Experiment: KB with Brucine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1714 and Psyr_1715 overlap by 4 nucleotides Psyr_1715 and Psyr_1716 are separated by 42 nucleotides
Psyr_1714: Psyr_1714 - exonuclease SbcC, at 1,939,647 to 1,943,291
_1714
Psyr_1715: Psyr_1715 - Exodeoxyribonuclease I subunit D, at 1,943,288 to 1,944,532
_1715
Psyr_1716: Psyr_1716 - conserved hypothetical protein, at 1,944,575 to 1,946,233
_1716
Position (kb) 1943
1944
1945 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1942.673 kb on + strand, within Psyr_1714 at 1942.673 kb on + strand, within Psyr_1714 at 1942.673 kb on + strand, within Psyr_1714 at 1942.703 kb on - strand, within Psyr_1714 at 1942.932 kb on - strand at 1943.102 kb on + strand at 1943.103 kb on - strand at 1943.103 kb on - strand at 1943.865 kb on + strand, within Psyr_1715 at 1943.865 kb on + strand, within Psyr_1715 at 1943.865 kb on + strand, within Psyr_1715 at 1943.865 kb on + strand, within Psyr_1715 at 1943.865 kb on + strand, within Psyr_1715 at 1943.865 kb on + strand, within Psyr_1715 at 1943.865 kb on + strand, within Psyr_1715 at 1943.866 kb on - strand, within Psyr_1715 at 1943.866 kb on - strand, within Psyr_1715 at 1943.866 kb on - strand, within Psyr_1715 at 1943.866 kb on - strand, within Psyr_1715 at 1943.866 kb on - strand, within Psyr_1715 at 1943.866 kb on - strand, within Psyr_1715 at 1943.866 kb on - strand, within Psyr_1715 at 1944.061 kb on - strand, within Psyr_1715 at 1944.343 kb on - strand, within Psyr_1715 at 1944.407 kb on + strand, within Psyr_1715 at 1944.407 kb on + strand, within Psyr_1715 at 1944.407 kb on + strand, within Psyr_1715 at 1944.407 kb on + strand, within Psyr_1715 at 1944.407 kb on + strand, within Psyr_1715 at 1944.407 kb on + strand, within Psyr_1715 at 1944.407 kb on + strand, within Psyr_1715 at 1944.407 kb on + strand, within Psyr_1715 at 1944.407 kb on + strand, within Psyr_1715 at 1944.407 kb on + strand, within Psyr_1715 at 1944.408 kb on - strand at 1944.408 kb on - strand at 1944.408 kb on - strand at 1944.408 kb on - strand at 1944.408 kb on - strand at 1944.408 kb on - strand at 1944.408 kb on - strand at 1944.408 kb on - strand at 1944.408 kb on - strand at 1944.539 kb on - strand at 1944.549 kb on - strand at 1944.550 kb on + strand at 1944.577 kb on - strand at 1944.577 kb on - strand at 1944.625 kb on + strand at 1944.625 kb on + strand at 1944.625 kb on + strand at 1944.625 kb on + strand at 1944.625 kb on + strand at 1944.626 kb on - strand at 1944.626 kb on - strand at 1944.699 kb on + strand at 1944.700 kb on - strand at 1945.360 kb on + strand, within Psyr_1716 at 1945.399 kb on + strand, within Psyr_1716
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Brucine 10 mM remove 1,942,673 + Psyr_1714 0.83 -0.6 1,942,673 + Psyr_1714 0.83 +1.2 1,942,673 + Psyr_1714 0.83 -0.9 1,942,703 - Psyr_1714 0.84 -0.5 1,942,932 - +2.2 1,943,102 + +0.2 1,943,103 - -0.7 1,943,103 - +0.3 1,943,865 + Psyr_1715 0.46 +0.3 1,943,865 + Psyr_1715 0.46 +1.2 1,943,865 + Psyr_1715 0.46 +1.0 1,943,865 + Psyr_1715 0.46 +0.5 1,943,865 + Psyr_1715 0.46 +0.5 1,943,865 + Psyr_1715 0.46 +0.3 1,943,865 + Psyr_1715 0.46 +0.7 1,943,866 - Psyr_1715 0.46 -0.8 1,943,866 - Psyr_1715 0.46 +0.2 1,943,866 - Psyr_1715 0.46 -1.1 1,943,866 - Psyr_1715 0.46 +0.1 1,943,866 - Psyr_1715 0.46 +1.4 1,943,866 - Psyr_1715 0.46 -0.5 1,943,866 - Psyr_1715 0.46 +1.5 1,944,061 - Psyr_1715 0.62 -1.8 1,944,343 - Psyr_1715 0.85 -2.0 1,944,407 + Psyr_1715 0.90 -1.1 1,944,407 + Psyr_1715 0.90 +0.4 1,944,407 + Psyr_1715 0.90 +1.9 1,944,407 + Psyr_1715 0.90 +1.3 1,944,407 + Psyr_1715 0.90 -0.7 1,944,407 + Psyr_1715 0.90 +1.2 1,944,407 + Psyr_1715 0.90 +0.9 1,944,407 + Psyr_1715 0.90 -0.3 1,944,407 + Psyr_1715 0.90 +0.6 1,944,407 + Psyr_1715 0.90 +1.6 1,944,408 - +0.3 1,944,408 - -0.5 1,944,408 - -0.3 1,944,408 - -1.6 1,944,408 - +2.8 1,944,408 - -0.5 1,944,408 - -2.4 1,944,408 - -0.2 1,944,408 - -0.6 1,944,539 - +0.3 1,944,549 - +0.6 1,944,550 + -1.0 1,944,577 - +0.3 1,944,577 - +0.3 1,944,625 + +0.2 1,944,625 + -0.2 1,944,625 + -0.3 1,944,625 + -0.1 1,944,625 + -1.6 1,944,626 - +0.0 1,944,626 - -0.8 1,944,699 + +1.3 1,944,700 - +0.0 1,945,360 + Psyr_1716 0.47 -1.3 1,945,399 + Psyr_1716 0.50 -0.8
Or see this region's nucleotide sequence