Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2285 and Pf6N2E2_2286 are separated by 106 nucleotides Pf6N2E2_2286 and Pf6N2E2_2287 are separated by 234 nucleotides Pf6N2E2_2287 and Pf6N2E2_2288 are separated by 115 nucleotides Pf6N2E2_2288 and Pf6N2E2_2289 are separated by 108 nucleotides
Pf6N2E2_2285 - FIG00954079: hypothetical protein, at 2,703,870 to 2,704,103
_2285
Pf6N2E2_2286 - FIG00953287: hypothetical protein, at 2,704,210 to 2,704,734
_2286
Pf6N2E2_2287 - DNA topoisomerase I (EC 5.99.1.2), at 2,704,969 to 2,707,581
_2287
Pf6N2E2_2288 - FIG00953423: hypothetical protein, at 2,707,697 to 2,707,933
_2288
Pf6N2E2_2289 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 2,708,042 to 2,709,193
_2289
Position (kb)
2704
2705
2706
2707
2708 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2703.990 kb on + strand, within Pf6N2E2_2285 at 2703.991 kb on - strand, within Pf6N2E2_2285 at 2704.086 kb on + strand at 2704.097 kb on + strand at 2704.097 kb on + strand at 2704.098 kb on - strand at 2704.098 kb on - strand at 2704.103 kb on + strand at 2704.104 kb on - strand at 2704.104 kb on - strand at 2704.104 kb on - strand at 2704.104 kb on - strand at 2704.104 kb on - strand at 2704.134 kb on + strand at 2704.134 kb on + strand at 2704.134 kb on + strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.135 kb on - strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.136 kb on + strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.137 kb on - strand at 2704.138 kb on + strand at 2704.138 kb on + strand at 2704.138 kb on + strand at 2704.138 kb on + strand at 2704.139 kb on - strand at 2704.139 kb on - strand at 2704.192 kb on - strand at 2704.545 kb on - strand, within Pf6N2E2_2286 at 2704.586 kb on + strand, within Pf6N2E2_2286 at 2704.586 kb on + strand, within Pf6N2E2_2286 at 2704.587 kb on - strand, within Pf6N2E2_2286 at 2704.694 kb on + strand at 2704.718 kb on + strand at 2704.718 kb on + strand at 2704.718 kb on + strand at 2704.719 kb on - strand at 2704.719 kb on - strand at 2704.719 kb on - strand at 2704.839 kb on - strand at 2705.006 kb on + strand at 2705.007 kb on - strand at 2705.009 kb on + strand at 2705.010 kb on - strand at 2705.054 kb on + strand at 2705.055 kb on - strand at 2705.261 kb on + strand, within Pf6N2E2_2287 at 2705.261 kb on + strand, within Pf6N2E2_2287 at 2705.261 kb on + strand at 2705.262 kb on - strand, within Pf6N2E2_2287 at 2705.262 kb on - strand, within Pf6N2E2_2287 at 2707.644 kb on - strand at 2707.644 kb on - strand at 2707.644 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.646 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.648 kb on - strand at 2707.650 kb on - strand at 2707.650 kb on - strand at 2707.650 kb on - strand at 2707.650 kb on - strand at 2707.833 kb on + strand, within Pf6N2E2_2288 at 2707.880 kb on + strand, within Pf6N2E2_2288 at 2707.902 kb on + strand, within Pf6N2E2_2288 at 2707.902 kb on + strand, within Pf6N2E2_2288 at 2707.903 kb on - strand, within Pf6N2E2_2288 at 2707.903 kb on - strand, within Pf6N2E2_2288 at 2707.903 kb on - strand, within Pf6N2E2_2288 at 2707.903 kb on - strand, within Pf6N2E2_2288 at 2707.911 kb on + strand at 2707.925 kb on + strand at 2707.925 kb on + strand at 2707.926 kb on - strand at 2707.982 kb on - strand at 2707.984 kb on + strand at 2707.985 kb on - strand at 2707.985 kb on - strand at 2708.043 kb on + strand at 2708.044 kb on - strand at 2708.044 kb on - strand at 2708.362 kb on - strand, within Pf6N2E2_2289
