Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4917 and Pf6N2E2_4918 overlap by 2 nucleotides Pf6N2E2_4918 and Pf6N2E2_4919 overlap by 4 nucleotides Pf6N2E2_4919 and Pf6N2E2_4920 are separated by 46 nucleotides Pf6N2E2_4920 and Pf6N2E2_4921 are separated by 16 nucleotides
Pf6N2E2_4917 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,672,271 to 5,672,762
_4917
Pf6N2E2_4918 - Ribonucleotide reductase transcriptional regulator NrdR, at 5,672,761 to 5,673,327
_4918
Pf6N2E2_4919 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 5,673,324 to 5,674,457
_4919
Pf6N2E2_4920 - Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9), at 5,674,504 to 5,675,169
_4920
Pf6N2E2_4921 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25), at 5,675,186 to 5,676,277
_4921
Position (kb)
5673
5674
5675 Strain fitness (log2 ratio)
-2
-1
0
1 at 5672.478 kb on + strand, within Pf6N2E2_4917 at 5672.478 kb on + strand, within Pf6N2E2_4917 at 5672.478 kb on + strand, within Pf6N2E2_4917 at 5672.479 kb on - strand, within Pf6N2E2_4917 at 5672.479 kb on - strand, within Pf6N2E2_4917 at 5672.596 kb on - strand, within Pf6N2E2_4917 at 5672.596 kb on - strand, within Pf6N2E2_4917 at 5672.775 kb on - strand at 5672.786 kb on + strand at 5672.786 kb on + strand at 5672.786 kb on + strand at 5672.787 kb on - strand at 5672.787 kb on - strand at 5672.787 kb on - strand at 5672.787 kb on - strand at 5672.788 kb on + strand at 5672.793 kb on + strand at 5672.794 kb on - strand at 5672.800 kb on + strand at 5672.800 kb on + strand at 5672.800 kb on + strand at 5672.801 kb on - strand at 5672.801 kb on - strand at 5672.801 kb on - strand at 5673.232 kb on + strand, within Pf6N2E2_4918 at 5673.232 kb on + strand, within Pf6N2E2_4918 at 5673.253 kb on + strand, within Pf6N2E2_4918 at 5673.363 kb on + strand at 5673.363 kb on + strand at 5673.363 kb on + strand at 5673.363 kb on + strand at 5673.363 kb on + strand at 5674.453 kb on + strand at 5674.453 kb on + strand at 5674.453 kb on + strand at 5674.453 kb on + strand at 5674.454 kb on - strand at 5674.454 kb on - strand at 5674.454 kb on - strand at 5674.454 kb on - strand at 5674.455 kb on + strand at 5674.455 kb on + strand at 5675.155 kb on + strand at 5675.197 kb on + strand at 5675.197 kb on + strand at 5675.197 kb on + strand at 5675.198 kb on - strand at 5675.198 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 5,672,478 + Pf6N2E2_4917 0.42 -0.6 5,672,478 + Pf6N2E2_4917 0.42 -0.0 5,672,478 + Pf6N2E2_4917 0.42 +1.2 5,672,479 - Pf6N2E2_4917 0.42 -1.3 5,672,479 - Pf6N2E2_4917 0.42 -0.1 5,672,596 - Pf6N2E2_4917 0.66 +0.5 5,672,596 - Pf6N2E2_4917 0.66 -0.3 5,672,775 - -0.0 5,672,786 + -1.1 5,672,786 + +1.7 5,672,786 + +0.7 5,672,787 - +0.2 5,672,787 - -0.3 5,672,787 - -0.6 5,672,787 - -0.0 5,672,788 + -0.6 5,672,793 + -0.1 5,672,794 - -0.1 5,672,800 + -2.0 5,672,800 + -0.0 5,672,800 + -2.0 5,672,801 - -0.4 5,672,801 - -0.4 5,672,801 - -0.3 5,673,232 + Pf6N2E2_4918 0.83 -2.2 5,673,232 + Pf6N2E2_4918 0.83 +0.3 5,673,253 + Pf6N2E2_4918 0.87 +0.3 5,673,363 + +0.3 5,673,363 + -0.7 5,673,363 + +0.2 5,673,363 + -0.3 5,673,363 + +0.5 5,674,453 + +0.3 5,674,453 + -0.3 5,674,453 + -0.2 5,674,453 + +0.6 5,674,454 - -0.5 5,674,454 - +0.7 5,674,454 - +1.5 5,674,454 - -0.3 5,674,455 + -0.8 5,674,455 + +0.4 5,675,155 + +0.8 5,675,197 + -0.7 5,675,197 + -1.0 5,675,197 + +0.8 5,675,198 - +1.1 5,675,198 - +0.0
Or see this region's nucleotide sequence