Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT0543

Experiment: Varel_Bryant_medium_Glucose with Zinc Pyrithione 0.05 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT0541 and BT0542 are separated by 172 nucleotidesBT0542 and BT0543 are separated by 121 nucleotidesBT0543 and BT0544 are separated by 55 nucleotides BT0541: BT0541 - Polyphosphate kinase (NCBI ptt file), at 664,997 to 667,114 BT0541 BT0542: BT0542 - conserved hypothetical protein (NCBI ptt file), at 667,287 to 667,865 BT0542 BT0543: BT0543 - glutamine synthetase (NCBI ptt file), at 667,987 to 670,176 BT0543 BT0544: BT0544 - ammonium transporter (NCBI ptt file), at 670,232 to 671,689 BT0544 Position (kb) 667 668 669 670 671Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 667.011 kb on - strandat 667.220 kb on + strandat 667.228 kb on + strandat 667.239 kb on - strandat 667.284 kb on + strandat 667.302 kb on + strandat 667.377 kb on + strand, within BT0542at 667.575 kb on - strand, within BT0542at 667.718 kb on + strand, within BT0542at 667.718 kb on + strand, within BT0542at 667.719 kb on - strand, within BT0542at 667.719 kb on - strand, within BT0542at 667.839 kb on - strandat 667.858 kb on + strandat 667.947 kb on + strandat 667.947 kb on + strandat 667.964 kb on - strandat 667.994 kb on - strandat 668.065 kb on - strandat 668.065 kb on - strandat 668.101 kb on - strandat 668.166 kb on - strandat 668.176 kb on + strandat 668.292 kb on + strand, within BT0543at 668.293 kb on - strand, within BT0543at 668.293 kb on - strand, within BT0543at 668.293 kb on - strand, within BT0543at 668.332 kb on - strand, within BT0543at 668.461 kb on - strand, within BT0543at 668.483 kb on + strand, within BT0543at 668.543 kb on - strand, within BT0543at 668.614 kb on + strand, within BT0543at 668.811 kb on + strand, within BT0543at 669.061 kb on + strand, within BT0543at 669.147 kb on + strand, within BT0543at 669.417 kb on - strand, within BT0543at 669.482 kb on - strand, within BT0543at 669.661 kb on - strand, within BT0543at 669.662 kb on + strand, within BT0543at 669.663 kb on - strand, within BT0543at 669.806 kb on - strand, within BT0543at 669.806 kb on - strand, within BT0543at 670.009 kb on - strandat 670.029 kb on - strandat 670.077 kb on - strandat 670.080 kb on + strandat 670.080 kb on + strandat 670.150 kb on + strandat 670.176 kb on + strandat 670.176 kb on + strandat 670.199 kb on + strandat 670.239 kb on + strandat 670.239 kb on + strandat 670.239 kb on + strandat 670.521 kb on - strand, within BT0544at 670.531 kb on + strand, within BT0544at 670.532 kb on - strand, within BT0544at 670.557 kb on - strand, within BT0544at 670.599 kb on - strand, within BT0544at 670.835 kb on + strand, within BT0544at 670.924 kb on + strand, within BT0544at 670.925 kb on - strand, within BT0544at 671.099 kb on - strand, within BT0544at 671.104 kb on + strand, within BT0544at 671.105 kb on - strand, within BT0544at 671.107 kb on + strand, within BT0544at 671.110 kb on - strand, within BT0544at 671.118 kb on + strand, within BT0544

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Per-strain Table

Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Zinc Pyrithione 0.05 mM
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667,011 - -1.6
667,220 + -0.3
667,228 + -1.0
667,239 - -1.6
667,284 + -1.6
667,302 + -0.0
667,377 + BT0542 0.16 -1.0
667,575 - BT0542 0.50 -3.0
667,718 + BT0542 0.74 -0.0
667,718 + BT0542 0.74 -0.0
667,719 - BT0542 0.75 -0.0
667,719 - BT0542 0.75 +0.4
667,839 - +0.3
667,858 + -1.6
667,947 + -0.2
667,947 + +0.5
667,964 - -1.0
667,994 - +0.1
668,065 - -0.3
668,065 - -0.0
668,101 - -2.6
668,166 - +1.4
668,176 + -0.0
668,292 + BT0543 0.14 -1.0
668,293 - BT0543 0.14 +0.8
668,293 - BT0543 0.14 -2.4
668,293 - BT0543 0.14 -1.6
668,332 - BT0543 0.16 -1.6
668,461 - BT0543 0.22 +1.8
668,483 + BT0543 0.23 -0.0
668,543 - BT0543 0.25 -1.0
668,614 + BT0543 0.29 -0.0
668,811 + BT0543 0.38 -1.0
669,061 + BT0543 0.49 -3.2
669,147 + BT0543 0.53 -0.0
669,417 - BT0543 0.65 -0.0
669,482 - BT0543 0.68 -0.0
669,661 - BT0543 0.76 -0.0
669,662 + BT0543 0.76 -0.5
669,663 - BT0543 0.77 +1.0
669,806 - BT0543 0.83 -0.0
669,806 - BT0543 0.83 -2.4
670,009 - -1.5
670,029 - -0.0
670,077 - -1.0
670,080 + -1.6
670,080 + +1.3
670,150 + +0.5
670,176 + -1.0
670,176 + -0.0
670,199 + -4.6
670,239 + -3.9
670,239 + -1.6
670,239 + -0.8
670,521 - BT0544 0.20 -0.6
670,531 + BT0544 0.21 -1.0
670,532 - BT0544 0.21 -2.4
670,557 - BT0544 0.22 -2.6
670,599 - BT0544 0.25 -1.7
670,835 + BT0544 0.41 -1.0
670,924 + BT0544 0.47 -0.0
670,925 - BT0544 0.48 +0.7
671,099 - BT0544 0.59 -2.0
671,104 + BT0544 0.60 +0.4
671,105 - BT0544 0.60 -1.0
671,107 + BT0544 0.60 +0.2
671,110 - BT0544 0.60 -1.6
671,118 + BT0544 0.61 -0.0

Or see this region's nucleotide sequence