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 2,703,990 + Pf6N2E2_2285 0.51 -0.4 2,703,991 - Pf6N2E2_2285 0.52 -0.2 2,704,086 + -1.7 2,704,097 + +1.3 2,704,097 + -0.0 2,704,098 - -0.1 2,704,098 - +0.8 2,704,103 + +1.1 2,704,104 - -1.5 2,704,104 - -0.8 2,704,104 - -0.0 2,704,104 - +1.2 2,704,104 - -0.0 2,704,134 + -0.8 2,704,134 + -1.2 2,704,134 + -1.5 2,704,135 - -0.2 2,704,135 - -0.2 2,704,135 - +0.2 2,704,135 - +1.1 2,704,135 - +1.4 2,704,135 - -0.5 2,704,135 - -0.9 2,704,135 - -0.4 2,704,135 - +0.3 2,704,135 - +0.3 2,704,135 - -0.1 2,704,136 + +0.6 2,704,136 + +1.0 2,704,136 + +0.6 2,704,136 + -0.2 2,704,136 + -1.2 2,704,136 + -2.1 2,704,136 + +0.5 2,704,136 + +0.9 2,704,137 - +0.1 2,704,137 - -0.3 2,704,137 - +0.7 2,704,137 - +1.4 2,704,137 - +0.1 2,704,137 - -0.2 2,704,137 - +0.8 2,704,137 - +0.5 2,704,137 - -0.7 2,704,137 - -0.5 2,704,137 - +0.0 2,704,137 - +1.5 2,704,137 - +0.3 2,704,137 - +0.9 2,704,138 + -0.1 2,704,138 + -0.3 2,704,138 + -1.4 2,704,138 + -1.4 2,704,139 - +0.1 2,704,139 - +0.1 2,704,192 - -0.3 2,704,545 - Pf6N2E2_2286 0.64 -0.5 2,704,586 + Pf6N2E2_2286 0.72 -0.7 2,704,586 + Pf6N2E2_2286 0.72 -3.1 2,704,587 - Pf6N2E2_2286 0.72 -0.4 2,704,694 + -0.5 2,704,718 + +1.3 2,704,718 + -1.4 2,704,718 + -1.7 2,704,719 - -0.8 2,704,719 - -1.2 2,704,719 - -3.0 2,704,839 - +0.2 2,705,006 + -2.8 2,705,007 - +0.4 2,705,009 + -2.6 2,705,010 - +0.5 2,705,054 + -1.9 2,705,055 - -1.2 2,705,261 + Pf6N2E2_2287 0.11 -0.3 2,705,261 + Pf6N2E2_2287 0.11 -1.4 2,705,261 + -1.2 2,705,262 - Pf6N2E2_2287 0.11 +0.1 2,705,262 - Pf6N2E2_2287 0.11 -2.1 2,707,644 - -0.9 2,707,644 - +0.9 2,707,644 - +0.2 2,707,646 - -1.8 2,707,646 - -0.2 2,707,646 - +0.1 2,707,646 - +0.2 2,707,646 - -0.6 2,707,646 - +1.1 2,707,646 - +0.2 2,707,646 - +0.2 2,707,646 - -0.3 2,707,646 - -0.8 2,707,646 - -0.4 2,707,646 - -0.4 2,707,646 - -0.0 2,707,646 - -0.4 2,707,646 - +0.2 2,707,646 - -0.3 2,707,646 - -0.6 2,707,646 - +0.4 2,707,646 - +0.1 2,707,646 - -0.0 2,707,648 - -0.4 2,707,648 - +0.5 2,707,648 - -0.3 2,707,648 - +0.2 2,707,648 - +0.7 2,707,648 - -0.0 2,707,648 - +0.8 2,707,648 - +0.6 2,707,648 - +0.4 2,707,648 - -0.4 2,707,648 - -0.3 2,707,648 - -0.4 2,707,648 - -0.4 2,707,648 - -0.5 2,707,648 - +0.1 2,707,650 - -0.4 2,707,650 - +0.8 2,707,650 - -0.1 2,707,650 - -0.4 2,707,833 + Pf6N2E2_2288 0.57 +0.4 2,707,880 + Pf6N2E2_2288 0.77 +0.4 2,707,902 + Pf6N2E2_2288 0.86 +0.2 2,707,902 + Pf6N2E2_2288 0.86 +0.6 2,707,903 - Pf6N2E2_2288 0.87 +0.3 2,707,903 - Pf6N2E2_2288 0.87 +0.8 2,707,903 - Pf6N2E2_2288 0.87 -0.3 2,707,903 - Pf6N2E2_2288 0.87 +0.7 2,707,911 + -0.1 2,707,925 + -0.6 2,707,925 + -0.2 2,707,926 - -0.3 2,707,982 - -0.1 2,707,984 + -0.3 2,707,985 - -0.3 2,707,985 - -0.2 2,708,043 + -0.9 2,708,044 - -0.7 2,708,044 - +0.4 2,708,362 - Pf6N2E2_2289 0.28 +0.5
Or see this region's nucleotide sequence