Experiment: KB with Dimethyl Sulfoxide 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3980 and Psyr_3981 are separated by 35 nucleotides Psyr_3981 and Psyr_3982 are separated by 126 nucleotides Psyr_3982 and Psyr_RNA61 are separated by 158 nucleotides Psyr_RNA61 and Psyr_3983 are separated by 361 nucleotides
Psyr_3980: Psyr_3980 - hypothetical protein, at 4,733,916 to 4,734,152
_3980
Psyr_3981: Psyr_3981 - ribosomal small subunit pseudouridine synthase A, at 4,734,188 to 4,734,880
_3981
Psyr_3982: Psyr_3982 - 3-hydroxyacyl-CoA-acyl carrier protein transferase, at 4,735,007 to 4,735,888
_3982
Psyr_RNA61: Psyr_RNA61 - tRNA-Gly, at 4,736,047 to 4,736,120
_RNA61
Psyr_3983: Psyr_3983 - Serralysin, at 4,736,482 to 4,738,839
_3983
Position (kb)
4735
4736 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4734.150 kb on + strand at 4734.150 kb on + strand at 4734.150 kb on + strand at 4734.151 kb on - strand at 4734.151 kb on - strand at 4734.151 kb on - strand at 4734.151 kb on - strand at 4734.151 kb on - strand at 4734.151 kb on - strand at 4734.273 kb on + strand, within Psyr_3981 at 4734.371 kb on + strand, within Psyr_3981 at 4734.372 kb on - strand, within Psyr_3981 at 4734.581 kb on + strand, within Psyr_3981 at 4734.582 kb on - strand, within Psyr_3981 at 4734.582 kb on - strand, within Psyr_3981 at 4734.582 kb on - strand, within Psyr_3981 at 4734.582 kb on - strand, within Psyr_3981 at 4734.584 kb on + strand, within Psyr_3981 at 4734.585 kb on - strand, within Psyr_3981 at 4734.585 kb on - strand, within Psyr_3981 at 4734.645 kb on - strand, within Psyr_3981 at 4734.691 kb on - strand, within Psyr_3981 at 4734.842 kb on + strand at 4734.843 kb on - strand at 4734.980 kb on + strand at 4734.981 kb on - strand at 4734.981 kb on - strand at 4734.981 kb on - strand at 4734.981 kb on - strand at 4734.981 kb on - strand at 4735.006 kb on + strand at 4735.006 kb on + strand at 4735.006 kb on + strand at 4735.006 kb on + strand at 4735.007 kb on - strand at 4735.007 kb on - strand at 4735.007 kb on - strand at 4735.007 kb on - strand at 4735.007 kb on - strand at 4735.007 kb on - strand at 4735.055 kb on + strand at 4735.056 kb on - strand at 4735.181 kb on + strand, within Psyr_3982 at 4735.194 kb on - strand, within Psyr_3982 at 4735.418 kb on + strand, within Psyr_3982 at 4735.419 kb on - strand, within Psyr_3982 at 4735.419 kb on - strand, within Psyr_3982 at 4735.461 kb on - strand, within Psyr_3982 at 4735.508 kb on - strand, within Psyr_3982 at 4735.537 kb on + strand, within Psyr_3982 at 4735.537 kb on + strand, within Psyr_3982 at 4735.537 kb on + strand, within Psyr_3982 at 4735.562 kb on + strand, within Psyr_3982 at 4735.562 kb on + strand, within Psyr_3982 at 4735.578 kb on - strand, within Psyr_3982 at 4735.616 kb on + strand, within Psyr_3982 at 4735.616 kb on + strand, within Psyr_3982 at 4735.616 kb on + strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4736.015 kb on + strand at 4736.055 kb on - strand, within Psyr_RNA61 at 4736.055 kb on - strand, within Psyr_RNA61 at 4736.055 kb on - strand, within Psyr_RNA61 at 4736.055 kb on - strand, within Psyr_RNA61 at 4736.059 kb on + strand, within Psyr_RNA61 at 4736.066 kb on - strand, within Psyr_RNA61 at 4736.088 kb on + strand, within Psyr_RNA61 at 4736.089 kb on - strand, within Psyr_RNA61 at 4736.182 kb on + strand at 4736.182 kb on + strand at 4736.297 kb on - strand at 4736.297 kb on - strand at 4736.304 kb on + strand at 4736.356 