Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2553

Experiment: RCH2_defined

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2552 and Pf6N2E2_2553 overlap by 8 nucleotidesPf6N2E2_2553 and Pf6N2E2_2554 overlap by 4 nucleotidesPf6N2E2_2554 and Pf6N2E2_2555 overlap by 4 nucleotides Pf6N2E2_2552 - Cytochrome c heme lyase subunit CcmH, at 3,012,666 to 3,013,868 _2552 Pf6N2E2_2553 - Cytochrome c heme lyase subunit CcmL, at 3,013,861 to 3,014,334 _2553 Pf6N2E2_2554 - Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase, at 3,014,331 to 3,014,867 _2554 Pf6N2E2_2555 - Cytochrome c heme lyase subunit CcmF, at 3,014,864 to 3,016,852 _2555 Position (kb) 3013 3014 3015Strain fitness (log2 ratio) -2 -1 0 1at 3013.160 kb on - strand, within Pf6N2E2_2552at 3013.257 kb on - strand, within Pf6N2E2_2552at 3013.390 kb on + strand, within Pf6N2E2_2552at 3013.390 kb on + strand, within Pf6N2E2_2552at 3013.390 kb on + strand, within Pf6N2E2_2552at 3013.390 kb on + strandat 3013.390 kb on + strand, within Pf6N2E2_2552at 3013.391 kb on - strand, within Pf6N2E2_2552at 3013.391 kb on - strand, within Pf6N2E2_2552at 3013.391 kb on - strand, within Pf6N2E2_2552at 3013.391 kb on - strand, within Pf6N2E2_2552at 3013.391 kb on - strand, within Pf6N2E2_2552at 3013.394 kb on + strand, within Pf6N2E2_2552at 3013.408 kb on + strand, within Pf6N2E2_2552at 3013.408 kb on + strand, within Pf6N2E2_2552at 3013.409 kb on - strand, within Pf6N2E2_2552at 3013.541 kb on - strand, within Pf6N2E2_2552at 3013.541 kb on - strand, within Pf6N2E2_2552at 3013.741 kb on + strand, within Pf6N2E2_2552at 3013.742 kb on - strand, within Pf6N2E2_2552at 3014.363 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined
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3,013,160 - Pf6N2E2_2552 0.41 -0.2
3,013,257 - Pf6N2E2_2552 0.49 +0.9
3,013,390 + Pf6N2E2_2552 0.60 -0.3
3,013,390 + Pf6N2E2_2552 0.60 -0.1
3,013,390 + Pf6N2E2_2552 0.60 +0.0
3,013,390 + +0.2
3,013,390 + Pf6N2E2_2552 0.60 -0.0
3,013,391 - Pf6N2E2_2552 0.60 +0.5
3,013,391 - Pf6N2E2_2552 0.60 -0.1
3,013,391 - Pf6N2E2_2552 0.60 -0.6
3,013,391 - Pf6N2E2_2552 0.60 +0.3
3,013,391 - Pf6N2E2_2552 0.60 +0.2
3,013,394 + Pf6N2E2_2552 0.61 +0.0
3,013,408 + Pf6N2E2_2552 0.62 +0.1
3,013,408 + Pf6N2E2_2552 0.62 +1.1
3,013,409 - Pf6N2E2_2552 0.62 +0.2
3,013,541 - Pf6N2E2_2552 0.73 -2.2
3,013,541 - Pf6N2E2_2552 0.73 -2.1
3,013,741 + Pf6N2E2_2552 0.89 -2.5
3,013,742 - Pf6N2E2_2552 0.89 -2.7
3,014,363 - -1.8

Or see this region's nucleotide sequence