Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3780

Experiment: RCH2_defined

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3778 and Pf6N2E2_3779 are separated by 155 nucleotidesPf6N2E2_3779 and Pf6N2E2_3780 overlap by 1 nucleotidesPf6N2E2_3780 and Pf6N2E2_3781 are separated by 3 nucleotides Pf6N2E2_3778 - FIG00954300: hypothetical protein, at 4,401,979 to 4,402,254 _3778 Pf6N2E2_3779 - Ubiquinone/menaquinone biosynthesis methyltransferase UbiE (EC 2.1.1.-), at 4,402,410 to 4,403,180 _3779 Pf6N2E2_3780 - Protein YigP (COG3165) clustered with ubiquinone biosynthetic genes, at 4,403,180 to 4,403,797 _3780 Pf6N2E2_3781 - Ubiquinone biosynthesis monooxygenase UbiB, at 4,403,801 to 4,405,405 _3781 Position (kb) 4403 4404Strain fitness (log2 ratio) -1 0 1 2at 4402.243 kb on + strandat 4402.245 kb on + strandat 4402.245 kb on + strandat 4402.245 kb on + strandat 4402.246 kb on - strandat 4402.246 kb on - strandat 4402.246 kb on - strandat 4402.336 kb on + strandat 4402.336 kb on + strandat 4402.336 kb on + strandat 4402.336 kb on + strandat 4402.336 kb on + strandat 4402.336 kb on + strandat 4402.337 kb on - strandat 4402.337 kb on - strandat 4402.337 kb on - strandat 4402.337 kb on - strandat 4402.375 kb on + strandat 4403.654 kb on + strand, within Pf6N2E2_3780at 4403.654 kb on + strand, within Pf6N2E2_3780

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined
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4,402,243 + -1.7
4,402,245 + -1.1
4,402,245 + -0.4
4,402,245 + +1.8
4,402,246 - -0.2
4,402,246 - -0.3
4,402,246 - +0.6
4,402,336 + -1.7
4,402,336 + -0.3
4,402,336 + -1.0
4,402,336 + +1.8
4,402,336 + -1.3
4,402,336 + +0.2
4,402,337 - +0.3
4,402,337 - -1.3
4,402,337 - -0.7
4,402,337 - +0.2
4,402,375 + -0.1
4,403,654 + Pf6N2E2_3780 0.77 -0.4
4,403,654 + Pf6N2E2_3780 0.77 +1.2

Or see this region's nucleotide sequence