666,694 + -0.2 666,716 + -0.2 667,156 + +2.8 667,156 + -0.1 667,156 + +0.1
Or see this region's nucleotide sequence