Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0003

Experiment: Cisplatin 0.0016 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0002 and Psest_0003 are separated by 33 nucleotidesPsest_0003 and Psest_0004 are separated by 13 nucleotides Psest_0002: Psest_0002 - chromosomal replication initiator protein DnaA, at 661 to 2,109 _0002 Psest_0003: Psest_0003 - DNA polymerase III, beta subunit, at 2,143 to 3,246 _0003 Psest_0004: Psest_0004 - recF protein, at 3,260 to 4,372 _0004 Position (kb) 2 3 4Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3.245 kb on - strandat 3.258 kb on + strandat 3.258 kb on + strandat 3.258 kb on + strandat 3.258 kb on + strandat 3.258 kb on + strandat 3.259 kb on - strandat 3.259 kb on - strandat 3.316 kb on - strandat 3.332 kb on - strandat 3.446 kb on + strand, within Psest_0004at 3.446 kb on + strand, within Psest_0004at 3.447 kb on - strand, within Psest_0004at 3.447 kb on - strand, within Psest_0004at 3.447 kb on - strand, within Psest_0004at 3.447 kb on - strand, within Psest_0004at 3.447 kb on - strand, within Psest_0004at 3.447 kb on - strand, within Psest_0004at 3.540 kb on + strand, within Psest_0004at 3.540 kb on + strand, within Psest_0004at 3.540 kb on + strand, within Psest_0004at 3.541 kb on - strand, within Psest_0004at 3.541 kb on - strand, within Psest_0004at 3.541 kb on - strand, within Psest_0004at 3.613 kb on + strand, within Psest_0004at 3.622 kb on + strand, within Psest_0004at 3.622 kb on + strand, within Psest_0004at 3.622 kb on + strand, within Psest_0004at 3.766 kb on + strand, within Psest_0004at 3.766 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.849 kb on - strand, within Psest_0004at 3.923 kb on + strand, within Psest_0004at 3.923 kb on + strand, within Psest_0004at 3.923 kb on + strand, within Psest_0004at 4.004 kb on - strand, within Psest_0004at 4.107 kb on + strand, within Psest_0004at 4.107 kb on + strand, within Psest_0004at 4.107 kb on + strand, within Psest_0004at 4.111 kb on + strand, within Psest_0004at 4.111 kb on + strand, within Psest_0004at 4.111 kb on + strand, within Psest_0004at 4.111 kb on + strand, within Psest_0004at 4.111 kb on + strand, within Psest_0004at 4.112 kb on - strand, within Psest_0004at 4.160 kb on + strand, within Psest_0004at 4.160 kb on + strand, within Psest_0004

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.0016 mg/ml
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3,245 - -4.3
3,258 + -1.0
3,258 + -1.4
3,258 + -0.6
3,258 + -2.6
3,258 + -1.8
3,259 - -3.2
3,259 - -2.6
3,316 - -1.0
3,332 - -1.2
3,446 + Psest_0004 0.17 -2.8
3,446 + Psest_0004 0.17 -1.2
3,447 - Psest_0004 0.17 -3.5
3,447 - Psest_0004 0.17 -2.5
3,447 - Psest_0004 0.17 -1.7
3,447 - Psest_0004 0.17 -0.9
3,447 - Psest_0004 0.17 -4.1
3,447 - Psest_0004 0.17 -1.8
3,540 + Psest_0004 0.25 -3.4
3,540 + Psest_0004 0.25 -3.0
3,540 + Psest_0004 0.25 -4.5
3,541 - Psest_0004 0.25 -1.6
3,541 - Psest_0004 0.25 -3.3
3,541 - Psest_0004 0.25 -3.2
3,613 + Psest_0004 0.32 -2.3
3,622 + Psest_0004 0.33 -1.9
3,622 + Psest_0004 0.33 -2.7
3,622 + Psest_0004 0.33 -2.9
3,766 + Psest_0004 0.45 -4.7
3,766 + Psest_0004 0.45 -1.8
3,848 + Psest_0004 0.53 -2.6
3,848 + Psest_0004 0.53 -2.4
3,848 + Psest_0004 0.53 -0.4
3,848 + Psest_0004 0.53 -2.1
3,848 + Psest_0004 0.53 -3.1
3,848 + Psest_0004 0.53 -3.7
3,848 + Psest_0004 0.53 -2.8
3,848 + Psest_0004 0.53 -2.2
3,849 - Psest_0004 0.53 -1.4
3,923 + Psest_0004 0.60 -1.4
3,923 + Psest_0004 0.60 -1.4
3,923 + Psest_0004 0.60 -1.7
4,004 - Psest_0004 0.67 -1.2
4,107 + Psest_0004 0.76 -4.2
4,107 + Psest_0004 0.76 -1.7
4,107 + Psest_0004 0.76 -4.9
4,111 + Psest_0004 0.76 -3.1
4,111 + Psest_0004 0.76 -3.3
4,111 + Psest_0004 0.76 -2.3
4,111 + Psest_0004 0.76 -1.9
4,111 + Psest_0004 0.76 -4.2
4,112 - Psest_0004 0.77 -0.0
4,160 + Psest_0004 0.81 -3.3
4,160 + Psest_0004 0.81 -3.2

Or see this region's nucleotide sequence