Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_52 and Pf6N2E2_53 are separated by 224 nucleotides Pf6N2E2_53 and Pf6N2E2_6096 are separated by 146 nucleotides Pf6N2E2_6096 and Pf6N2E2_6097 are separated by 82 nucleotides Pf6N2E2_6097 and Pf6N2E2_6098 are separated by 56 nucleotides Pf6N2E2_6098 and Pf6N2E2_6099 are separated by 78 nucleotides Pf6N2E2_6099 and Pf6N2E2_54 are separated by 184 nucleotides
Pf6N2E2_52 - Excinuclease ABC subunit B, at 90,316 to 92,331
_52
Pf6N2E2_53 - Glutamyl-tRNA synthetase (EC 6.1.1.17) @ Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24), at 92,556 to 94,037
_53
Pf6N2E2_6096 - tRNA-Ala-GGC, at 94,184 to 94,256
_6096
Pf6N2E2_6097 - tRNA-Glu-TTC, at 94,339 to 94,411
_6097
Pf6N2E2_6098 - tRNA-Ala-GGC, at 94,468 to 94,540
_6098
Pf6N2E2_6099 - tRNA-Glu-TTC, at 94,619 to 94,691
_6099
Pf6N2E2_54 - Transcriptional regulator, TetR family, at 94,876 to 95,415
_54
Position (kb) 92
93
94
95 Strain fitness (log2 ratio) -1
0
1 at 91.733 kb on - strand, within Pf6N2E2_52 at 92.177 kb on - strand at 92.243 kb on - strand at 92.536 kb on + strand at 92.536 kb on + strand at 94.263 kb on + strand at 94.263 kb on + strand at 94.263 kb on + strand at 94.324 kb on + strand at 94.546 kb on + strand at 94.546 kb on + strand at 94.663 kb on + strand, within Pf6N2E2_6099 at 94.718 kb on + strand at 94.718 kb on + strand at 94.718 kb on + strand at 95.008 kb on + strand, within Pf6N2E2_54
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 91,733 - Pf6N2E2_52 0.70 +0.9 92,177 - +0.7 92,243 - -0.2 92,536 + +0.1 92,536 + -0.9 94,263 + +1.3 94,263 + +1.1 94,263 + +1.0 94,324 + -1.2 94,546 + +0.1 94,546 + -0.0 94,663 + Pf6N2E2_6099 0.60 +0.0 94,718 + -0.2 94,718 + -0.3 94,718 + -0.8 95,008 + Pf6N2E2_54 0.24 +0.5
Or see this region's nucleotide sequence