Experiment: KB with Brucine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0863 and Psyr_0864 are separated by 108 nucleotides Psyr_0864 and Psyr_0865 are separated by 267 nucleotides Psyr_0865 and Psyr_0866 are separated by 65 nucleotides Psyr_0866 and Psyr_0867 are separated by 86 nucleotides
Psyr_0863: Psyr_0863 - PhnA protein, at 983,673 to 984,047
_0863
Psyr_0864: Psyr_0864 - Protein of unknown function DUF393, at 984,156 to 984,587
_0864
Psyr_0865: Psyr_0865 - General substrate transporter:Major facilitator superfamily, at 984,855 to 986,063
_0865
Psyr_0866: Psyr_0866 - CDP-diacylglycerol-choline O-phosphatidyltransferase, at 986,129 to 986,848
_0866
Psyr_0867: Psyr_0867 - conserved hypothetical protein, at 986,935 to 988,947
_0867
Position (kb)
984
985
986
987 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 984.353 kb on + strand, within Psyr_0864 at 984.354 kb on - strand, within Psyr_0864 at 984.354 kb on - strand, within Psyr_0864 at 984.489 kb on + strand, within Psyr_0864 at 984.490 kb on - strand, within Psyr_0864 at 984.531 kb on + strand, within Psyr_0864 at 984.531 kb on + strand, within Psyr_0864 at 984.605 kb on + strand at 984.605 kb on + strand at 984.606 kb on - strand at 984.652 kb on + strand at 984.692 kb on + strand at 984.693 kb on - strand at 984.781 kb on + strand at 984.781 kb on + strand at 984.782 kb on - strand at 984.782 kb on - strand at 984.860 kb on - strand at 984.887 kb on + strand at 984.888 kb on - strand at 984.888 kb on - strand at 984.968 kb on + strand at 984.968 kb on + strand at 984.968 kb on + strand at 984.969 kb on - strand at 985.026 kb on + strand, within Psyr_0865 at 985.026 kb on + strand, within Psyr_0865 at 985.026 kb on + strand, within Psyr_0865 at 985.026 kb on + strand, within Psyr_0865 at 985.026 kb on + strand, within Psyr_0865 at 985.026 kb on + strand, within Psyr_0865 at 985.026 kb on + strand, within Psyr_0865 at 985.026 kb on + strand, within Psyr_0865 at 985.026 kb on + strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.027 kb on - strand, within Psyr_0865 at 985.292 kb on + strand, within Psyr_0865 at 985.310 kb on - strand, within Psyr_0865 at 985.310 kb on - strand, within Psyr_0865 at 985.396 kb on + strand, within Psyr_0865 at 985.396 kb on + strand, within Psyr_0865 at 985.397 kb on - strand, within Psyr_0865 at 985.500 kb on + strand, within Psyr_0865 at 985.500 kb on + strand, within Psyr_0865 at 985.501 kb on - strand, within Psyr_0865 at 985.501 kb on - strand, within Psyr_0865 at 985.501 kb on - strand, within Psyr_0865 at 985.542 kb on + strand, within Psyr_0865 at 985.543 kb on - strand, within Psyr_0865 at 985.548 kb on + strand, within Psyr_0865 at 985.548 kb on + strand, within Psyr_0865 at 985.548 kb on + strand, within Psyr_0865 at 985.548 kb on + strand, within Psyr_0865 at 985.548 kb on + strand, within Psyr_0865 at 985.548 kb on + strand, within Psyr_0865 at 985.548 kb on + strand, within Psyr_0865 at 985.548 kb on + strand, within