Experiment: Cisplatin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2545 and Psest_2546 are separated by 101 nucleotides Psest_2546 and Psest_2547 are separated by 19 nucleotides Psest_2547 and Psest_2548 are separated by 87 nucleotides
Psest_2545: Psest_2545 - recombination protein RecR, at 2,724,555 to 2,725,154
_2545
Psest_2546: Psest_2546 - DNA-binding protein, YbaB/EbfC family, at 2,725,256 to 2,725,582
_2546
Psest_2547: Psest_2547 - DNA polymerase III, subunit gamma and tau, at 2,725,602 to 2,727,614
_2547
Psest_2548: Psest_2548 - Phosphoglycerate dehydrogenase and related dehydrogenases, at 2,727,702 to 2,728,847
_2548
Position (kb)
2725
2726
2727
2728 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2724.694 kb on + strand, within Psest_2545 at 2724.694 kb on + strand, within Psest_2545 at 2724.695 kb on - strand, within Psest_2545 at 2724.789 kb on - strand, within Psest_2545 at 2724.848 kb on - strand, within Psest_2545 at 2724.848 kb on - strand, within Psest_2545 at 2725.096 kb on - strand at 2725.159 kb on - strand at 2725.250 kb on + strand at 2725.250 kb on + strand at 2725.257 kb on + strand at 2725.257 kb on + strand at 2725.258 kb on - strand at 2725.258 kb on - strand at 2725.258 kb on - strand at 2727.692 kb on + strand at 2727.692 kb on + strand at 2727.693 kb on - strand at 2727.693 kb on - strand at 2727.693 kb on - strand at 2727.693 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.0016 mg/ml remove 2,724,694 + Psest_2545 0.23 -0.6 2,724,694 + Psest_2545 0.23 -2.8 2,724,695 - Psest_2545 0.23 -3.1 2,724,789 - Psest_2545 0.39 -2.6 2,724,848 - Psest_2545 0.49 -1.4 2,724,848 - Psest_2545 0.49 -3.9 2,725,096 - -1.9 2,725,159 - -0.8 2,725,250 + -0.9 2,725,250 + -3.8 2,725,257 + -1.6 2,725,257 + -1.6 2,725,258 - -1.4 2,725,258 - +0.5 2,725,258 - +0.9 2,727,692 + -3.1 2,727,692 + -0.2 2,727,693 - +1.4 2,727,693 - +0.6 2,727,693 - -0.6 2,727,693 - +0.3
Or see this region's nucleotide sequence