Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_177

Experiment: RCH2_defined

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_174 and Pf6N2E2_175 are separated by 60 nucleotidesPf6N2E2_175 and Pf6N2E2_176 are separated by 30 nucleotidesPf6N2E2_176 and Pf6N2E2_177 are separated by 109 nucleotidesPf6N2E2_177 and Pf6N2E2_178 are separated by 35 nucleotides Pf6N2E2_174 - Translation initiation factor 3, at 225,586 to 226,119 _174 Pf6N2E2_175 - LSU ribosomal protein L35p, at 226,180 to 226,374 _175 Pf6N2E2_176 - LSU ribosomal protein L20p, at 226,405 to 226,761 _176 Pf6N2E2_177 - Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20), at 226,871 to 227,887 _177 Pf6N2E2_178 - Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20), at 227,923 to 230,301 _178 Position (kb) 226 227 228Strain fitness (log2 ratio) -1 0 1at 226.117 kb on + strandat 226.855 kb on + strandat 226.855 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined
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226,117 + -1.2
226,855 + +0.9
226,855 + +0.1

Or see this region's nucleotide sequence