Psyr_0865 at 985.548 kb on + strand, within Psyr_0865 at 985.548 kb on + strand, within Psyr_0865 at 985.548 kb on + strand, within Psyr_0865 at 985.548 kb on + strand, within Psyr_0865 at 985.548 kb on + strand, within Psyr_0865 at 985.548 kb on + strand, within Psyr_0865 at 985.548 kb on + strand, within Psyr_0865 at 985.549 kb on - strand, within Psyr_0865 at 985.549 kb on - strand, within Psyr_0865 at 985.549 kb on - strand, within Psyr_0865 at 985.549 kb on - strand, within Psyr_0865 at 985.549 kb on - strand, within Psyr_0865 at 985.549 kb on - strand, within Psyr_0865 at 985.549 kb on - strand, within Psyr_0865 at 985.549 kb on - strand, within Psyr_0865 at 985.549 kb on - strand, within Psyr_0865 at 985.549 kb on - strand, within Psyr_0865 at 985.775 kb on + strand, within Psyr_0865 at 985.776 kb on - strand, within Psyr_0865 at 986.001 kb on + strand at 986.061 kb on + strand at 986.061 kb on + strand at 986.062 kb on - strand at 986.138 kb on - strand at 986.174 kb on - strand at 986.174 kb on - strand at 986.190 kb on + strand at 986.190 kb on + strand at 986.276 kb on + strand, within Psyr_0866 at 986.276 kb on + strand, within Psyr_0866 at 986.276 kb on + strand, within Psyr_0866 at 986.277 kb on - strand, within Psyr_0866 at 986.277 kb on - strand, within Psyr_0866 at 986.412 kb on + strand, within Psyr_0866 at 986.412 kb on + strand, within Psyr_0866 at 986.412 kb on + strand, within Psyr_0866 at 986.412 kb on + strand, within Psyr_0866 at 986.413 kb on - strand, within Psyr_0866 at 986.413 kb on - strand, within Psyr_0866 at 986.413 kb on - strand, within Psyr_0866 at 986.460 kb on + strand, within Psyr_0866 at 986.460 kb on + strand, within Psyr_0866 at 986.460 kb on + strand, within Psyr_0866 at 986.460 kb on + strand, within Psyr_0866 at 986.460 kb on + strand, within Psyr_0866 at 986.460 kb on + strand, within Psyr_0866 at 986.460 kb on + strand, within Psyr_0866 at 986.461 kb on - strand, within Psyr_0866 at 986.461 kb on - strand, within Psyr_0866 at 986.461 kb on - strand, within Psyr_0866 at 986.461 kb on - strand, within Psyr_0866 at 986.558 kb on + strand, within Psyr_0866 at 986.559 kb on - strand, within Psyr_0866 at 986.559 kb on - strand, within Psyr_0866 at 986.560 kb on + strand, within Psyr_0866 at 986.560 kb on + strand, within Psyr_0866 at 986.560 kb on + strand, within Psyr_0866 at 986.560 kb on + strand, within Psyr_0866 at 986.560 kb on + strand, within Psyr_0866 at 986.560 kb on + strand, within Psyr_0866 at 986.560 kb on + strand, within Psyr_0866 at 986.560 kb on + strand, within Psyr_0866 at 986.560 kb on + strand, within Psyr_0866 at 986.561 kb on - strand, within Psyr_0866 at 986.561 kb on - strand, within Psyr_0866 at 986.561 kb on - strand, within Psyr_0866 at 986.561 kb on - strand, within Psyr_0866 at 986.561 kb on - strand, within Psyr_0866 at 986.561 kb on - strand, within Psyr_0866 at 986.562 kb on + strand, within Psyr_0866 at 986.562 kb on + strand, within Psyr_0866 at 986.562 kb on + strand, within