Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3829 and Pf6N2E2_3830 are separated by 39 nucleotides Pf6N2E2_3830 and Pf6N2E2_3831 are separated by 1 nucleotides Pf6N2E2_3831 and Pf6N2E2_3832 are separated by 182 nucleotides Pf6N2E2_3832 and Pf6N2E2_3833 are separated by 177 nucleotides Pf6N2E2_3833 and Pf6N2E2_3834 overlap by 45 nucleotides
Pf6N2E2_3829 - Protein export cytoplasm chaperone protein (SecB, maintains protein to be exported in unfolded state), at 4,463,887 to 4,464,369
_3829
Pf6N2E2_3830 - Glutaredoxin 3 (Grx3), at 4,464,409 to 4,464,663
_3830
Pf6N2E2_3831 - FIG136845: Rhodanese-related sulfurtransferase, at 4,464,665 to 4,465,078
_3831
Pf6N2E2_3832 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1), at 4,465,261 to 4,466,760
_3832
Pf6N2E2_3833 - hypothetical protein, at 4,466,938 to 4,467,123
_3833
Pf6N2E2_3834 - Lipoprotein NlpD, at 4,467,079 to 4,468,371
_3834
Position (kb)
4465
4466
4467 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4464.299 kb on + strand, within Pf6N2E2_3829 at 4464.299 kb on + strand, within Pf6N2E2_3829 at 4464.299 kb on + strand, within Pf6N2E2_3829 at 4464.299 kb on + strand, within Pf6N2E2_3829 at 4464.299 kb on + strand, within Pf6N2E2_3829 at 4464.300 kb on - strand, within Pf6N2E2_3829 at 4464.300 kb on - strand, within Pf6N2E2_3829 at 4464.300 kb on - strand, within Pf6N2E2_3829 at 4464.300 kb on - strand, within Pf6N2E2_3829 at 4464.300 kb on - strand, within Pf6N2E2_3829 at 4464.383 kb on + strand at 4464.384 kb on - strand at 4464.384 kb on - strand at 4464.384 kb on - strand at 4464.384 kb on - strand at 4464.384 kb on - strand at 4464.435 kb on + strand, within Pf6N2E2_3830 at 4464.605 kb on + strand, within Pf6N2E2_3830 at 4464.605 kb on + strand, within Pf6N2E2_3830 at 4464.605 kb on + strand, within Pf6N2E2_3830 at 4464.605 kb on + strand, within Pf6N2E2_3830 at 4464.606 kb on - strand, within Pf6N2E2_3830 at 4464.606 kb on - strand, within Pf6N2E2_3830 at 4464.606 kb on - strand, within Pf6N2E2_3830 at 4464.623 kb on + strand, within Pf6N2E2_3830 at 4464.632 kb on + strand, within Pf6N2E2_3830 at 4464.704 kb on - strand at 4464.704 kb on - strand at 4465.042 kb on - strand at 4465.100 kb on - strand at 4465.115 kb on - strand at 4465.115 kb on - strand at 4465.116 kb on + strand at 4465.116 kb on + strand at 4465.116 kb on + strand at 4465.116 kb on + strand at 4465.116 kb on + strand at 4465.116 kb on + strand at 4465.117 kb on - strand at 4465.117 kb on - strand at 4465.117 kb on - strand at 4465.117 kb on - strand at 4465.117 kb on - strand at 4465.117 kb on - strand at 4465.170 kb on + strand at 4465.170 kb on + strand at 4465.176 kb on + strand at 4465.177 kb on - strand at 4465.236 kb on + strand at 4466.769 kb on + strand at 4466.770 kb on - strand at 4466.770 kb on - strand at 4466.770 kb on - strand at 4466.770 kb on - strand at 4466.880 kb on + strand at 4467.042 kb on + strand, within Pf6N2E2_3833 at 4467.042 