Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3191 and Pf6N2E2_3192 are separated by 1 nucleotides Pf6N2E2_3192 and Pf6N2E2_3193 are separated by 144 nucleotides Pf6N2E2_3193 and Pf6N2E2_3194 are separated by 310 nucleotides Pf6N2E2_3194 and Pf6N2E2_3195 are separated by 117 nucleotides
Pf6N2E2_3191 - Serine acetyltransferase (EC 2.3.1.30), at 3,684,848 to 3,685,624
_3191
Pf6N2E2_3192 - tRNA:Cm32/Um32 methyltransferase, at 3,685,626 to 3,686,396
_3192
Pf6N2E2_3193 - Inositol-1-monophosphatase (EC 3.1.3.25), at 3,686,541 to 3,687,359
_3193
Pf6N2E2_3194 - probable exported protein YPO1624, at 3,687,670 to 3,688,218
_3194
Pf6N2E2_3195 - Protein-export membrane protein SecF (TC 3.A.5.1.1), at 3,688,336 to 3,689,250
_3195
Position (kb)
3686
3687
3688 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3685.641 kb on - strand at 3685.693 kb on - strand at 3685.693 kb on - strand at 3685.694 kb on + strand at 3685.694 kb on + strand at 3685.694 kb on + strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.730 kb on - strand, within Pf6N2E2_3192 at 3685.816 kb on + strand, within Pf6N2E2_3192 at 3685.817 kb on - strand, within Pf6N2E2_3192 at 3685.817 kb on - strand, within Pf6N2E2_3192 at 3685.817 kb on - strand, within Pf6N2E2_3192 at 3685.933 kb on + strand, within Pf6N2E2_3192 at 3685.934 kb on - strand, within Pf6N2E2_3192 at 3685.934 kb on - strand, within Pf6N2E2_3192 at 3685.934 kb on - strand, within Pf6N2E2_3192 at 3686.141 kb on + strand, within Pf6N2E2_3192 at 3686.364 kb on - strand at 3686.418 kb on + strand at 3686.446 kb on + strand at 3686.446 kb on + strand at 3686.457 kb on + strand at 3687.358 kb on - strand at 3687.435 kb on + strand at 3687.616 kb on - strand at 3687.783 kb on - strand, within Pf6N2E2_3194 at 3687.783 kb on - strand, within Pf6N2E2_3194 at 3687.795 kb on - strand, within Pf6N2E2_3194 at 3687.799 kb on + strand, within Pf6N2E2_3194 at 3687.799 kb on + strand, within Pf6N2E2_3194 at 3687.800 kb on - strand, within Pf6N2E2_3194 at 3687.800 kb on - strand, within Pf6N2E2_3194 at 3687.800 kb on - strand, within Pf6N2E2_3194 at 3687.800 kb on - strand, within Pf6N2E2_3194 at 3687.854 kb on + strand, within Pf6N2E2_3194 at 3687.854 kb on + strand, within Pf6N2E2_3194 at 3687.854 kb on + strand, within Pf6N2E2_3194 at 3687.855 kb on - strand, within Pf6N2E2_3194 at 3687.855 kb on - strand, within Pf6N2E2_3194 at 3687.855 kb on - strand, within Pf6N2E2_3194 at 3687.855 kb on - strand, within Pf6N2E2_3194 at 3687.855 kb on - strand, within Pf6N2E2_3194 at 3687.855 kb on - strand, within Pf6N2E2_3194 at 3687.899 kb on + strand, within Pf6N2E2_3194 at 3688.108 kb on + strand, within Pf6N2E2_3194 at 3688.108 kb on + strand, within Pf6N2E2_3194 at 3688.109 kb