Experiment: KB with Brucine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1447 and Psyr_1448 are separated by 15 nucleotides Psyr_1448 and Psyr_1449 are separated by 19 nucleotides Psyr_1449 and Psyr_1450 overlap by 4 nucleotides Psyr_1450 and Psyr_1451 are separated by 79 nucleotides Psyr_1451 and Psyr_1452 are separated by 9 nucleotides
Psyr_1447: Psyr_1447 - conserved hypothetical protein, at 1,636,875 to 1,637,267
_1447
Psyr_1448: Psyr_1448 - conserved hypothetical protein, at 1,637,283 to 1,637,651
_1448
Psyr_1449: Psyr_1449 - hypothetical protein, at 1,637,671 to 1,637,910
_1449
Psyr_1450: Psyr_1450 - Plasmid conserved hypothetical protein, RAQPRD, at 1,637,907 to 1,638,269
_1450
Psyr_1451: Psyr_1451 - hypothetical protein, at 1,638,349 to 1,638,681
_1451
Psyr_1452: Psyr_1452 - conserved hypothetical protein, at 1,638,691 to 1,640,154
_1452
Position (kb) 1637
1638
1639 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1636.968 kb on + strand, within Psyr_1447 at 1636.969 kb on - strand, within Psyr_1447 at 1637.062 kb on - strand, within Psyr_1447 at 1637.175 kb on - strand, within Psyr_1447 at 1637.277 kb on + strand at 1637.314 kb on + strand at 1637.314 kb on + strand at 1637.315 kb on - strand at 1637.315 kb on - strand at 1637.315 kb on - strand at 1637.450 kb on + strand, within Psyr_1448 at 1637.450 kb on + strand, within Psyr_1448 at 1637.450 kb on + strand, within Psyr_1448 at 1637.450 kb on + strand, within Psyr_1448 at 1637.450 kb on + strand, within Psyr_1448 at 1637.450 kb on + strand, within Psyr_1448 at 1637.450 kb on + strand, within Psyr_1448 at 1637.450 kb on + strand, within Psyr_1448 at 1637.450 kb on + strand, within Psyr_1448 at 1637.450 kb on + strand, within Psyr_1448 at 1637.451 kb on - strand, within Psyr_1448 at 1637.451 kb on - strand, within Psyr_1448 at 1637.451 kb on - strand, within Psyr_1448 at 1637.451 kb on - strand, within Psyr_1448 at 1637.485 kb on + strand, within Psyr_1448 at 1637.485 kb on + strand, within Psyr_1448 at 1637.485 kb on + strand, within Psyr_1448 at 1637.510 kb on + strand, within Psyr_1448 at 1637.510 kb on + strand, within Psyr_1448 at 1637.511 kb on - strand, within Psyr_1448 at 1637.561 kb on + strand, within Psyr_1448 at 1637.561 kb on + strand, within Psyr_1448 at 1637.561 kb on + strand, within Psyr_1448 at 1637.655 kb on + strand at 1637.655 kb on + strand at 1637.694 kb on + strand at 1637.694 kb on + strand at 1637.745 kb on + strand, within Psyr_1449 at 1637.745 kb on + strand, within Psyr_1449 at 1637.775 kb on - strand, within Psyr_1449 at 1637.775 kb on - strand, within Psyr_1449 at 1637.782 kb on + strand, within Psyr_1449 at 1637.782 kb on + strand, within Psyr_1449 at 1637.782 kb on + strand, within Psyr_1449 at 1637.803 kb on + strand, within Psyr_1449 at 1637.936 kb on - strand at 1637.936 kb on - strand at 1638.175 kb on + strand, within Psyr_1450 at 1638.175 kb on + strand, within Psyr_1450 at 1638.175 kb on + strand, within Psyr_1450 at 1638.175 kb on + strand, within Psyr_1450 at 1638.176 kb on - strand, within Psyr_1450 at 1638.176 kb on - strand, within Psyr_1450 at 1638.176 kb on - strand, within Psyr_1450 at 1638.176 kb on - strand, within Psyr_1450 at 1638.198 kb on + strand, within Psyr_1450 at 1638.198 kb on + strand, within Psyr_1450 at 1638.198 kb on + strand, within Psyr_1450 at 1638.199 kb on - strand, within Psyr_1450 at 1638.199 kb on - strand, within Psyr_1450 at 1638.199 kb on - strand, within Psyr_1450 at 1638.290 kb on + strand at 1638.291 kb on - strand at 1638.298 kb on + strand at 1638.298 kb on + strand at 1638.298 kb on + strand at 1638.298 kb on + strand at 1638.298 kb on + strand at 1638.298 kb on + strand at 1638.298 kb on + strand at 1638.299 kb on - strand at 1638.299 kb on - strand at 1638.299 kb on - strand at 1638.299 kb on - strand at 1638.299 kb on - strand at 1638.336 kb on + strand at 1638.351 kb on - strand at 1638.351 kb on - strand at 1638.351 kb on - strand at 1638.588 kb on + strand, within Psyr_1451 at 1638.666 kb on - strand at 1638.793 kb on + strand at 1638.793 kb on + strand at 1638.793 kb on + strand at 1638.793 kb on + strand at 1638.793 kb on + strand at 1638.793 kb on + strand at 1638.793 kb on + strand at 1638.793 kb on + strand at 1638.793 kb on + strand at 1638.793 kb on + strand at 1638.793 kb on + strand at 1638.793 kb on + strand at 1638.793 kb on + strand at 1638.794 kb on - strand at 1638.794 kb on - strand at 1638.794 kb on - strand at 1638.794 kb on - strand at 1638.794 kb on - strand at 1638.794 kb on - strand at 1638.794 kb on - strand at 1638.794 kb on - strand at 1638.794 kb on - strand at 1638.794 kb on - strand at 1638.794 kb on - strand at 1638.797 kb on + strand at 1638.797 kb on + strand at 1638.797 kb on + strand at 1638.797 kb on + strand at 1638.797 kb on + strand at 1638.797 kb on + strand at 1638.797 kb on + strand at 1638.797 kb on + strand at 1638.797 kb on + strand at 1638.797 kb on + strand at 1638.797 kb on + strand at 1638.797 kb on + strand at 1638.797 kb on + strand at 1638.797 kb on + strand at 1638.797 kb on + strand at 1638.797 kb on + strand at 1638.798 kb on - strand at 1638.798 kb on - strand at 1638.798 kb on - strand at 1638.798 kb on - strand at 1638.798 kb on - strand at 1638.798 kb on - strand at 1638.798 kb on - strand at 1638.798 kb on - strand at 1638.798 kb on - strand at 1638.799 kb on + strand at 1638.799 kb on + strand at 1638.799 kb on + strand at 1638.799 kb on + strand at 1638.799 kb on + strand at 1638.799 kb on + strand at 1638.799 kb on + strand at 1638.799 kb on + strand at 1638.799 kb on + strand at 1638.800 kb on - strand at 1638.800 kb on - strand at 1638.800 kb on - strand at 1638.800 kb on - strand at 1638.800 kb on - strand at 1638.800 kb on - strand at 1638.800 kb on - strand at 1638.800 kb on - strand at 1638.800 kb on - strand at 1638.872 kb on + strand, within Psyr_1452 at 1638.872 kb on + strand, within Psyr_1452 at 1638.872 kb on + strand, within Psyr_1452 at 1638.921 kb on + strand, within Psyr_1452 at 1638.998 kb on + strand, within Psyr_1452 at 1638.999 kb on - strand, within Psyr_1452 at 1639.064 kb on + strand, within Psyr_1452 at 1639.064 kb on + strand, within Psyr_1452 at 1639.064 kb on + strand, within Psyr_1452 at 1639.064 kb on + strand, within Psyr_1452 at 1639.064 kb on + strand, within Psyr_1452 at 1639.064 kb on + strand, within Psyr_1452 at 1639.065 kb on - strand, within Psyr_1452 at 1639.065 kb on - strand, within Psyr_1452 at 1639.065 kb on - strand, within Psyr_1452 at 1639.065 kb on - strand, within Psyr_1452 at 1639.083 kb on - strand, within Psyr_1452 at 1639.122 kb on + strand, within Psyr_1452 at 1639.122 kb on + strand, within Psyr_1452 at 1639.122 kb on + strand, within Psyr_1452 at 1639.123 kb on - strand, within Psyr_1452 at 1639.136 kb on + strand, within Psyr_1452 at 1639.136 kb on + strand, within Psyr_1452 at 1639.137 kb on - strand at 1639.166 kb on + strand, within Psyr_1452 at 1639.166 kb on + strand, within Psyr_1452 at 1639.166 kb on + strand, within Psyr_1452 at 1639.166 kb on + strand, within Psyr_1452 at 1639.166 kb on + strand, within Psyr_1452 at 1639.167 kb on - strand, within Psyr_1452 at 1639.167 kb on - strand, within Psyr_1452 at 1639.167 kb on - strand, within Psyr_1452 at 1639.167 kb on - strand, within Psyr_1452 at 1639.167 kb on - strand, within Psyr_1452 at 1639.167 kb on - strand, within Psyr_1452 at 1639.248 kb on + strand, within Psyr_1452 at 1639.248 kb on + strand, within Psyr_1452 at 1639.249 kb on - strand, within Psyr_1452 at 1639.259 kb on + strand, within Psyr_1452 at 1639.260 kb on - strand, within Psyr_1452 at 1639.260 kb on - strand, within Psyr_1452
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Brucine 10 mM remove 1,636,968 + Psyr_1447 0.24 -0.3 1,636,969 - Psyr_1447 0.24 -0.3 1,637,062 - Psyr_1447 0.48 +0.8 1,637,175 - Psyr_1447 0.76 +1.1 1,637,277 + +0.1 1,637,314 + -0.0 1,637,314 + +0.4 1,637,315 - -2.7 1,637,315 - +1.3 1,637,315 - -1.1 1,637,450 + Psyr_1448 0.45 +0.7 1,637,450 + Psyr_1448 0.45 +1.0 1,637,450 + Psyr_1448 0.45 +1.4 1,637,450 + Psyr_1448 0.45 +1.4 1,637,450 + Psyr_1448 0.45 +0.4 1,637,450 + Psyr_1448 0.45 +0.1 1,637,450 + Psyr_1448 0.45 -0.5 1,637,450 + Psyr_1448 0.45 -0.2 1,637,450 + Psyr_1448 0.45 +2.7 1,637,450 + Psyr_1448 0.45 -0.5 1,637,451 - Psyr_1448 0.46 +1.0 1,637,451 - Psyr_1448 0.46 +1.8 1,637,451 - Psyr_1448 0.46 +0.3 1,637,451 - Psyr_1448 0.46 +0.8 1,637,485 + Psyr_1448 0.55 +1.6 1,637,485 + Psyr_1448 0.55 -0.1 1,637,485 + Psyr_1448 0.55 +0.7 1,637,510 + Psyr_1448 0.62 -0.9 1,637,510 + Psyr_1448 0.62 +0.7 1,637,511 - Psyr_1448 0.62 +0.5 1,637,561 + Psyr_1448 0.75 +0.1 1,637,561 + Psyr_1448 0.75 -0.3 1,637,561 + Psyr_1448 0.75 -1.3 1,637,655 + -0.0 1,637,655 + -0.9 1,637,694 + -0.2 1,637,694 + -1.2 1,637,745 + Psyr_1449 0.31 -0.7 1,637,745 + Psyr_1449 0.31 -1.0 1,637,775 - Psyr_1449 0.43 -0.4 1,637,775 - Psyr_1449 0.43 -1.0 1,637,782 + Psyr_1449 0.46 +0.5 1,637,782 + Psyr_1449 0.46 -0.5 1,637,782 + Psyr_1449 0.46 +0.1 1,637,803 + Psyr_1449 0.55 -0.9 1,637,936 - +0.1 1,637,936 - +0.3 1,638,175 + Psyr_1450 0.74 +2.0 1,638,175 + Psyr_1450 0.74 +0.4 1,638,175 + Psyr_1450 0.74 -0.6 1,638,175 + Psyr_1450 0.74 +0.3 1,638,176 - Psyr_1450 0.74 -0.6 1,638,176 - Psyr_1450 0.74 -0.2 