Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3254

Experiment: RCH2_defined

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3252 and Pf6N2E2_3253 are separated by 123 nucleotidesPf6N2E2_3253 and Pf6N2E2_3254 are separated by 127 nucleotidesPf6N2E2_3254 and Pf6N2E2_3255 are separated by 22 nucleotidesPf6N2E2_3255 and Pf6N2E2_3256 are separated by 223 nucleotidesPf6N2E2_3256 and Pf6N2E2_3257 overlap by 4 nucleotides Pf6N2E2_3252 - Histidinol dehydrogenase (EC 1.1.1.23), at 3,762,472 to 3,763,809 _3252 Pf6N2E2_3253 - ATP phosphoribosyltransferase (EC 2.4.2.17) (from data), at 3,763,933 to 3,764,568 _3253 Pf6N2E2_3254 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7), at 3,764,696 to 3,765,961 _3254 Pf6N2E2_3255 - YrbA protein, at 3,765,984 to 3,766,223 _3255 Pf6N2E2_3256 - Uncharacterized protein YrbB, at 3,766,447 to 3,766,764 _3256 Pf6N2E2_3257 - Uncharacterized ABC transporter, auxiliary component YrbC, at 3,766,761 to 3,767,414 _3257 Position (kb) 3764 3765 3766Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3763.852 kb on + strandat 3763.853 kb on - strandat 3763.853 kb on - strandat 3763.889 kb on + strandat 3764.159 kb on + strand, within Pf6N2E2_3253at 3764.159 kb on + strand, within Pf6N2E2_3253at 3764.159 kb on + strand, within Pf6N2E2_3253at 3764.160 kb on - strand, within Pf6N2E2_3253at 3764.160 kb on - strand, within Pf6N2E2_3253at 3764.160 kb on - strand, within Pf6N2E2_3253at 3764.199 kb on - strand, within Pf6N2E2_3253at 3764.201 kb on + strand, within Pf6N2E2_3253at 3764.202 kb on - strand, within Pf6N2E2_3253at 3764.202 kb on - strand, within Pf6N2E2_3253at 3764.396 kb on + strand, within Pf6N2E2_3253at 3764.396 kb on + strand, within Pf6N2E2_3253at 3764.396 kb on + strand, within Pf6N2E2_3253at 3764.396 kb on + strand, within Pf6N2E2_3253at 3764.585 kb on + strandat 3764.593 kb on + strandat 3764.594 kb on - strandat 3764.594 kb on - strandat 3764.594 kb on - strandat 3764.594 kb on - strandat 3764.697 kb on + strandat 3766.076 kb on - strand, within Pf6N2E2_3255at 3766.076 kb on - strand, within Pf6N2E2_3255at 3766.076 kb on - strand, within Pf6N2E2_3255at 3766.076 kb on - strand, within Pf6N2E2_3255at 3766.076 kb on - strand, within Pf6N2E2_3255at 3766.076 kb on - strand, within Pf6N2E2_3255at 3766.195 kb on - strand, within Pf6N2E2_3255at 3766.195 kb on - strand, within Pf6N2E2_3255at 3766.195 kb on - strand, within Pf6N2E2_3255at 3766.213 kb on - strandat 3766.317 kb on + strandat 3766.489 kb on + strand, within Pf6N2E2_3256

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined
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3,763,852 + -3.1
3,763,853 - +0.1
3,763,853 - +0.8
3,763,889 + -4.0
3,764,159 + Pf6N2E2_3253 0.36 -4.2
3,764,159 + Pf6N2E2_3253 0.36 -2.0
3,764,159 + Pf6N2E2_3253 0.36 -3.5
3,764,160 - Pf6N2E2_3253 0.36 -2.7
3,764,160 - Pf6N2E2_3253 0.36 -4.4
3,764,160 - Pf6N2E2_3253 0.36 -4.0
3,764,199 - Pf6N2E2_3253 0.42 -1.7
3,764,201 + Pf6N2E2_3253 0.42 -2.0
3,764,202 - Pf6N2E2_3253 0.42 -4.4
3,764,202 - Pf6N2E2_3253 0.42 -2.4
3,764,396 + Pf6N2E2_3253 0.73 -3.6
3,764,396 + Pf6N2E2_3253 0.73 -3.8
3,764,396 + Pf6N2E2_3253 0.73 -4.0
3,764,396 + Pf6N2E2_3253 0.73 -1.2
3,764,585 + -2.0
3,764,593 + -2.9
3,764,594 - -0.1
3,764,594 - +0.2
3,764,594 - +0.9
3,764,594 - +0.2
3,764,697 + +0.1
3,766,076 - Pf6N2E2_3255 0.38 +0.1
3,766,076 - Pf6N2E2_3255 0.38 +0.5
3,766,076 - Pf6N2E2_3255 0.38 -2.0
3,766,076 - Pf6N2E2_3255 0.38 -0.7
3,766,076 - Pf6N2E2_3255 0.38 +0.5
3,766,076 - Pf6N2E2_3255 0.38 -0.5
3,766,195 - Pf6N2E2_3255 0.88 +0.8
3,766,195 - Pf6N2E2_3255 0.88 -1.2
3,766,195 - Pf6N2E2_3255 0.88 -0.9
3,766,213 - -2.1
3,766,317 + +0.0
3,766,489 + Pf6N2E2_3256 0.13 -2.6

Or see this region's nucleotide sequence