on - strand, within Pf6N2E2_3194 at 3688.109 kb on - strand, within Pf6N2E2_3194 at 3688.109 kb on - strand, within Pf6N2E2_3194 at 3688.109 kb on - strand, within Pf6N2E2_3194 at 3688.135 kb on + strand, within Pf6N2E2_3194 at 3688.136 kb on - strand, within Pf6N2E2_3194 at 3688.145 kb on + strand, within Pf6N2E2_3194 at 3688.145 kb on + strand, within Pf6N2E2_3194 at 3688.145 kb on + strand, within Pf6N2E2_3194 at 3688.145 kb on + strand, within Pf6N2E2_3194 at 3688.146 kb on - strand, within Pf6N2E2_3194 at 3688.171 kb on + strand at 3688.171 kb on + strand at 3688.171 kb on + strand at 3688.247 kb on + strand at 3688.247 kb on + strand at 3688.248 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 3,685,641 - +0.5 3,685,693 - +0.5 3,685,693 - +0.8 3,685,694 + +1.1 3,685,694 + -1.5 3,685,694 + +0.2 3,685,695 - +0.1 3,685,695 - -0.9 3,685,695 - -1.2 3,685,695 - +1.3 3,685,695 - +0.5 3,685,695 - +0.2 3,685,695 - -0.2 3,685,695 - +0.9 3,685,695 - +0.7 3,685,695 - -0.1 3,685,730 - Pf6N2E2_3192 0.13 +0.9 3,685,816 + Pf6N2E2_3192 0.25 +1.1 3,685,817 - Pf6N2E2_3192 0.25 -0.9 3,685,817 - Pf6N2E2_3192 0.25 -0.7 3,685,817 - Pf6N2E2_3192 0.25 +0.2 3,685,933 + Pf6N2E2_3192 0.40 -0.6 3,685,934 - Pf6N2E2_3192 0.40 +1.2 3,685,934 - Pf6N2E2_3192 0.40 -0.8 3,685,934 - Pf6N2E2_3192 0.40 -0.8 3,686,141 + Pf6N2E2_3192 0.67 -2.3 3,686,364 - -0.2 3,686,418 + -0.1 3,686,446 + -1.3 3,686,446 + +0.1 3,686,457 + -0.4 3,687,358 - -0.4 3,687,435 + -0.1 3,687,616 - +0.0 3,687,783 - Pf6N2E2_3194 0.21 -1.2 3,687,783 - Pf6N2E2_3194 0.21 +0.3 3,687,795 - Pf6N2E2_3194 0.23 -0.9 3,687,799 + Pf6N2E2_3194 0.23 +0.6 3,687,799 + Pf6N2E2_3194 0.23 -0.3 3,687,800 - Pf6N2E2_3194 0.24 +0.5 3,687,800 - Pf6N2E2_3194 0.24 -0.1 3,687,800 - Pf6N2E2_3194 0.24 -0.1 3,687,800 - Pf6N2E2_3194 0.24 -1.0 3,687,854 + Pf6N2E2_3194 0.34 +0.3 3,687,854 + Pf6N2E2_3194 0.34 -0.0 3,687,854 + Pf6N2E2_3194 0.34 -0.1 3,687,855 - Pf6N2E2_3194 0.34 -0.3 3,687,855 - Pf6N2E2_3194 0.34 +0.7 3,687,855 - Pf6N2E2_3194 0.34 -0.1 3,687,855 - Pf6N2E2_3194 0.34 -0.6 3,687,855 - Pf6N2E2_3194 0.34 +0.7 3,687,855 - Pf6N2E2_3194 0.34 +0.8 3,687,899 + Pf6N2E2_3194 0.42 -0.3 3,688,108 + Pf6N2E2_3194 0.80 -1.0 3,688,108 + Pf6N2E2_3194 0.80 +0.1 3,688,109 - Pf6N2E2_3194 0.80 +0.2 3,688,109 - Pf6N2E2_3194 0.80 -0.0 3,688,109 - Pf6N2E2_3194 0.80 -0.1 3,688,109 - Pf6N2E2_3194 0.80 +0.4 3,688,135 + Pf6N2E2_3194 0.85 -0.8 3,688,136 - Pf6N2E2_3194 0.85 -0.6 3,688,145 + Pf6N2E2_3194 0.87 -0.7 3,688,145 + Pf6N2E2_3194 0.87 -0.6 3,688,145 + Pf6N2E2_3194 0.87 -0.2 3,688,145 + Pf6N2E2_3194 0.87 -0.4 3,688,146 - Pf6N2E2_3194 0.87 -0.7 3,688,171 + +0.7 3,688,171 + -0.5 3,688,171 + +2.2 3,688,247 + -0.0 3,688,247 + +1.0 3,688,248 - +0.2
Or see this region's nucleotide sequence