Psyr_0866 at 986.562 kb on + strand, within Psyr_0866 at 986.562 kb on + strand, within Psyr_0866 at 986.562 kb on + strand, within Psyr_0866 at 986.563 kb on - strand, within Psyr_0866 at 986.563 kb on - strand, within Psyr_0866 at 986.568 kb on + strand, within Psyr_0866 at 986.568 kb on + strand, within Psyr_0866 at 986.568 kb on + strand, within Psyr_0866 at 986.568 kb on + strand, within Psyr_0866 at 986.569 kb on - strand, within Psyr_0866 at 986.595 kb on + strand, within Psyr_0866 at 986.596 kb on - strand, within Psyr_0866 at 986.596 kb on - strand, within Psyr_0866 at 986.596 kb on - strand, within Psyr_0866 at 986.596 kb on - strand, within Psyr_0866 at 986.596 kb on - strand, within Psyr_0866 at 986.596 kb on - strand, within Psyr_0866 at 986.781 kb on - strand at 986.868 kb on + strand at 986.868 kb on + strand at 986.868 kb on + strand at 986.869 kb on - strand at 986.869 kb on - strand at 986.869 kb on - strand at 986.869 kb on - strand at 986.909 kb on + strand at 986.931 kb on - strand at 987.008 kb on + strand at 987.008 kb on + strand at 987.008 kb on + strand at 987.008 kb on + strand at 987.009 kb on - strand at 987.009 kb on - strand at 987.033 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Brucine 10 mM remove 984,353 + Psyr_0864 0.46 -1.0 984,354 - Psyr_0864 0.46 +0.4 984,354 - Psyr_0864 0.46 -0.7 984,489 + Psyr_0864 0.77 -0.1 984,490 - Psyr_0864 0.77 -0.1 984,531 + Psyr_0864 0.87 -2.4 984,531 + Psyr_0864 0.87 -2.8 984,605 + -0.2 984,605 + -0.8 984,606 - +0.2 984,652 + +0.4 984,692 + +0.0 984,693 - +0.7 984,781 + +0.7 984,781 + +0.0 984,782 - +1.2 984,782 - +0.2 984,860 - +2.2 984,887 + -0.9 984,888 - -0.3 984,888 - -0.9 984,968 + +0.4 984,968 + -0.1 984,968 + +0.8 984,969 - -0.7 985,026 + Psyr_0865 0.14 +0.7 985,026 + Psyr_0865 0.14 +0.1 985,026 + Psyr_0865 0.14 +1.0 985,026 + Psyr_0865 0.14 -0.6 985,026 + Psyr_0865 0.14 +0.5 985,026 + Psyr_0865 0.14 -0.4 985,026 + Psyr_0865 0.14 -0.9 985,026 + Psyr_0865 0.14 +0.7 985,026 + Psyr_0865 0.14 +0.0 985,027 - Psyr_0865 0.14 +0.6 985,027 - Psyr_0865 0.14 +0.4 985,027 - Psyr_0865 0.14 -1.6 985,027 - Psyr_0865 0.14 -1.2 985,027 - Psyr_0865 0.14 -1.4 985,027 - Psyr_0865 0.14 +0.1 985,027 - Psyr_0865 0.14 +0.6 985,027 - Psyr_0865 0.14 +0.7 985,027 - Psyr_0865 0.14 +0.7 985,027 - Psyr_0865 0.14 -1.6 985,027 - Psyr_0865 0.14 -0.6 985,027 - Psyr_0865 0.14 +0.1 985,027 - Psyr_0865 0.14 -0.4 985,027 - Psyr_0865 0.14 -0.3 985,027 - Psyr_0865 0.14 -1.0 985,027 - Psyr_0865 0.14 +0.2 985,027 - Psyr_0865 0.14 -0.1 985,292 + Psyr_0865 0.36 -0.6 985,310 - Psyr_0865 0.38 +0.2 985,310 - Psyr_0865 0.38 -0.0 985,396 + Psyr_0865 0.45 +0.1 985,396 + Psyr_0865 0.45 -0.8 985,397 - Psyr_0865 0.45 +0.6 985,500 + Psyr_0865 0.53 -0.2 985,500 + Psyr_0865 0.53 +1.0 985,501 - Psyr_0865 0.53 +0.7 985,501 - Psyr_0865 0.53 -0.1 985,501 - Psyr_0865 0.53 +0.4 985,542 + Psyr_0865 0.57 +0.0 985,543 - Psyr_0865 0.57 -0.1 985,548 + Psyr_0865 0.57 +0.3 985,548 + Psyr_0865 0.57 -0.8 985,548 + Psyr_0865 