Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1837 and Pf6N2E2_1838 are separated by 10 nucleotides Pf6N2E2_1838 and Pf6N2E2_1839 overlap by 1 nucleotides Pf6N2E2_1839 and Pf6N2E2_1840 overlap by 4 nucleotides Pf6N2E2_1840 and Pf6N2E2_1841 are separated by 10 nucleotides
Pf6N2E2_1837 - Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10), at 2,175,429 to 2,176,907
_1837
Pf6N2E2_1838 - 6-hexanolactone hydrolase, at 2,176,918 to 2,177,823
_1838
Pf6N2E2_1839 - 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (EC 1.1.1.-), at 2,177,823 to 2,178,557
_1839
Pf6N2E2_1840 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,178,554 to 2,179,366
_1840
Pf6N2E2_1841 - hypothetical protein, at 2,179,377 to 2,179,526
_1841
Position (kb)
2177
2178
2179 Strain fitness (log2 ratio)
-1
0
1
2 at 2176.877 kb on - strand at 2176.924 kb on + strand at 2176.925 kb on - strand at 2177.029 kb on + strand, within Pf6N2E2_1838 at 2177.201 kb on + strand, within Pf6N2E2_1838 at 2177.267 kb on + strand, within Pf6N2E2_1838 at 2177.456 kb on - strand, within Pf6N2E2_1838 at 2177.456 kb on - strand, within Pf6N2E2_1838 at 2177.603 kb on + strand, within Pf6N2E2_1838 at 2177.792 kb on - strand at 2178.115 kb on + strand, within Pf6N2E2_1839 at 2178.225 kb on + strand, within Pf6N2E2_1839 at 2178.254 kb on - strand, within Pf6N2E2_1839 at 2178.254 kb on - strand, within Pf6N2E2_1839 at 2178.302 kb on - strand, within Pf6N2E2_1839 at 2178.435 kb on - strand, within Pf6N2E2_1839 at 2178.500 kb on + strand at 2178.707 kb on - strand, within Pf6N2E2_1840 at 2179.087 kb on - strand, within Pf6N2E2_1840 at 2179.087 kb on - strand, within Pf6N2E2_1840 at 2179.105 kb on + strand, within Pf6N2E2_1840 at 2179.107 kb on + strand, within Pf6N2E2_1840 at 2179.108 kb on - strand, within Pf6N2E2_1840 at 2179.108 kb on - strand, within Pf6N2E2_1840 at 2179.141 kb on + strand, within Pf6N2E2_1840 at 2179.141 kb on + strand, within Pf6N2E2_1840 at 2179.142 kb on - strand, within Pf6N2E2_1840
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 2,176,877 - +0.2 2,176,924 + +0.5 2,176,925 - +0.1 2,177,029 + Pf6N2E2_1838 0.12 +0.7 2,177,201 + Pf6N2E2_1838 0.31 +0.9 2,177,267 + Pf6N2E2_1838 0.39 -0.9 2,177,456 - Pf6N2E2_1838 0.59 -0.7 2,177,456 - Pf6N2E2_1838 0.59 +0.2 2,177,603 + Pf6N2E2_1838 0.76 +0.5 2,177,792 - +0.3 2,178,115 + Pf6N2E2_1839 0.40 -0.5 2,178,225 + Pf6N2E2_1839 0.55 -0.2 2,178,254 - Pf6N2E2_1839 0.59 +0.1 2,178,254 - Pf6N2E2_1839 0.59 -0.7 2,178,302 - Pf6N2E2_1839 0.65 -1.5 2,178,435 - Pf6N2E2_1839 0.83 -0.7 2,178,500 + -0.4 2,178,707 - Pf6N2E2_1840 0.19 +0.2 2,179,087 - Pf6N2E2_1840 0.66 +0.2 2,179,087 - Pf6N2E2_1840 0.66 +1.0 2,179,105 + Pf6N2E2_1840 0.68 -0.6 2,179,107 + Pf6N2E2_1840 0.68 -1.2 2,179,108 - Pf6N2E2_1840 0.68 -0.2 2,179,108 - Pf6N2E2_1840 0.68 +2.2 2,179,141 + Pf6N2E2_1840 0.72 +0.3 2,179,141 + Pf6N2E2_1840 0.72 -0.6 2,179,142 - Pf6N2E2_1840 0.72 +1.6
Or see this region's nucleotide sequence