kb on + strand, within Pf6N2E2_3833 at 4467.043 kb on - strand, within Pf6N2E2_3833 at 4467.043 kb on - strand, within Pf6N2E2_3833 at 4467.045 kb on + strand, within Pf6N2E2_3833 at 4467.045 kb on + strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.078 kb on + strand, within Pf6N2E2_3833 at 4467.079 kb on - strand at 4467.079 kb on - strand at 4467.440 kb on - strand, within Pf6N2E2_3834 at 4467.440 kb on - strand, within Pf6N2E2_3834 at 4467.496 kb on + strand, within Pf6N2E2_3834 at 4467.497 kb on - strand, within Pf6N2E2_3834 at 4467.497 kb on - strand, within Pf6N2E2_3834 at 4467.499 kb on + strand, within Pf6N2E2_3834 at 4467.499 kb on + strand, within Pf6N2E2_3834 at 4467.500 kb on - strand, within Pf6N2E2_3834 at 4467.500 kb on - strand, within Pf6N2E2_3834
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 4,464,299 + Pf6N2E2_3829 0.85 +0.8 4,464,299 + Pf6N2E2_3829 0.85 -1.8 4,464,299 + Pf6N2E2_3829 0.85 +0.3 4,464,299 + Pf6N2E2_3829 0.85 +0.8 4,464,299 + Pf6N2E2_3829 0.85 -0.8 4,464,300 - Pf6N2E2_3829 0.86 +0.7 4,464,300 - Pf6N2E2_3829 0.86 -0.3 4,464,300 - Pf6N2E2_3829 0.86 +0.2 4,464,300 - Pf6N2E2_3829 0.86 -0.2 4,464,300 - Pf6N2E2_3829 0.86 -0.1 4,464,383 + +1.9 4,464,384 - +1.2 4,464,384 - -0.0 4,464,384 - +0.1 4,464,384 - +0.1 4,464,384 - -0.2 4,464,435 + Pf6N2E2_3830 0.10 -0.5 4,464,605 + Pf6N2E2_3830 0.77 -0.3 4,464,605 + Pf6N2E2_3830 0.77 +0.2 4,464,605 + Pf6N2E2_3830 0.77 -0.1 4,464,605 + Pf6N2E2_3830 0.77 +0.0 4,464,606 - Pf6N2E2_3830 0.77 -0.4 4,464,606 - Pf6N2E2_3830 0.77 +1.2 4,464,606 - Pf6N2E2_3830 0.77 -0.7 4,464,623 + Pf6N2E2_3830 0.84 +0.3 4,464,632 + Pf6N2E2_3830 0.87 +0.2 4,464,704 - -1.4 4,464,704 - -0.9 4,465,042 - -1.2 4,465,100 - +0.4 4,465,115 - +0.2 4,465,115 - +0.4 4,465,116 + -0.5 4,465,116 + +0.9 4,465,116 + -0.4 4,465,116 + +0.6 4,465,116 + +0.5 4,465,116 + -0.0 4,465,117 - -1.4 4,465,117 - +0.0 4,465,117 - -0.5 4,465,117 - -0.2 4,465,117 - -0.3 4,465,117 - -1.0 4,465,170 + +0.1 4,465,170 + +0.6 4,465,176 + -0.0 4,465,177 - -0.6 4,465,236 + -2.2 4,466,769 + -2.6 4,466,770 - +0.2 4,466,770 - +0.0 4,466,770 - +0.6 4,466,770 - +0.0 4,466,880 + -0.1 4,467,042 + Pf6N2E2_3833 0.56 +0.1 4,467,042 + Pf6N2E2_3833 0.56 -2.2 4,467,043 - Pf6N2E2_3833 0.56 -0.5 4,467,043 - Pf6N2E2_3833 0.56 +1.2 4,467,045 + Pf6N2E2_3833 0.58 +0.8 4,467,045 + Pf6N2E2_3833 0.58 -0.8 4,467,046 - Pf6N2E2_3833 0.58 -1.3 4,467,046 - Pf6N2E2_3833 0.58 -2.2 4,467,046 - Pf6N2E2_3833 0.58 -0.7 4,467,046 - Pf6N2E2_3833 0.58 -2.2 4,467,046 - Pf6N2E2_3833 0.58 -0.8 4,467,078 + Pf6N2E2_3833 0.75 -1.8 4,467,079 - -1.1 4,467,079 - -1.9 4,467,440 - Pf6N2E2_3834 0.28 -0.2 4,467,440 - Pf6N2E2_3834 0.28 -0.6 4,467,496 + Pf6N2E2_3834 0.32 +0.2 4,467,497 - Pf6N2E2_3834 0.32 -1.2 4,467,497 - Pf6N2E2_3834 0.32 -0.4 4,467,499 + Pf6N2E2_3834 0.32 -1.2 4,467,499 + Pf6N2E2_3834 0.32 -0.0 4,467,500 - Pf6N2E2_3834 0.33 -0.8 4,467,500 - Pf6N2E2_3834 0.33 -2.3
Or see this region's nucleotide sequence