Experiment: Cisplatin 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1104 and Psest_1105 are separated by 33 nucleotides Psest_1105 and Psest_1106 are separated by 119 nucleotides Psest_1106 and Psest_1107 are separated by 30 nucleotides Psest_1107 and Psest_1108 are separated by 58 nucleotides Psest_1108 and Psest_1109 are separated by 19 nucleotides
Psest_1104: Psest_1104 - peptidyl-tRNA hydrolase, at 1,200,537 to 1,201,121
_1104
Psest_1105: Psest_1105 - ribosomal protein L25, Ctc-form, at 1,201,155 to 1,201,772
_1105
Psest_1106: Psest_1106 - ribose-phosphate pyrophosphokinase, at 1,201,892 to 1,202,881
_1106
Psest_1107: Psest_1107 - tRNA_Lys_TTT, at 1,202,912 to 1,202,987
_1107
Psest_1108: Psest_1108 - tRNA_Gln_TTG, at 1,203,046 to 1,203,120
_1108
Psest_1109: Psest_1109 - 4-diphosphocytidyl-2C-methyl-D-erythritol kinase, at 1,203,140 to 1,204,000
_1109
Position (kb) 1201
1202
1203 Strain fitness (log2 ratio) -1
0
1
2
3 at 1201.863 kb on - strand at 1203.003 kb on - strand at 1203.003 kb on - strand at 1203.003 kb on - strand at 1203.003 kb on - strand at 1203.003 kb on - strand at 1203.028 kb on - strand at 1203.028 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.003 mg/ml remove 1,201,863 - -1.0 1,203,003 - +0.8 1,203,003 - -1.0 1,203,003 - +1.6 1,203,003 - +2.9 1,203,003 - +0.4 1,203,028 - -0.4 1,203,028 - +3.0
Or see this region's nucleotide sequence