Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3081 and Pf6N2E2_3082 are separated by 44 nucleotides Pf6N2E2_3082 and Pf6N2E2_3083 overlap by 12 nucleotides Pf6N2E2_3083 and Pf6N2E2_3084 overlap by 1 nucleotides Pf6N2E2_3084 and Pf6N2E2_3085 are separated by 82 nucleotides
Pf6N2E2_3081 - HesA/MoeB/ThiF family protein related to EC-YgdL, at 3,569,148 to 3,569,966
_3081
Pf6N2E2_3082 - hypothetical protein, at 3,570,011 to 3,570,190
_3082
Pf6N2E2_3083 - N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18), at 3,570,179 to 3,571,330
_3083
Pf6N2E2_3084 - Ribosomal RNA large subunit methyltransferase A (EC 2.1.1.51), at 3,571,330 to 3,572,139
_3084
Pf6N2E2_3085 - FIG00954614: hypothetical protein, at 3,572,222 to 3,572,599
_3085
Position (kb)
3570
3571
3572 Strain fitness (log2 ratio)
-1
0
1 at 3569.201 kb on + strand at 3569.380 kb on + strand, within Pf6N2E2_3081 at 3569.380 kb on + strand, within Pf6N2E2_3081 at 3569.381 kb on - strand, within Pf6N2E2_3081 at 3569.381 kb on - strand, within Pf6N2E2_3081 at 3569.433 kb on + strand, within Pf6N2E2_3081 at 3569.433 kb on + strand, within Pf6N2E2_3081 at 3569.433 kb on + strand, within Pf6N2E2_3081 at 3569.434 kb on - strand, within Pf6N2E2_3081 at 3569.434 kb on - strand, within Pf6N2E2_3081 at 3569.626 kb on + strand, within Pf6N2E2_3081 at 3569.626 kb on + strand, within Pf6N2E2_3081 at 3569.626 kb on + strand, within Pf6N2E2_3081 at 3569.627 kb on - strand, within Pf6N2E2_3081 at 3569.627 kb on - strand, within Pf6N2E2_3081 at 3569.918 kb on - strand at 3569.955 kb on - strand at 3569.989 kb on + strand at 3569.989 kb on + strand at 3569.990 kb on - strand at 3569.990 kb on - strand at 3569.990 kb on - strand at 3569.991 kb on + strand at 3569.992 kb on - strand at 3569.992 kb on - strand at 3569.992 kb on - strand at 3569.992 kb on - strand at 3570.100 kb on + strand, within Pf6N2E2_3082 at 3570.101 kb on - strand, within Pf6N2E2_3082 at 3570.102 kb on + strand, within Pf6N2E2_3082 at 3570.102 kb on + strand, within Pf6N2E2_3082 at 3570.103 kb on - strand, within Pf6N2E2_3082 at 3570.107 kb on + strand, within Pf6N2E2_3082 at 3570.107 kb on + strand, within Pf6N2E2_3082 at 3570.107 kb on + strand, within Pf6N2E2_3082 at 3570.107 kb on + strand, within Pf6N2E2_3082 at 3570.141 kb on + strand, within Pf6N2E2_3082 at 3570.161 kb on + strand, within Pf6N2E2_3082 at 3570.161 kb on + strand, within Pf6N2E2_3082 at 3571.525 kb on + strand, within Pf6N2E2_3084 at 3571.609 kb on + strand, within Pf6N2E2_3084 at 3571.609 kb on + strand, within Pf6N2E2_3084 at 3571.609 kb on + strand, within