1,638,176 - Psyr_1450 0.74 -0.0 1,638,176 - Psyr_1450 0.74 +0.1 1,638,198 + Psyr_1450 0.80 +0.6 1,638,198 + Psyr_1450 0.80 -0.9 1,638,198 + Psyr_1450 0.80 -0.3 1,638,199 - Psyr_1450 0.80 -0.0 1,638,199 - Psyr_1450 0.80 -1.0 1,638,199 - Psyr_1450 0.80 +0.1 1,638,290 + +1.0 1,638,291 - +0.3 1,638,298 + -0.1 1,638,298 + +0.1 1,638,298 + +0.8 1,638,298 + +0.5 1,638,298 + +1.1 1,638,298 + +0.2 1,638,298 + -0.5 1,638,299 - -1.3 1,638,299 - -0.3 1,638,299 - -1.2 1,638,299 - +0.3 1,638,299 - +0.5 1,638,336 + +0.3 1,638,351 - -0.0 1,638,351 - -1.4 1,638,351 - +0.3 1,638,588 + Psyr_1451 0.72 +0.5 1,638,666 - +0.4 1,638,793 + +0.2 1,638,793 + +0.5 1,638,793 + +0.1 1,638,793 + -0.5 1,638,793 + +0.1 1,638,793 + +3.6 1,638,793 + +0.3 1,638,793 + -0.5 1,638,793 + -1.9 1,638,793 + +0.2 1,638,793 + +1.0 1,638,793 + +0.1 1,638,793 + -0.7 1,638,794 - +0.6 1,638,794 - -0.3 1,638,794 - -1.3 1,638,794 - -1.3 1,638,794 - +0.4 1,638,794 - -1.7 1,638,794 - +1.6 1,638,794 - -0.5 1,638,794 - -1.2 1,638,794 - -0.0 1,638,794 - +0.1 1,638,797 + +0.4 1,638,797 + +0.7 1,638,797 + -0.2 1,638,797 + -1.9 1,638,797 + -1.7 1,638,797 + -0.5 1,638,797 + +0.3 1,638,797 + +0.5 1,638,797 + +0.8 1,638,797 + +0.6 1,638,797 + +0.4 1,638,797 + -1.0 1,638,797 + -1.9 1,638,797 + -0.5 1,638,797 + -0.4 1,638,797 + -0.0 1,638,798 - -2.4 1,638,798 - -1.2 1,638,798 - -1.1 1,638,798 - -0.5 1,638,798 - -0.2 1,638,798 - +1.3 1,638,798 - -1.3 1,638,798 - +0.2 1,638,798 - -1.5 1,638,799 + +0.7 1,638,799 + -0.0 1,638,799 + -0.7 1,638,799 + +0.3 1,638,799 + -0.8 1,638,799 + +0.3 1,638,799 + -0.3 1,638,799 + -0.4 1,638,799 + -0.7 1,638,800 - -0.7 1,638,800 - -0.4 1,638,800 - -2.3 1,638,800 - +0.0 1,638,800 - +0.2 1,638,800 - +0.1 1,638,800 - -0.0 1,638,800 - -0.9 1,638,800 - -0.2 1,638,872 + Psyr_1452 0.12 +1.0 1,638,872 + Psyr_1452 0.12 -0.1 1,638,872 + Psyr_1452 0.12 +0.3 1,638,921 + Psyr_1452 0.16 +0.5 1,638,998 + Psyr_1452 0.21 +0.8 1,638,999 - Psyr_1452 0.21 +0.3 1,639,064 + Psyr_1452 0.25 +0.4 1,639,064 + Psyr_1452 0.25 -0.3 1,639,064 + Psyr_1452 0.25 -0.0 1,639,064 + Psyr_1452 0.25 +0.3 1,639,064 + Psyr_1452 0.25 -0.9 1,639,064 + Psyr_1452 0.25 +0.7 1,639,065 - Psyr_1452 0.26 +0.6 1,639,065 - Psyr_1452 0.26 -1.3 1,639,065 - Psyr_1452 0.26 +0.7 1,639,065 - Psyr_1452 0.26 +0.3 1,639,083 - Psyr_1452 0.27 +0.4 1,639,122 + Psyr_1452 0.29 -1.8 1,639,122 + Psyr_1452 0.29 +0.4 1,639,122 + Psyr_1452 0.29 -0.1 1,639,123 - Psyr_1452 0.30 +0.0 1,639,136 + Psyr_1452 0.30 +2.9 1,639,136 + Psyr_1452 0.30 -0.2 1,639,137 - +0.3 1,639,166 + Psyr_1452 0.32 +0.8 1,639,166 + Psyr_1452 0.32 -2.2 1,639,166 + Psyr_1452 0.32 +1.5 1,639,166 + Psyr_1452 0.32 +0.5 1,639,166 + Psyr_1452 0.32 -0.4 1,639,167 - Psyr_1452 0.33 +0.2 1,639,167 - Psyr_1452 0.33 +0.8 1,639,167 - Psyr_1452 0.33 +0.1 1,639,167 - Psyr_1452 0.33 +0.3 1,639,167 - Psyr_1452 0.33 +0.1 1,639,167 - Psyr_1452 0.33 -0.1 1,639,248 + Psyr_1452 0.38 -0.7 1,639,248 + Psyr_1452 0.38 -0.9 1,639,249 - Psyr_1452 0.38 -1.0 1,639,259 + Psyr_1452 0.39 +0.4 1,639,260 - Psyr_1452 0.39 +0.1 1,639,260 - Psyr_1452 0.39 -0.5
Or see this region's nucleotide sequence