Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0465 and Psest_0466 are separated by 71 nucleotides Psest_0466 and Psest_0467 are separated by 34 nucleotides
Psest_0465: Psest_0465 - Acetyltransferase (isoleucine patch superfamily), at 512,893 to 513,597
_0465
Psest_0466: Psest_0466 - Lipopolysaccharide kinase (Kdo/WaaP) family., at 513,669 to 514,301
_0466
Psest_0467: Psest_0467 - lipid A export permease/ATP-binding protein MsbA, at 514,336 to 516,153
_0467
Position (kb)
513
514
515 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 512.713 kb on + strand at 512.713 kb on + strand at 512.714 kb on - strand at 512.814 kb on + strand at 512.814 kb on + strand at 512.815 kb on - strand at 512.842 kb on - strand at 512.951 kb on + strand at 512.951 kb on + strand at 512.951 kb on + strand at 512.952 kb on - strand at 512.958 kb on - strand at 513.056 kb on + strand, within Psest_0465 at 513.064 kb on + strand, within Psest_0465 at 513.064 kb on + strand, within Psest_0465 at 513.066 kb on + strand, within Psest_0465 at 513.066 kb on + strand, within Psest_0465 at 513.066 kb on + strand, within Psest_0465 at 513.066 kb on + strand, within Psest_0465 at 513.066 kb on + strand, within Psest_0465 at 513.066 kb on + strand, within Psest_0465 at 513.067 kb on - strand, within Psest_0465 at 513.067 kb on - strand, within Psest_0465 at 513.067 kb on - strand, within Psest_0465 at 513.067 kb on - strand, within Psest_0465 at 513.070 kb on + strand, within Psest_0465 at 513.071 kb on - strand, within Psest_0465 at 513.071 kb on - strand, within Psest_0465 at 513.072 kb on + strand, within Psest_0465 at 513.072 kb on + strand, within Psest_0465 at 513.072 kb on + strand, within Psest_0465 at 513.072 kb on + strand, within Psest_0465 at 513.073 kb on - strand, within Psest_0465 at 513.107 kb on + strand, within Psest_0465 at 513.107 kb on + strand, within Psest_0465 at 513.107 kb on + strand, within Psest_0465 at 513.107 kb on + strand, within Psest_0465 at 513.108 kb on - strand, within Psest_0465 at 513.108 kb on - strand, within Psest_0465 at 513.111 kb on + strand, within Psest_0465 at 513.111 kb on + strand, within Psest_0465 at 513.111 kb on + strand, within Psest_0465 at 513.111 kb on + strand, within Psest_0465 at 513.112 kb on - strand, within Psest_0465 at 513.112 kb on - strand, within Psest_0465 at 513.112 kb on - strand, within Psest_0465 at 513.201 kb on + strand, within Psest_0465 at 513.202 kb on - strand, within Psest_0465 at 513.216 kb on + strand, within Psest_0465 at 513.216 kb on + strand, within Psest_0465 at 513.216 kb on + strand, within Psest_0465 at 513.216 kb on + strand, within Psest_0465 at 513.216 kb on + strand, within Psest_0465 at 513.216 kb on + strand, within Psest_0465 at 513.217 kb on - strand, within Psest_0465 at 513.217 kb on - strand, within Psest_0465 at 513.220 kb on + strand, within Psest_0465 at 513.220 kb on + strand, within Psest_0465 at 513.220 kb on + strand, within Psest_0465 at 513.220 kb on + strand, within Psest_0465 at 513.220 kb on + strand, within Psest_0465 at 513.220 kb on + strand, within Psest_0465 at 513.220 kb on + strand, within Psest_0465 at 513.220 kb on + strand, within Psest_0465 at 513.221 kb on - strand, within Psest_0465 at 513.221 kb on - strand, within Psest_0465 at 513.221 kb on - strand, within Psest_0465 at 513.221 kb on - strand, within Psest_0465 at 513.221 kb on - strand, within Psest_0465 at 513.221 kb on - strand, within Psest_0465 at 513.221 kb on - strand, within Psest_0465 at 513.221 kb on - strand, within Psest_0465 at 513.248 kb on + strand, within Psest_0465 at 513.248 kb on + strand, within Psest_0465 at 513.249 kb on - strand, within Psest_0465 at 513.260 kb on + strand, within Psest_0465 at 513.260 kb on + strand, within Psest_0465 at 513.260 kb on + strand, within Psest_0465 at 513.260 kb on + strand, within Psest_0465 at 513.260 kb on + strand, within Psest_0465 at 513.260 kb on + strand, within Psest_0465 at 513.260 kb on + strand, within Psest_0465 at 513.260 kb on + strand, within Psest_0465 at 513.261 kb on - strand, within Psest_0465 at 513.261 kb on - strand, within Psest_0465 at 513.261 kb on - strand, within Psest_0465 at 513.261 kb on - strand, within Psest_0465 at 513.261 kb on - strand, within Psest_0465 at 513.261 kb on - strand, within Psest_0465 at 513.261 kb on - strand, within Psest_0465 at 513.261 kb on - strand, within Psest_0465 at 513.261 kb on - strand, within Psest_0465 at 513.266 kb on + strand, within Psest_0465 at 513.266 kb on + strand, within Psest_0465 at 513.267 kb on - strand, within Psest_0465 at 513.267 kb on - strand, within Psest_0465 at 513.297 kb on - strand, within Psest_0465 at 513.319 kb on - strand, within Psest_0465 at 513.325 kb on - strand, within Psest_0465 at 513.339 kb on + strand, within Psest_0465 at 513.340 kb on - strand, within Psest_0465 at 513.342 kb on + strand, within Psest_0465 at 513.350 kb on + strand, within Psest_0465 at 513.351 kb on - strand, within Psest_0465 at 513.351 kb on - strand, within Psest_0465 at 513.351 kb on - strand, within Psest_0465 at 513.358 kb on + strand, within Psest_0465 at 513.394 kb on - strand, within Psest_0465 at 513.477 kb on + strand, within Psest_0465 at 513.511 kb on + strand, within Psest_0465 at 513.513 kb on + strand, within Psest_0465 at 513.553 kb on + strand at 513.555 kb on + strand at 513.615 kb on - strand at 513.873 kb on + strand, within Psest_0466 at 513.960 kb on + strand, within Psest_0466 at 513.960 kb on + strand, within Psest_0466 at 513.993 kb on + strand, within Psest_0466 at 513.993 kb on + strand, within Psest_0466 at 514.023 kb on + strand, within Psest_0466 at 514.027 kb on + strand, within Psest_0466 at 514.027 kb on + strand, within Psest_0466 at 514.027 kb on + strand, within Psest_0466 at 514.185 kb on + strand, within Psest_0466 at 514.185 kb on + strand, within Psest_0466 at 514.185 kb on + strand, within Psest_0466 at 514.248 kb on + strand at 514.275 kb on + strand at 514.290 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 512,713 + +0.9 512,713 + -0.9 512,714 - +0.5 512,814 + +0.8 512,814 + +0.9 512,815 - +0.1 512,842 - +0.3 512,951 + +0.1 512,951 + -0.1 512,951 + +0.9 512,952 - -0.7 512,958 - -2.7 513,056 + Psest_0465 0.23 +1.1 513,064 + Psest_0465 0.24 +0.8 513,064 + Psest_0465 0.24 -2.1 513,066 + Psest_0465 0.25 +0.8 513,066 + Psest_0465 0.25 +0.5 513,066 + Psest_0465 0.25 +0.5 513,066 + Psest_0465 0.25 -1.9 513,066 + Psest_0465 0.25 -0.9 513,066 + Psest_0465 0.25 +0.4 513,067 - Psest_0465 0.25 +0.5 513,067 - Psest_0465 0.25 -1.3 513,067 - Psest_0465 0.25 -2.2 513,067 - Psest_0465 0.25 -1.3 513,070 + Psest_0465 0.25 -0.0 513,071 - Psest_0465 0.25 -1.9 513,071 - Psest_0465 0.25 -0.6 513,072 + Psest_0465 0.25 +0.2 513,072 + Psest_0465 0.25 -0.0 513,072 + Psest_0465 0.25 +1.7 513,072 + Psest_0465 0.25 -0.7 513,073 - Psest_0465 0.26 -0.3 513,107 + Psest_0465 0.30 -1.1 513,107 + Psest_0465 0.30 -0.6 513,107 + Psest_0465 0.30 +0.3 513,107 + Psest_0465 0.30 +0.1 513,108 - Psest_0465 0.30 +1.0 513,108 - Psest_0465 0.30 +1.2 513,111 + Psest_0465 0.31 +0.8 513,111 + Psest_0465 0.31 -0.3 513,111 + Psest_0465 0.31 +0.2 513,111 + Psest_0465 0.31 +0.7 513,112 - Psest_0465 0.31 +0.4 513,112 - Psest_0465 0.31 +0.1 513,112 - Psest_0465 0.31 -1.6 513,201 + Psest_0465 0.44 +0.5 513,202 - Psest_0465 0.44 +0.3 513,216 + Psest_0465 0.46 +0.0 513,216 + Psest_0465 0.46 +0.8 513,216 + Psest_0465 0.46 +1.6 513,216 + Psest_0465 0.46 -2.0 513,216 + Psest_0465 0.46 +0.7 513,216 + Psest_0465 0.46 -1.2 513,217 - Psest_0465 0.46 +1.5 513,217 - Psest_0465 0.46 -1.6 513,220 + Psest_0465 0.46 +0.9 513,220 + Psest_0465 0.46 -0.7 513,220 + Psest_0465 0.46 -1.4 513,220 + Psest_0465 0.46 +0.3 513,220 + Psest_0465 0.46 +0.6 513,220 + Psest_0465 0.46 -1.5 513,220 + Psest_0465 0.46 -0.6 513,220 + Psest_0465 0.46 -0.3 513,221 - Psest_0465 0.47 +0.0 513,221 - Psest_0465 0.47 -0.4 513,221 - Psest_0465 0.47 -1.1 513,221 - Psest_0465 0.47 +1.0 513,221 - Psest_0465 0.47 +0.1 513,221 - Psest_0465 0.47 +1.1 513,221 - Psest_0465 0.47 -0.0 513,221 - Psest_0465 0.47 -1.4 513,248 + Psest_0465 0.50 +0.0 513,248 + Psest_0465 0.50 -0.5 513,249 - Psest_0465 0.50 +0.2 513,260 + Psest_0465 0.52 +0.4 513,260 + Psest_0465 0.52 +1.9 513,260 + Psest_0465 0.52 -0.5 513,260 + Psest_0465 0.52 -0.5 513,260 + Psest_0465 0.52 +1.2 513,260 + Psest_0465 0.52 -0.1 513,260 + Psest_0465 0.52 +0.8 513,260 + Psest_0465 0.52 +0.0 513,261 - Psest_0465 0.52 +1.5 513,261 - Psest_0465 0.52 -1.7 513,261 - Psest_0465 0.52 -1.3 513,261 - Psest_0465 0.52 -1.4 513,261 - Psest_0465 0.52 +0.5 513,261 - Psest_0465 0.52 -1.0 513,261 - Psest_0465 0.52 +0.0 513,261 - Psest_0465 0.52 -2.0 513,261 - Psest_0465 0.52 -0.5 513,266 + Psest_0465 0.53 -0.0 513,266 + Psest_0465 0.53 +0.3 513,267 - Psest_0465 0.53 -0.8 513,267 - Psest_0465 0.53 -1.0 513,297 - Psest_0465 0.57 -1.2 513,319 - Psest_0465 0.60 -0.0 513,325 - Psest_0465 0.61 -1.2 513,339 + Psest_0465 0.63 -0.4 513,340 - Psest_0465 0.63 -2.5 513,342 + Psest_0465 0.64 +0.7 513,350 + Psest_0465 0.65 -1.4 513,351 - Psest_0465 0.65 -0.7 513,351 - Psest_0465 0.65 -1.3 513,351 - Psest_0465 0.65 +1.3 513,358 + Psest_0465 0.66 +1.9 513,394 - Psest_0465 0.71 -1.2 513,477 + Psest_0465 0.83 +0.0 513,511 + Psest_0465 0.88 -0.6 513,513 + Psest_0465 0.88 -0.5 513,553 + +0.1 513,555 + -0.7 513,615 - +0.8 513,873 + Psest_0466 0.32 +1.3 513,960 + Psest_0466 0.46 +1.8 513,960 + Psest_0466 0.46 +2.4 513,993 + Psest_0466 0.51 +0.7 513,993 + Psest_0466 0.51 +3.4 514,023 + Psest_0466 0.56 +1.1 514,027 + Psest_0466 0.57 +3.6 514,027 + Psest_0466 0.57 +2.8 514,027 + Psest_0466 0.57 +3.0 514,185 + Psest_0466 0.82 +0.4 514,185 + Psest_0466 0.82 +0.8 514,185 + Psest_0466 0.82 +1.2 514,248 + +0.2 514,275 + +1.9 514,290 + +2.5
Or see this region's nucleotide sequence