Experiment: KB with Brucine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2239 and Psyr_2240 are separated by 205 nucleotides Psyr_2240 and Psyr_2241 are separated by 162 nucleotides
Psyr_2239: Psyr_2239 - DNA/RNA non-specific endonuclease, at 2,602,454 to 2,603,398
_2239
Psyr_2240: Psyr_2240 - Histidine kinase, HAMP region:Bacterial chemotaxis sensory transducer, at 2,603,604 to 2,605,217
_2240
Psyr_2241: Psyr_2241 - methyl-accepting chemotaxis sensory transducer, at 2,605,380 to 2,607,176
_2241
Position (kb)
2603
2604
2605
2606 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2602.662 kb on + strand, within Psyr_2239 at 2602.662 kb on + strand, within Psyr_2239 at 2602.662 kb on + strand, within Psyr_2239 at 2602.662 kb on + strand, within Psyr_2239 at 2602.662 kb on + strand, within Psyr_2239 at 2602.662 kb on + strand, within Psyr_2239 at 2602.663 kb on - strand, within Psyr_2239 at 2602.663 kb on - strand at 2602.824 kb on + strand, within Psyr_2239 at 2602.824 kb on + strand, within Psyr_2239 at 2602.825 kb on - strand, within Psyr_2239 at 2602.825 kb on - strand, within Psyr_2239 at 2602.825 kb on - strand, within Psyr_2239 at 2602.933 kb on + strand, within Psyr_2239 at 2602.984 kb on - strand, within Psyr_2239 at 2602.984 kb on - strand, within Psyr_2239 at 2602.984 kb on - strand, within Psyr_2239 at 2602.984 kb on - strand, within Psyr_2239 at 2603.060 kb on + strand, within Psyr_2239 at 2603.061 kb on - strand, within Psyr_2239 at 2603.061 kb on - strand, within Psyr_2239 at 2603.094 kb on + strand, within Psyr_2239 at 2603.095 kb on - strand, within Psyr_2239 at 2603.143 kb on - strand at 2603.143 kb on - strand, within Psyr_2239 at 2603.431 kb on + strand at 2603.431 kb on + strand at 2603.432 kb on - strand at 2603.442 kb on - strand at 2603.514 kb on + strand at 2603.514 kb on + strand at 2603.515 kb on - strand at 2603.520 kb on + strand at 2603.520 kb on + strand at 2603.521 kb on - strand at 2603.521 kb on - strand at 2603.521 kb on - strand at 2603.521 kb on - strand at 2603.521 kb on - strand at 2603.521 kb on - strand at 2603.524 kb on + strand at 2603.525 kb on - strand at 2603.555 kb on + strand at 2603.617 kb on + strand at 2603.618 kb on - strand at 2603.618 kb on - strand at 2603.635 kb on + strand at 2603.648 kb on - strand at 2603.679 kb on + strand at 2603.680 kb on - strand at 2603.680 kb on - strand at 2603.967 kb on + strand, within Psyr_2240 at 2603.967 kb on + strand, within Psyr_2240 at 2603.967 kb on + strand, within Psyr_2240 at 2603.967 kb on + strand, within Psyr_2240 at 2603.968 kb on - strand, within Psyr_2240 at 2603.968 kb on - strand, within Psyr_2240 at 2603.968 kb on - strand, within Psyr_2240 at 2603.968 kb on - strand, within Psyr_2240 at 2603.969 kb on + strand, within Psyr_2240 at 2603.969 kb on + strand, within Psyr_2240 at 2603.969 kb on + strand, within Psyr_2240 at 2603.970 kb on - strand, within Psyr_2240 at 2604.185 kb on + strand, within Psyr_2240 at 2604.186 kb on - strand, within Psyr_2240 at 2604.525 kb on - strand, within Psyr_2240 at 2604.710 kb on + strand, within Psyr_2240 at 2604.713 kb on + strand, within Psyr_2240 at 2605.007 kb on + strand, within Psyr_2240 at 2605.007 kb on + strand, within Psyr_2240 at 2605.007 kb on + strand, within Psyr_2240 at 2605.008 kb on - strand, within Psyr_2240 at 2605.093 kb on + strand at 2605.093 kb on + strand at 2605.241 kb on + strand at 2605.242 kb on - strand at 2605.390 kb on + strand at 2605.401 kb on + strand at 2605.488 kb on + strand at 2605.506 kb on - strand at 2605.653 kb on + strand, within Psyr_2241 at 2605.653 kb on + strand, within Psyr_2241 at 2605.653 kb on + strand, within Psyr_2241 at 2605.654 kb on - strand, within Psyr_2241 at 2606.165 kb on + strand, within Psyr_2241
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Brucine 10 mM remove 2,602,662 + Psyr_2239 0.22 +0.7 2,602,662 + Psyr_2239 0.22 -0.2 2,602,662 + Psyr_2239 0.22 +0.0 2,602,662 + Psyr_2239 0.22 +0.1 2,602,662 + Psyr_2239 0.22 +1.5 2,602,662 + Psyr_2239 0.22 +0.6 2,602,663 - Psyr_2239 0.22 -0.5 2,602,663 - +2.2 2,602,824 + Psyr_2239 0.39 -0.5 2,602,824 + Psyr_2239 0.39 -1.9 2,602,825 - Psyr_2239 0.39 -0.2 2,602,825 - Psyr_2239 0.39 -0.7 2,602,825 - Psyr_2239 0.39 -1.0 2,602,933 + Psyr_2239 0.51 -0.5 2,602,984 - Psyr_2239 0.56 -0.4 2,602,984 - Psyr_2239 0.56 -0.5 2,602,984 - Psyr_2239 0.56 -1.0 2,602,984 - Psyr_2239 0.56 -0.1 2,603,060 + Psyr_2239 0.64 -0.4 2,603,061 - Psyr_2239 0.64 -2.9 2,603,061 - Psyr_2239 0.64 -0.7 2,603,094 + Psyr_2239 0.68 +0.8 2,603,095 - Psyr_2239 0.68 +0.5 2,603,143 - +1.2 2,603,143 - Psyr_2239 0.73 +0.2 2,603,431 + -0.4 2,603,431 + -0.9 2,603,432 - -0.2 2,603,442 - +0.3 2,603,514 + +0.5 2,603,514 + +0.5 2,603,515 - +0.8 2,603,520 + -0.2 2,603,520 + -0.6 2,603,521 - +1.6 2,603,521 - -0.3 2,603,521 - -0.8 2,603,521 - +0.5 2,603,521 - -1.0 2,603,521 - -1.2 2,603,524 + +1.8 2,603,525 - -1.8 2,603,555 + +0.9 2,603,617 + +0.8 2,603,618 - +0.8 2,603,618 - +0.0 2,603,635 + +0.2 2,603,648 - -2.4 2,603,679 + +1.7 2,603,680 - -1.1 2,603,680 - +0.2 2,603,967 + Psyr_2240 0.22 -0.7 2,603,967 + Psyr_2240 0.22 +2.2 2,603,967 + Psyr_2240 0.22 -1.3 2,603,967 + Psyr_2240 0.22 -1.2 2,603,968 - Psyr_2240 0.23 -0.8 2,603,968 - Psyr_2240 0.23 -2.4 2,603,968 - Psyr_2240 0.23 -0.9 2,603,968 - Psyr_2240 0.23 -0.1 2,603,969 + Psyr_2240 0.23 -1.3 2,603,969 + Psyr_2240 0.23 -1.8 2,603,969 + Psyr_2240 0.23 -0.4 2,603,970 - Psyr_2240 0.23 -0.7 2,604,185 + Psyr_2240 0.36 -1.2 2,604,186 - Psyr_2240 0.36 +0.4 2,604,525 - Psyr_2240 0.57 -0.4 2,604,710 + Psyr_2240 0.69 -0.4 2,604,713 + Psyr_2240 0.69 +2.0 2,605,007 + Psyr_2240 0.87 -1.1 2,605,007 + Psyr_2240 0.87 -1.4 2,605,007 + Psyr_2240 0.87 +1.6 2,605,008 - Psyr_2240 0.87 +0.2 2,605,093 + -0.2 2,605,093 + +0.4 2,605,241 + +0.3 2,605,242 - +0.2 2,605,390 + +1.2 2,605,401 + +0.8 2,605,488 + +0.9 2,605,506 - -0.5 2,605,653 + Psyr_2241 0.15 +1.2 2,605,653 + Psyr_2241 0.15 -1.0 2,605,653 + Psyr_2241 0.15 -1.0 2,605,654 - Psyr_2241 0.15 -1.3 2,606,165 + Psyr_2241 0.44 -0.3
Or see this region's nucleotide sequence