Pf6N2E2_3084 at 3571.610 kb on - strand, within Pf6N2E2_3084 at 3571.610 kb on - strand, within Pf6N2E2_3084 at 3571.612 kb on + strand, within Pf6N2E2_3084 at 3571.612 kb on + strand, within Pf6N2E2_3084 at 3571.613 kb on - strand, within Pf6N2E2_3084 at 3571.613 kb on - strand, within Pf6N2E2_3084 at 3571.613 kb on - strand, within Pf6N2E2_3084 at 3571.909 kb on + strand, within Pf6N2E2_3084 at 3571.909 kb on + strand, within Pf6N2E2_3084 at 3571.909 kb on + strand, within Pf6N2E2_3084 at 3571.910 kb on - strand, within Pf6N2E2_3084 at 3571.910 kb on - strand, within Pf6N2E2_3084 at 3572.117 kb on - strand at 3572.122 kb on + strand at 3572.122 kb on + strand at 3572.123 kb on - strand at 3572.247 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 3,569,201 + +0.2 3,569,380 + Pf6N2E2_3081 0.28 +0.8 3,569,380 + Pf6N2E2_3081 0.28 -0.1 3,569,381 - Pf6N2E2_3081 0.28 +0.5 3,569,381 - Pf6N2E2_3081 0.28 -1.3 3,569,433 + Pf6N2E2_3081 0.35 -0.8 3,569,433 + Pf6N2E2_3081 0.35 -0.0 3,569,433 + Pf6N2E2_3081 0.35 +0.9 3,569,434 - Pf6N2E2_3081 0.35 +0.7 3,569,434 - Pf6N2E2_3081 0.35 +0.7 3,569,626 + Pf6N2E2_3081 0.58 +0.1 3,569,626 + Pf6N2E2_3081 0.58 -0.5 3,569,626 + Pf6N2E2_3081 0.58 +0.1 3,569,627 - Pf6N2E2_3081 0.58 +0.6 3,569,627 - Pf6N2E2_3081 0.58 +0.6 3,569,918 - +0.4 3,569,955 - +0.2 3,569,989 + +0.4 3,569,989 + -0.7 3,569,990 - +1.3 3,569,990 - +0.8 3,569,990 - +0.3 3,569,991 + -0.6 3,569,992 - +0.5 3,569,992 - +0.6 3,569,992 - -0.6 3,569,992 - -0.7 3,570,100 + Pf6N2E2_3082 0.49 -0.1 3,570,101 - Pf6N2E2_3082 0.50 +0.2 3,570,102 + Pf6N2E2_3082 0.51 +0.9 3,570,102 + Pf6N2E2_3082 0.51 -0.4 3,570,103 - Pf6N2E2_3082 0.51 -0.5 3,570,107 + Pf6N2E2_3082 0.53 -1.2 3,570,107 + Pf6N2E2_3082 0.53 -0.0 3,570,107 + Pf6N2E2_3082 0.53 -1.3 3,570,107 + Pf6N2E2_3082 0.53 -0.2 3,570,141 + Pf6N2E2_3082 0.72 -0.9 3,570,161 + Pf6N2E2_3082 0.83 +0.3 3,570,161 + Pf6N2E2_3082 0.83 +1.2 3,571,525 + Pf6N2E2_3084 0.24 -0.3 3,571,609 + Pf6N2E2_3084 0.34 +0.6 3,571,609 + Pf6N2E2_3084 0.34 -0.2 3,571,609 + Pf6N2E2_3084 0.34 +0.5 3,571,610 - Pf6N2E2_3084 0.35 -0.6 3,571,610 - Pf6N2E2_3084 0.35 -0.2 3,571,612 + Pf6N2E2_3084 0.35 -1.6 3,571,612 + Pf6N2E2_3084 0.35 +1.0 3,571,613 - Pf6N2E2_3084 0.35 -0.4 3,571,613 - Pf6N2E2_3084 0.35 -0.5 3,571,613 - Pf6N2E2_3084 0.35 -0.3 3,571,909 + Pf6N2E2_3084 0.71 +0.1 3,571,909 + Pf6N2E2_3084 0.71 +0.0 3,571,909 + Pf6N2E2_3084 0.71 -0.9 3,571,910 - Pf6N2E2_3084 0.72 +0.4 3,571,910 - Pf6N2E2_3084 0.72 +0.2 3,572,117 - -0.3 3,572,122 + -0.1 3,572,122 + -0.7 3,572,123 - -1.6 3,572,247 - +1.4
Or see this region's nucleotide sequence