Experiment: KB with Avocadene 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3972 and Psyr_3973 are separated by 27 nucleotides Psyr_3973 and Psyr_3974 overlap by 4 nucleotides Psyr_3974 and Psyr_3975 overlap by 4 nucleotides Psyr_3975 and Psyr_3976 are separated by 11 nucleotides
Psyr_3972: Psyr_3972 - ABC transporter, periplasmic substrate-binding protein, at 4,719,943 to 4,721,010
_3972
Psyr_3973: Psyr_3973 - Binding-protein-dependent transport systems inner membrane component, at 4,721,038 to 4,721,853
_3973
Psyr_3974: Psyr_3974 - ABC transporter, at 4,721,850 to 4,722,632
_3974
Psyr_3975: Psyr_3975 - conserved hypothetical protein, at 4,722,629 to 4,723,354
_3975
Psyr_3976: Psyr_3976 - conserved hypothetical protein, at 4,723,366 to 4,723,983
_3976
Position (kb)
4721
4722
4723 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4720.974 kb on + strand at 4720.974 kb on + strand at 4720.974 kb on + strand at 4720.975 kb on - strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.978 kb on + strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4720.979 kb on - strand at 4721.008 kb on + strand at 4721.008 kb on + strand at 4721.009 kb on - strand at 4721.009 kb on - strand at 4721.022 kb on + strand at 4721.023 kb on - strand at 4721.081 kb on + strand at 4721.120 kb on - strand, within Psyr_3973 at 4721.120 kb on - strand, within Psyr_3973 at 4721.243 kb on - strand, within Psyr_3973 at 4721.729 kb on - strand, within Psyr_3973 at 4721.755 kb on + strand, within Psyr_3973 at 4721.755 kb on + strand, within Psyr_3973 at 4721.755 kb on + strand, within Psyr_3973 at 4721.755 kb on + strand, within Psyr_3973 at 4721.755 kb on + strand, within Psyr_3973 at 4721.756 kb on - strand, within Psyr_3973 at 4721.756 kb on - strand, within Psyr_3973 at 4721.756 kb on - strand, within Psyr_3973 at 4721.887 kb on - strand at 4722.258 kb on + strand, within Psyr_3974 at 4722.499 kb on + strand, within Psyr_3974 at 4722.500 kb on - strand, within Psyr_3974 at 4722.500 kb on - strand, within Psyr_3974 at 4722.546 kb on + strand, within Psyr_3974 at 4722.546 kb on + strand, within Psyr_3974 at 4722.547 kb on - strand, within Psyr_3974 at 4722.547 kb on - strand, within Psyr_3974 at 4722.547 kb on - strand, within Psyr_3974 at 4722.547 kb on - strand, within Psyr_3974 at 4722.705 kb on + strand, within Psyr_3975 at 4722.706 kb on - strand, within Psyr_3975 at 4722.860 kb on + strand, within Psyr_3975 at 4722.861 kb on - strand, within Psyr_3975 at 4722.902 kb on - strand, within Psyr_3975 at 4722.902 kb on - strand, within Psyr_3975 at 4722.963 kb on - strand, within Psyr_3975 at 4723.154 kb on + strand, within Psyr_3975 at 4723.154 kb on + strand, within Psyr_3975 at 4723.154 kb on + strand, within Psyr_3975 at 4723.154 kb on + strand, within Psyr_3975 at 4723.155 kb on - strand, within Psyr_3975 at 4723.155 kb on - strand, within Psyr_3975 at 4723.155 kb on - strand, within Psyr_3975 at 4723.155 kb on - strand, within Psyr_3975 at 4723.155 kb on - strand, within Psyr_3975 at 4723.167 kb on - strand, within Psyr_3975 at 4723.233 kb on - strand, within Psyr_3975 at 4723.565 kb on - strand, within Psyr_3976 at 4723.565 kb on - strand, within Psyr_3976
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocadene 10 mM remove 4,720,974 + +0.4 4,720,974 + +0.2 4,720,974 + +0.9 4,720,975 - +0.2 4,720,978 + +0.7 4,720,978 + -1.5 4,720,978 + +0.3 4,720,978 + +0.1 4,720,978 + +1.1 4,720,978 + +0.6 4,720,978 + -0.4 4,720,978 + -0.5 4,720,978 + +0.6 4,720,978 + +0.1 4,720,979 - +0.5 4,720,979 - -1.6 4,720,979 - +0.1 4,720,979 - -0.3 4,720,979 - +0.1 4,720,979 - -1.1 4,720,979 - +0.9 4,720,979 - +0.1 4,720,979 - +0.5 4,721,008 + +0.3 4,721,008 + +0.4 4,721,009 - -0.4 4,721,009 - -0.7 4,721,022 + +0.0 4,721,023 - -1.2 4,721,081 + +0.2 4,721,120 - Psyr_3973 0.10 -0.3 4,721,120 - Psyr_3973 0.10 -0.2 4,721,243 - Psyr_3973 0.25 +0.3 4,721,729 - Psyr_3973 0.85 -0.1 4,721,755 + Psyr_3973 0.88 -1.1 4,721,755 + Psyr_3973 0.88 +0.3 4,721,755 + Psyr_3973 0.88 +0.1 4,721,755 + Psyr_3973 0.88 +1.2 4,721,755 + Psyr_3973 0.88 +1.4 4,721,756 - Psyr_3973 0.88 +1.8 4,721,756 - Psyr_3973 0.88 +0.1 4,721,756 - Psyr_3973 0.88 +0.9 4,721,887 - +0.6 4,722,258 + Psyr_3974 0.52 -1.2 4,722,499 + Psyr_3974 0.83 -0.6 4,722,500 - Psyr_3974 0.83 -2.9 4,722,500 - Psyr_3974 0.83 -0.2 4,722,546 + Psyr_3974 0.89 +0.2 4,722,546 + Psyr_3974 0.89 +0.2 4,722,547 - Psyr_3974 0.89 -0.2 4,722,547 - Psyr_3974 0.89 -1.2 4,722,547 - Psyr_3974 0.89 -0.1 4,722,547 - Psyr_3974 0.89 -1.0 4,722,705 + Psyr_3975 0.10 +0.6 4,722,706 - Psyr_3975 0.11 -0.2 4,722,860 + Psyr_3975 0.32 +0.7 4,722,861 - Psyr_3975 0.32 +0.7 4,722,902 - Psyr_3975 0.38 +0.5 4,722,902 - Psyr_3975 0.38 +1.4 4,722,963 - Psyr_3975 0.46 -0.3 4,723,154 + Psyr_3975 0.72 -0.4 4,723,154 + Psyr_3975 0.72 +0.4 4,723,154 + Psyr_3975 0.72 -0.4 4,723,154 + Psyr_3975 0.72 -0.2 4,723,155 - Psyr_3975 0.72 -0.7 4,723,155 - Psyr_3975 0.72 -0.2 4,723,155 - Psyr_3975 0.72 +0.2 4,723,155 - Psyr_3975 0.72 +0.3 4,723,155 - Psyr_3975 0.72 -0.7 4,723,167 - Psyr_3975 0.74 -1.2 4,723,233 - Psyr_3975 0.83 -0.1 4,723,565 - Psyr_3976 0.32 -0.7 4,723,565 - Psyr_3976 0.32 +0.4
Or see this region's nucleotide sequence