Experiment: MoLS4 with L-Tryptophan as nutrient source 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt divIVA and DVU1374 overlap by 8 nucleotides DVU1374 and DVU1375 are separated by 20 nucleotides DVU1375 and ilvB-2 are separated by 0 nucleotides ilvB-2 and ilvN-2 are separated by 9 nucleotides ilvN-2 and ilvC are separated by 121 nucleotides
DVU1373: divIVA - cell-division initiation protein DivIVA (TIGR), at 1,450,470 to 1,450,979
divIVA
DVU1374: DVU1374 - conserved hypothetical protein (TIGR), at 1,450,972 to 1,451,292
DVU1374
DVU1375: DVU1375 - hypothetical protein (TIGR), at 1,451,313 to 1,451,543
DVU1375
DVU1376: ilvB-2 - acetolactate synthase, large subunit, biosynthetic type (TIGR), at 1,451,544 to 1,453,235
ilvB-2
DVU1377: ilvN-2 - acetolactate synthase, small subunit (TIGR), at 1,453,245 to 1,453,733
ilvN-2
DVU1378: ilvC - ketol-acid reductoisomerase (TIGR), at 1,453,855 to 1,454,850
ilvC
Position (kb) 1451
1452
1453
1454 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1450.687 kb on - strand, within divIVA at 1450.689 kb on + strand, within divIVA at 1450.992 kb on + strand at 1451.000 kb on - strand at 1451.060 kb on + strand, within DVU1374 at 1451.084 kb on + strand, within DVU1374 at 1451.097 kb on + strand, within DVU1374 at 1451.126 kb on - strand, within DVU1374 at 1451.172 kb on + strand, within DVU1374 at 1451.262 kb on + strand at 1451.352 kb on + strand, within DVU1375 at 1451.352 kb on + strand, within DVU1375 at 1451.360 kb on - strand, within DVU1375 at 1451.362 kb on - strand, within DVU1375 at 1451.522 kb on + strand at 1451.530 kb on - strand at 1451.530 kb on - strand at 1451.537 kb on + strand at 1451.567 kb on - strand at 1451.723 kb on + strand, within ilvB-2 at 1451.730 kb on + strand, within ilvB-2 at 1451.738 kb on - strand, within ilvB-2 at 1451.740 kb on + strand, within ilvB-2 at 1451.813 kb on - strand, within ilvB-2 at 1451.831 kb on + strand, within ilvB-2 at 1451.924 kb on + strand, within ilvB-2 at 1451.924 kb on + strand, within ilvB-2 at 1451.932 kb on - strand, within ilvB-2 at 1451.932 kb on - strand, within ilvB-2 at 1452.056 kb on + strand, within ilvB-2 at 1452.143 kb on + strand, within ilvB-2 at 1452.240 kb on + strand, within ilvB-2 at 1452.568 kb on - strand, within ilvB-2 at 1452.568 kb on - strand, within ilvB-2 at 1452.568 kb on - strand, within ilvB-2 at 1452.568 kb on - strand, within ilvB-2 at 1452.717 kb on - strand, within ilvB-2 at 1452.746 kb on + strand, within ilvB-2 at 1452.746 kb on + strand, within ilvB-2 at 1452.746 kb on + strand, within ilvB-2 at 1452.746 kb on + strand, within ilvB-2 at 1452.746 kb on + strand, within ilvB-2 at 1453.110 kb on - strand at 1453.158 kb on - strand at 1453.247 kb on - strand at 1453.249 kb on + strand at 1453.376 kb on + strand, within ilvN-2 at 1453.486 kb on + strand, within ilvN-2 at 1453.696 kb on + strand at 1453.701 kb on + strand at 1454.150 kb on - strand, within ilvC at 1454.208 kb on + strand, within ilvC
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Tryptophan as nutrient source 10mM remove 1,450,687 - divIVA DVU1373 0.43 -1.0 1,450,689 + divIVA DVU1373 0.43 -0.4 1,450,992 + +0.1 1,451,000 - -1.0 1,451,060 + DVU1374 0.27 -0.4 1,451,084 + DVU1374 0.35 -0.4 1,451,097 + DVU1374 0.39 +0.1 1,451,126 - DVU1374 0.48 +0.4 1,451,172 + DVU1374 0.62 -0.2 1,451,262 + -0.2 1,451,352 + DVU1375 0.17 +0.5 1,451,352 + DVU1375 0.17 -0.1 1,451,360 - DVU1375 0.20 +0.0 1,451,362 - DVU1375 0.21 -0.8 1,451,522 + -2.6 1,451,530 - -0.9 1,451,530 - -0.7 1,451,537 + -2.1 1,451,567 - -2.0 1,451,723 + ilvB-2 DVU1376 0.11 -0.5 1,451,730 + ilvB-2 DVU1376 0.11 -4.1 1,451,738 - ilvB-2 DVU1376 0.11 -3.8 1,451,740 + ilvB-2 DVU1376 0.12 -4.6 1,451,813 - ilvB-2 DVU1376 0.16 -1.2 1,451,831 + ilvB-2 DVU1376 0.17 -2.9 1,451,924 + ilvB-2 DVU1376 0.22 -3.7 1,451,924 + ilvB-2 DVU1376 0.22 -3.1 1,451,932 - ilvB-2 DVU1376 0.23 -3.1 1,451,932 - ilvB-2 DVU1376 0.23 -5.7 1,452,056 + ilvB-2 DVU1376 0.30 -2.9 1,452,143 + ilvB-2 DVU1376 0.35 -2.6 1,452,240 + ilvB-2 DVU1376 0.41 +0.9 1,452,568 - ilvB-2 DVU1376 0.61 -1.5 1,452,568 - ilvB-2 DVU1376 0.61 -4.1 1,452,568 - ilvB-2 DVU1376 0.61 -4.6 1,452,568 - ilvB-2 DVU1376 0.61 -4.5 1,452,717 - ilvB-2 DVU1376 0.69 -1.1 1,452,746 + ilvB-2 DVU1376 0.71 -1.2 1,452,746 + ilvB-2 DVU1376 0.71 -0.4 1,452,746 + ilvB-2 DVU1376 0.71 -0.1 1,452,746 + ilvB-2 DVU1376 0.71 -2.5 1,452,746 + ilvB-2 DVU1376 0.71 -2.4 1,453,110 - -4.5 1,453,158 - -3.4 1,453,247 - -0.2 1,453,249 + -2.4 1,453,376 + ilvN-2 DVU1377 0.27 -0.2 1,453,486 + ilvN-2 DVU1377 0.49 -0.5 1,453,696 + -0.4 1,453,701 + -1.9 1,454,150 - ilvC DVU1378 0.30 -4.7 1,454,208 + ilvC DVU1378 0.35 -4.6
Or see this region's nucleotide sequence