Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3053 and Pf6N2E2_3054 are separated by 149 nucleotides Pf6N2E2_3054 and Pf6N2E2_3055 are separated by 194 nucleotides Pf6N2E2_3055 and Pf6N2E2_3056 are separated by 17 nucleotides
Pf6N2E2_3053 - Acetyl-coenzyme A carboxyl transferase alpha chain (EC 6.4.1.2), at 3,536,772 to 3,537,719
_3053
Pf6N2E2_3054 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 3,537,869 to 3,541,390
_3054
Pf6N2E2_3055 - Ribonuclease HII (EC 3.1.26.4), at 3,541,585 to 3,542,217
_3055
Pf6N2E2_3056 - Lipid-A-disaccharide synthase (EC 2.4.1.182), at 3,542,235 to 3,543,365
_3056
Position (kb)
3537
3538
3539
3540
3541
3542 Strain fitness (log2 ratio)
-2
-1
0
1 at 3541.391 kb on - strand at 3541.405 kb on - strand at 3541.449 kb on - strand at 3541.449 kb on - strand at 3541.537 kb on + strand at 3541.628 kb on + strand at 3541.629 kb on - strand at 3541.629 kb on - strand at 3541.749 kb on - strand, within Pf6N2E2_3055 at 3541.882 kb on - strand, within Pf6N2E2_3055 at 3542.036 kb on + strand, within Pf6N2E2_3055 at 3542.221 kb on + strand at 3542.221 kb on + strand at 3542.221 kb on + strand at 3542.222 kb on - strand at 3542.222 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 3,541,391 - +1.7 3,541,405 - -0.1 3,541,449 - +0.7 3,541,449 - -0.2 3,541,537 + +0.5 3,541,628 + -0.2 3,541,629 - +0.1 3,541,629 - +0.3 3,541,749 - Pf6N2E2_3055 0.26 +0.2 3,541,882 - Pf6N2E2_3055 0.47 -0.4 3,542,036 + Pf6N2E2_3055 0.71 -0.3 3,542,221 + -2.0 3,542,221 + +0.6 3,542,221 + -1.2 3,542,222 - -1.1 3,542,222 - -1.2
Or see this region's nucleotide sequence