0.57 +0.0 985,548 + Psyr_0865 0.57 -0.4 985,548 + Psyr_0865 0.57 +0.9 985,548 + Psyr_0865 0.57 +0.2 985,548 + Psyr_0865 0.57 +0.8 985,548 + Psyr_0865 0.57 +0.1 985,548 + Psyr_0865 0.57 +0.1 985,548 + Psyr_0865 0.57 +1.2 985,548 + Psyr_0865 0.57 -0.8 985,548 + Psyr_0865 0.57 +1.4 985,548 + Psyr_0865 0.57 -0.2 985,548 + Psyr_0865 0.57 -0.6 985,548 + Psyr_0865 0.57 -2.0 985,549 - Psyr_0865 0.57 -1.7 985,549 - Psyr_0865 0.57 -0.3 985,549 - Psyr_0865 0.57 +2.2 985,549 - Psyr_0865 0.57 -1.0 985,549 - Psyr_0865 0.57 +0.2 985,549 - Psyr_0865 0.57 +0.1 985,549 - Psyr_0865 0.57 -0.7 985,549 - Psyr_0865 0.57 +2.1 985,549 - Psyr_0865 0.57 -0.2 985,549 - Psyr_0865 0.57 -1.4 985,775 + Psyr_0865 0.76 -0.4 985,776 - Psyr_0865 0.76 +0.4 986,001 + -0.1 986,061 + +0.5 986,061 + -1.0 986,062 - +1.2 986,138 - +1.3 986,174 - +0.4 986,174 - -1.0 986,190 + -0.4 986,190 + +0.3 986,276 + Psyr_0866 0.20 -0.5 986,276 + Psyr_0866 0.20 -1.5 986,276 + Psyr_0866 0.20 -0.5 986,277 - Psyr_0866 0.21 -1.4 986,277 - Psyr_0866 0.21 +1.4 986,412 + Psyr_0866 0.39 +0.3 986,412 + Psyr_0866 0.39 -1.5 986,412 + Psyr_0866 0.39 -0.3 986,412 + Psyr_0866 0.39 -0.7 986,413 - Psyr_0866 0.39 +0.0 986,413 - Psyr_0866 0.39 -0.2 986,413 - Psyr_0866 0.39 -0.0 986,460 + Psyr_0866 0.46 +0.0 986,460 + Psyr_0866 0.46 +0.2 986,460 + Psyr_0866 0.46 -2.4 986,460 + Psyr_0866 0.46 -2.0 986,460 + Psyr_0866 0.46 -0.3 986,460 + Psyr_0866 0.46 -1.6 986,460 + Psyr_0866 0.46 -0.9 986,461 - Psyr_0866 0.46 -0.4 986,461 - Psyr_0866 0.46 +0.2 986,461 - Psyr_0866 0.46 +0.6 986,461 - Psyr_0866 0.46 -1.1 986,558 + Psyr_0866 0.60 +0.2 986,559 - Psyr_0866 0.60 +1.7 986,559 - Psyr_0866 0.60 +0.8 986,560 + Psyr_0866 0.60 +2.8 986,560 + Psyr_0866 0.60 -2.5 986,560 + Psyr_0866 0.60 +0.4 986,560 + Psyr_0866 0.60 -0.3 986,560 + Psyr_0866 0.60 -0.6 986,560 + Psyr_0866 0.60 -1.0 986,560 + Psyr_0866 0.60 -0.0 986,560 + Psyr_0866 0.60 -2.8 986,560 + Psyr_0866 0.60 +1.4 986,561 - Psyr_0866 0.60 +0.4 986,561 - Psyr_0866 0.60 -0.4 986,561 - Psyr_0866 0.60 -0.3 986,561 - Psyr_0866 0.60 -0.4 986,561 - Psyr_0866 0.60 +0.6 986,561 - Psyr_0866 0.60 +1.2 986,562 + Psyr_0866 0.60 -1.2 986,562 + Psyr_0866 0.60 -0.7 986,562 + Psyr_0866 0.60 -0.3 986,562 + Psyr_0866 0.60 +0.5 986,562 + Psyr_0866 0.60 -1.5 986,562 + Psyr_0866 0.60 -0.1 986,563 - Psyr_0866 0.60 -0.0 986,563 - Psyr_0866 0.60 +1.2 986,568 + Psyr_0866 0.61 -0.6 986,568 + Psyr_0866 0.61 +0.1 986,568 + Psyr_0866 0.61 +0.2 986,568 + Psyr_0866 0.61 +0.8 986,569 - Psyr_0866 0.61 -0.6 986,595 + Psyr_0866 0.65 -0.6 986,596 - Psyr_0866 0.65 -0.7 986,596 - Psyr_0866 0.65 +1.5 986,596 - Psyr_0866 0.65 -0.5 986,596 - Psyr_0866 0.65 +0.7 986,596 - Psyr_0866 0.65 -0.7 986,596 - Psyr_0866 0.65 -1.5 986,781 - -2.5 986,868 + +0.7 986,868 + +0.3 986,868 + +0.4 986,869 - -1.6 986,869 - +0.2 986,869 - +1.7 986,869 - -0.1 986,909 + -0.5 986,931 - +0.1 987,008 + +0.5 987,008 + +0.4 987,008 + -0.4 987,008 + -0.3 987,009 - +0.9 987,009 - -0.2 987,033 + -0.2
Or see this region's nucleotide sequence