kb on + strand at 4736.356 kb on + strand at 4736.357 kb on - strand at 4736.411 kb on + strand at 4736.411 kb on + strand at 4736.664 kb on - strand at 4736.685 kb on + strand at 4736.686 kb on - strand at 4736.686 kb on - strand at 4736.686 kb on - strand at 4736.686 kb on - strand at 4736.830 kb on - strand, within Psyr_3983 at 4736.830 kb on - strand, within Psyr_3983 at 4736.831 kb on + strand, within Psyr_3983 at 4736.831 kb on + strand, within Psyr_3983 at 4736.831 kb on + strand, within Psyr_3983 at 4736.832 kb on - strand, within Psyr_3983 at 4736.832 kb on - strand, within Psyr_3983
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Dimethyl Sulfoxide 1.25 vol% remove 4,734,150 + +0.2 4,734,150 + -1.4 4,734,150 + +1.7 4,734,151 - -0.4 4,734,151 - +0.0 4,734,151 - +0.2 4,734,151 - +0.6 4,734,151 - -0.2 4,734,151 - -0.2 4,734,273 + Psyr_3981 0.12 +0.2 4,734,371 + Psyr_3981 0.26 -0.1 4,734,372 - Psyr_3981 0.27 -1.1 4,734,581 + Psyr_3981 0.57 +0.2 4,734,582 - Psyr_3981 0.57 +0.2 4,734,582 - Psyr_3981 0.57 -0.4 4,734,582 - Psyr_3981 0.57 -0.4 4,734,582 - Psyr_3981 0.57 -0.4 4,734,584 + Psyr_3981 0.57 +0.2 4,734,585 - Psyr_3981 0.57 +0.1 4,734,585 - Psyr_3981 0.57 +1.4 4,734,645 - Psyr_3981 0.66 -0.7 4,734,691 - Psyr_3981 0.73 +0.6 4,734,842 + +0.4 4,734,843 - -0.3 4,734,980 + +1.3 4,734,981 - +0.8 4,734,981 - +0.5 4,734,981 - -0.2 4,734,981 - +1.2 4,734,981 - -0.2 4,735,006 + -0.4 4,735,006 + -1.2 4,735,006 + +1.5 4,735,006 + +0.7 4,735,007 - +1.0 4,735,007 - -0.8 4,735,007 - -0.5 4,735,007 - +2.1 4,735,007 - -0.9 4,735,007 - +2.0 4,735,055 + -0.7 4,735,056 - +1.2 4,735,181 + Psyr_3982 0.20 -0.7 4,735,194 - Psyr_3982 0.21 -0.3 4,735,418 + Psyr_3982 0.47 +0.4 4,735,419 - Psyr_3982 0.47 -0.1 4,735,419 - Psyr_3982 0.47 +1.0 4,735,461 - Psyr_3982 0.51 -0.3 4,735,508 - Psyr_3982 0.57 +0.9 4,735,537 + Psyr_3982 0.60 -0.4 4,735,537 + Psyr_3982 0.60 -0.1 4,735,537 + Psyr_3982 0.60 +2.5 4,735,562 + Psyr_3982 0.63 +0.3 4,735,562 + Psyr_3982 0.63 -0.7 4,735,578 - Psyr_3982 0.65 +0.3 4,735,616 + Psyr_3982 0.69 +0.9 4,735,616 + Psyr_3982 0.69 -0.4 4,735,616 + Psyr_3982 0.69 +0.8 4,735,617 - Psyr_3982 0.69 -0.2 4,735,617 - Psyr_3982 0.69 -0.4 4,735,617 - Psyr_3982 0.69 +0.5 4,735,617 - Psyr_3982 0.69 -0.2 4,735,617 - Psyr_3982 0.69 -0.6 4,735,617 - Psyr_3982 0.69 +0.8 4,735,679 + Psyr_3982 0.76 +0.1 4,735,679 + Psyr_3982 0.76 -0.3 4,735,679 + Psyr_3982 0.76 +0.4 4,735,679 + Psyr_3982 0.76 +0.6 4,735,679 + Psyr_3982 0.76 -0.5 4,735,680 - Psyr_3982 0.76 +0.3 4,735,680 - Psyr_3982 0.76 -1.1 4,735,680 - Psyr_3982 0.76 +0.2 4,735,680 - Psyr_3982 0.76 -0.6 4,735,680 - Psyr_3982 0.76 -0.0 4,735,680 - Psyr_3982 0.76 -0.8 4,736,015 + +1.5 4,736,055 - Psyr_RNA61 0.11 -2.0 4,736,055 - Psyr_RNA61 0.11 -3.0 4,736,055 - Psyr_RNA61 0.11 -0.6 4,736,055 - Psyr_RNA61 0.11 +1.2 4,736,059 + Psyr_RNA61 0.16 -0.9 4,736,066 - Psyr_RNA61 0.26 -0.8 4,736,088 + Psyr_RNA61 0.55 -1.6 4,736,089 - Psyr_RNA61 0.57 -2.3 4,736,182 + -1.2 4,736,182 + -1.2 4,736,297 - +1.0 4,736,297 - +0.3 4,736,304 + +0.2 4,736,356 + -1.4 4,736,356 + -0.4 4,736,357 - -0.7 4,736,411 + -0.4 4,736,411 + +0.1 4,736,664 - +2.2 4,736,685 + +0.4 4,736,686 - -0.8 4,736,686 - +0.4 4,736,686 - -0.8 4,736,686 - +0.5 4,736,830 - Psyr_3983 0.15 +1.0 4,736,830 - Psyr_3983 0.15 +0.2 4,736,831 + Psyr_3983 0.15 -1.4 4,736,831 + Psyr_3983 0.15 -1.0 4,736,831 + Psyr_3983 0.15 +0.6 4,736,832 - Psyr_3983 0.15 -2.4 4,736,832 - Psyr_3983 0.15 +1.1
Or see this region's nucleotide sequence