Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3252 and Pf6N2E2_3253 are separated by 123 nucleotides Pf6N2E2_3253 and Pf6N2E2_3254 are separated by 127 nucleotides Pf6N2E2_3254 and Pf6N2E2_3255 are separated by 22 nucleotides Pf6N2E2_3255 and Pf6N2E2_3256 are separated by 223 nucleotides Pf6N2E2_3256 and Pf6N2E2_3257 overlap by 4 nucleotides
Pf6N2E2_3252 - Histidinol dehydrogenase (EC 1.1.1.23), at 3,762,472 to 3,763,809
_3252
Pf6N2E2_3253 - ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) , at 3,763,933 to 3,764,568
_3253
Pf6N2E2_3254 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7), at 3,764,696 to 3,765,961
_3254
Pf6N2E2_3255 - YrbA protein, at 3,765,984 to 3,766,223
_3255
Pf6N2E2_3256 - Uncharacterized protein YrbB, at 3,766,447 to 3,766,764
_3256
Pf6N2E2_3257 - Uncharacterized ABC transporter, auxiliary component YrbC, at 3,766,761 to 3,767,414
_3257
Position (kb)
3764
3765
3766 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3763.852 kb on + strand at 3763.853 kb on - strand at 3763.853 kb on - strand at 3763.889 kb on + strand at 3764.159 kb on + strand, within Pf6N2E2_3253 at 3764.159 kb on + strand, within Pf6N2E2_3253 at 3764.159 kb on + strand, within Pf6N2E2_3253 at 3764.160 kb on - strand, within Pf6N2E2_3253 at 3764.160 kb on - strand, within Pf6N2E2_3253 at 3764.160 kb on - strand, within Pf6N2E2_3253 at 3764.199 kb on - strand, within Pf6N2E2_3253 at 3764.201 kb on + strand, within Pf6N2E2_3253 at 3764.202 kb on - strand, within Pf6N2E2_3253 at 3764.202 kb on - strand, within Pf6N2E2_3253 at 3764.396 kb on + strand, within Pf6N2E2_3253 at 3764.396 kb on + strand, within Pf6N2E2_3253 at 3764.396 kb on + strand, within Pf6N2E2_3253 at 3764.396 kb on + strand, within Pf6N2E2_3253 at 3764.585 kb on + strand at 3764.593 kb on + strand at 3764.594 kb on - strand at 3764.594 kb on - strand at 3764.594 kb on - strand at 3764.594 kb on - strand at 3764.697 kb on + strand at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.076 kb on - strand, within Pf6N2E2_3255 at 3766.195 kb on - strand, within Pf6N2E2_3255 at 3766.195 kb on - strand, within Pf6N2E2_3255 at 3766.195 kb on - strand, within Pf6N2E2_3255 at 3766.213 kb on - strand at 3766.317 kb on + strand at 3766.489 kb on + strand, within Pf6N2E2_3256
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 3,763,852 + -3.1 3,763,853 - +0.1 3,763,853 - +0.8 3,763,889 + -4.0 3,764,159 + Pf6N2E2_3253 0.36 -4.2 3,764,159 + Pf6N2E2_3253 0.36 -2.0 3,764,159 + Pf6N2E2_3253 0.36 -3.5 3,764,160 - Pf6N2E2_3253 0.36 -2.7 3,764,160 - Pf6N2E2_3253 0.36 -4.4 3,764,160 - Pf6N2E2_3253 0.36 -4.0 3,764,199 - Pf6N2E2_3253 0.42 -1.7 3,764,201 + Pf6N2E2_3253 0.42 -2.0 3,764,202 - Pf6N2E2_3253 0.42 -4.4 3,764,202 - Pf6N2E2_3253 0.42 -2.4 3,764,396 + Pf6N2E2_3253 0.73 -3.6 3,764,396 + Pf6N2E2_3253 0.73 -3.8 3,764,396 + Pf6N2E2_3253 0.73 -4.0 3,764,396 + Pf6N2E2_3253 0.73 -1.2 3,764,585 + -2.0 3,764,593 + -2.9 3,764,594 - -0.1 3,764,594 - +0.2 3,764,594 - +0.9 3,764,594 - +0.2 3,764,697 + +0.1 3,766,076 - Pf6N2E2_3255 0.38 +0.1 3,766,076 - Pf6N2E2_3255 0.38 +0.5 3,766,076 - Pf6N2E2_3255 0.38 -2.0 3,766,076 - Pf6N2E2_3255 0.38 -0.7 3,766,076 - Pf6N2E2_3255 0.38 +0.5 3,766,076 - Pf6N2E2_3255 0.38 -0.5 3,766,195 - Pf6N2E2_3255 0.88 +0.8 3,766,195 - Pf6N2E2_3255 0.88 -1.2 3,766,195 - Pf6N2E2_3255 0.88 -0.9 3,766,213 - -2.1 3,766,317 + +0.0 3,766,489 + Pf6N2E2_3256 0.13 -2.6
Or see this region's nucleotide sequence