Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3935 and Psest_3936 are separated by 137 nucleotides Psest_3936 and Psest_3937 are separated by 90 nucleotides Psest_3937 and Psest_3938 are separated by 47 nucleotides Psest_3938 and Psest_3939 are separated by 155 nucleotides
Psest_3935: Psest_3935 - oxidoreductase alpha (molybdopterin) subunit, at 4,106,281 to 4,108,653
_3935
Psest_3936: Psest_3936 - ATP-dependent protease HslVU, peptidase subunit, at 4,108,791 to 4,109,327
_3936
Psest_3937: Psest_3937 - ATP-dependent protease HslVU, ATPase subunit, at 4,109,418 to 4,110,761
_3937
Psest_3938: Psest_3938 - Uncharacterized protein conserved in bacteria, at 4,110,809 to 4,111,183
_3938
Psest_3939: Psest_3939 - putative polyhydroxyalkanoic acid system protein, at 4,111,339 to 4,111,614
_3939
Position (kb)
4109
4110
4111 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4108.447 kb on + strand at 4108.447 kb on + strand at 4108.447 kb on + strand at 4108.447 kb on + strand at 4108.448 kb on - strand at 4108.448 kb on - strand at 4108.477 kb on + strand at 4108.683 kb on + strand at 4108.684 kb on - strand at 4108.742 kb on + strand at 4108.906 kb on - strand, within Psest_3936 at 4108.906 kb on - strand, within Psest_3936 at 4108.906 kb on - strand, within Psest_3936 at 4108.906 kb on - strand, within Psest_3936 at 4108.919 kb on - strand, within Psest_3936 at 4108.919 kb on - strand, within Psest_3936 at 4109.303 kb on - strand at 4109.303 kb on - strand at 4109.362 kb on + strand at 4109.363 kb on - strand at 4109.686 kb on - strand, within Psest_3937 at 4110.216 kb on - strand, within Psest_3937 at 4110.480 kb on + strand, within Psest_3937 at 4110.699 kb on + strand at 4110.700 kb on - strand at 4110.751 kb on - strand at 4110.751 kb on - strand at 4110.751 kb on - strand at 4110.751 kb on - strand at 4110.751 kb on - strand at 4111.058 kb on - strand, within Psest_3938 at 4111.058 kb on - strand, within Psest_3938 at 4111.058 kb on - strand, within Psest_3938 at 4111.068 kb on - strand, within Psest_3938 at 4111.112 kb on + strand, within Psest_3938 at 4111.112 kb on + strand, within Psest_3938 at 4111.113 kb on - strand, within Psest_3938 at 4111.113 kb on - strand, within Psest_3938 at 4111.113 kb on - strand, within Psest_3938 at 4111.275 kb on - strand at 4111.275 kb on - strand at 4111.275 kb on - strand at 4111.293 kb on + strand at 4111.294 kb on - strand at 4111.294 kb on - strand at 4111.294 kb on - strand at 4111.294 kb on - strand at 4111.307 kb on + strand at 4111.324 kb on - strand at 4111.445 kb on + strand, within Psest_3939 at 4111.446 kb on - strand, within Psest_3939 at 4111.446 kb on - strand, within Psest_3939 at 4111.616 kb on - strand at 4111.649 kb on + strand at 4111.650 kb on - strand at 4111.650 kb on - strand at 4111.652 kb on - strand at 4111.652 kb on - strand at 4111.652 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 4,108,447 + +0.8 4,108,447 + +0.9 4,108,447 + +0.3 4,108,447 + +0.2 4,108,448 - +0.5 4,108,448 - +0.7 4,108,477 + -1.5 4,108,683 + +2.0 4,108,684 - +2.3 4,108,742 + +0.3 4,108,906 - Psest_3936 0.21 +0.9 4,108,906 - Psest_3936 0.21 +3.1 4,108,906 - Psest_3936 0.21 +1.8 4,108,906 - Psest_3936 0.21 +2.3 4,108,919 - Psest_3936 0.24 +2.6 4,108,919 - Psest_3936 0.24 +1.1 4,109,303 - +0.9 4,109,303 - +3.1 4,109,362 + +1.4 4,109,363 - +2.2 4,109,686 - Psest_3937 0.20 +1.5 4,110,216 - Psest_3937 0.59 +1.8 4,110,480 + Psest_3937 0.79 +2.3 4,110,699 + +1.6 4,110,700 - +2.3 4,110,751 - +1.1 4,110,751 - +0.6 4,110,751 - +1.4 4,110,751 - +1.6 4,110,751 - +1.3 4,111,058 - Psest_3938 0.66 -0.7 4,111,058 - Psest_3938 0.66 -0.9 4,111,058 - Psest_3938 0.66 +0.1 4,111,068 - Psest_3938 0.69 -1.7 4,111,112 + Psest_3938 0.81 +0.5 4,111,112 + Psest_3938 0.81 +0.3 4,111,113 - Psest_3938 0.81 -1.3 4,111,113 - Psest_3938 0.81 -2.7 4,111,113 - Psest_3938 0.81 +1.5 4,111,275 - -0.7 4,111,275 - -0.2 4,111,275 - -0.9 4,111,293 + -0.5 4,111,294 - -2.2 4,111,294 - +0.6 4,111,294 - -1.2 4,111,294 - +2.2 4,111,307 + +0.3 4,111,324 - +0.1 4,111,445 + Psest_3939 0.38 -2.9 4,111,446 - Psest_3939 0.39 +0.5 4,111,446 - Psest_3939 0.39 +2.0 4,111,616 - +1.3 4,111,649 + -0.5 4,111,650 - +0.8 4,111,650 - -1.4 4,111,652 - +0.9 4,111,652 - -0.4 4,111,652 - +1.5
Or see this region's nucleotide sequence