Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3777 and Pf6N2E2_3778 are separated by 262 nucleotides Pf6N2E2_3778 and Pf6N2E2_3779 are separated by 155 nucleotides Pf6N2E2_3779 and Pf6N2E2_3780 overlap by 1 nucleotides Pf6N2E2_3780 and Pf6N2E2_3781 are separated by 3 nucleotides
Pf6N2E2_3777 - polyhydroxyalkanoate granule-associated protein PhaI, at 4,401,294 to 4,401,716
_3777
Pf6N2E2_3778 - FIG00954300: hypothetical protein, at 4,401,979 to 4,402,254
_3778
Pf6N2E2_3779 - Ubiquinone/menaquinone biosynthesis methyltransferase UbiE (EC 2.1.1.-), at 4,402,410 to 4,403,180
_3779
Pf6N2E2_3780 - Protein YigP (COG3165) clustered with ubiquinone biosynthetic genes, at 4,403,180 to 4,403,797
_3780
Pf6N2E2_3781 - Ubiquinone biosynthesis monooxygenase UbiB, at 4,403,801 to 4,405,405
_3781
Position (kb)
4402
4403
4404 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4401.445 kb on - strand, within Pf6N2E2_3777 at 4401.445 kb on - strand, within Pf6N2E2_3777 at 4401.568 kb on - strand, within Pf6N2E2_3777 at 4401.568 kb on - strand, within Pf6N2E2_3777 at 4401.650 kb on - strand, within Pf6N2E2_3777 at 4401.650 kb on - strand, within Pf6N2E2_3777 at 4401.650 kb on - strand, within Pf6N2E2_3777 at 4401.780 kb on + strand at 4401.780 kb on + strand at 4401.781 kb on - strand at 4401.789 kb on + strand at 4401.789 kb on + strand at 4401.789 kb on + strand at 4401.790 kb on - strand at 4401.790 kb on - strand at 4401.790 kb on - strand at 4401.790 kb on - strand at 4401.790 kb on - strand at 4401.790 kb on - strand at 4401.794 kb on - strand at 4401.807 kb on + strand at 4401.807 kb on + strand at 4401.808 kb on - strand at 4401.901 kb on - strand at 4401.908 kb on - strand at 4401.960 kb on - strand at 4401.960 kb on - strand at 4401.960 kb on - strand at 4401.960 kb on - strand at 4401.960 kb on - strand at 4401.960 kb on - strand at 4401.960 kb on - strand at 4402.160 kb on + strand, within Pf6N2E2_3778 at 4402.160 kb on + strand, within Pf6N2E2_3778 at 4402.161 kb on - strand, within Pf6N2E2_3778 at 4402.165 kb on - strand, within Pf6N2E2_3778 at 4402.243 kb on + strand at 4402.245 kb on + strand at 4402.245 kb on + strand at 4402.245 kb on + strand at 4402.246 kb on - strand at 4402.246 kb on - strand at 4402.246 kb on - strand at 4402.336 kb on + strand at 4402.336 kb on + strand at 4402.336 kb on + strand at 4402.336 kb on + strand at 4402.336 kb on + strand at 4402.336 kb on + strand at 4402.337 kb on - strand at 4402.337 kb on - strand at 4402.337 kb on - strand at 4402.337 kb on - strand at 4402.375 kb on + strand at 4403.654 kb on + strand, within Pf6N2E2_3780 at 4403.654 kb on + strand, within Pf6N2E2_3780
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 4,401,445 - Pf6N2E2_3777 0.36 +2.0 4,401,445 - Pf6N2E2_3777 0.36 +0.4 4,401,568 - Pf6N2E2_3777 0.65 +0.2 4,401,568 - Pf6N2E2_3777 0.65 -0.1 4,401,650 - Pf6N2E2_3777 0.84 +0.1 4,401,650 - Pf6N2E2_3777 0.84 +0.1 4,401,650 - Pf6N2E2_3777 0.84 -0.5 4,401,780 + +1.2 4,401,780 + -0.1 4,401,781 - +0.1 4,401,789 + -1.3 4,401,789 + +0.1 4,401,789 + -0.9 4,401,790 - +0.2 4,401,790 - -0.3 4,401,790 - +1.0 4,401,790 - +2.1 4,401,790 - -0.1 4,401,790 - -1.8 4,401,794 - +1.5 4,401,807 + +0.6 4,401,807 + -0.3 4,401,808 - -0.4 4,401,901 - +0.1 4,401,908 - +0.4 4,401,960 - -0.4 4,401,960 - -0.3 4,401,960 - +0.3 4,401,960 - -0.2 4,401,960 - +0.0 4,401,960 - +0.5 4,401,960 - -0.0 4,402,160 + Pf6N2E2_3778 0.66 -0.1 4,402,160 + Pf6N2E2_3778 0.66 +0.4 4,402,161 - Pf6N2E2_3778 0.66 +0.5 4,402,165 - Pf6N2E2_3778 0.67 +0.2 4,402,243 + -1.7 4,402,245 + -1.1 4,402,245 + -0.4 4,402,245 + +1.8 4,402,246 - -0.2 4,402,246 - -0.3 4,402,246 - +0.6 4,402,336 + -1.7 4,402,336 + -0.3 4,402,336 + -1.0 4,402,336 + +1.8 4,402,336 + -1.3 4,402,336 + +0.2 4,402,337 - +0.3 4,402,337 - -1.3 4,402,337 - -0.7 4,402,337 - +0.2 4,402,375 + -0.1 4,403,654 + Pf6N2E2_3780 0.77 -0.4 4,403,654 + Pf6N2E2_3780 0.77 +1.2
Or see this region's nucleotide sequence