Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3699 and Psest_3700 are separated by 10 nucleotides Psest_3700 and Psest_3701 overlap by 4 nucleotides Psest_3701 and Psest_3702 overlap by 4 nucleotides Psest_3702 and Psest_3703 overlap by 4 nucleotides
Psest_3699: Psest_3699 - Dehydrogenases (flavoproteins), at 3,863,619 to 3,864,869
_3699
Psest_3700: Psest_3700 - hypothetical protein, at 3,864,880 to 3,865,386
_3700
Psest_3701: Psest_3701 - 3-oxoacyl-(acyl-carrier-protein) synthase, at 3,865,383 to 3,866,576
_3701
Psest_3702: Psest_3702 - Predicted 3-hydroxylacyl-(acyl carrier protein) dehydratase, at 3,866,573 to 3,867,025
_3702
Psest_3703: Psest_3703 - 3-oxoacyl-(acyl-carrier-protein) reductase, putative, at 3,867,022 to 3,867,750
_3703
Position (kb)
3865
3866
3867 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3864.665 kb on + strand, within Psest_3699 at 3864.678 kb on + strand, within Psest_3699 at 3864.678 kb on + strand, within Psest_3699 at 3864.678 kb on + strand, within Psest_3699 at 3864.678 kb on + strand, within Psest_3699 at 3864.678 kb on + strand, within Psest_3699 at 3864.679 kb on - strand, within Psest_3699 at 3864.679 kb on - strand, within Psest_3699 at 3864.679 kb on - strand, within Psest_3699 at 3864.679 kb on - strand, within Psest_3699 at 3864.679 kb on - strand, within Psest_3699 at 3864.679 kb on - strand, within Psest_3699 at 3864.694 kb on - strand, within Psest_3699 at 3864.720 kb on + strand, within Psest_3699 at 3864.720 kb on + strand, within Psest_3699 at 3864.723 kb on + strand, within Psest_3699 at 3864.723 kb on + strand, within Psest_3699 at 3864.724 kb on - strand, within Psest_3699 at 3864.802 kb on - strand at 3864.802 kb on - strand at 3865.295 kb on - strand, within Psest_3700 at 3865.343 kb on - strand at 3865.343 kb on - strand at 3865.392 kb on + strand at 3865.689 kb on - strand, within Psest_3701 at 3865.834 kb on - strand, within Psest_3701 at 3865.834 kb on - strand, within Psest_3701 at 3866.129 kb on + strand, within Psest_3701 at 3866.130 kb on - strand, within Psest_3701 at 3866.130 kb on - strand, within Psest_3701 at 3866.130 kb on - strand, within Psest_3701 at 3866.130 kb on - strand, within Psest_3701 at 3866.221 kb on - strand, within Psest_3701 at 3866.492 kb on + strand at 3866.492 kb on + strand at 3866.546 kb on + strand at 3866.601 kb on + strand at 3866.834 kb on + strand, within Psest_3702 at 3866.834 kb on + strand, within Psest_3702 at 3866.835 kb on - strand, within Psest_3702 at 3866.835 kb on - strand, within Psest_3702 at 3866.875 kb on - strand, within Psest_3702 at 3867.002 kb on + strand at 3867.003 kb on - strand at 3867.003 kb on - strand at 3867.003 kb on - strand at 3867.006 kb on + strand at 3867.006 kb on + strand at 3867.006 kb on + strand at 3867.006 kb on + strand at 3867.006 kb on + strand at 3867.006 kb on + strand at 3867.006 kb on + strand at 3867.007 kb on - strand at 3867.007 kb on - strand at 3867.007 kb on - strand at 3867.007 kb on - strand at 3867.007 kb on - strand at 3867.007 kb on - strand at 3867.007 kb on - strand at 3867.007 kb on - strand at 3867.161 kb on + strand, within Psest_3703 at 3867.161 kb on + strand, within Psest_3703 at 3867.161 kb on + strand, within Psest_3703 at 3867.161 kb on + strand, within Psest_3703 at 3867.161 kb on + strand, within Psest_3703 at 3867.262 kb on + strand, within Psest_3703 at 3867.263 kb on - strand, within Psest_3703 at 3867.263 kb on - strand, within Psest_3703 at 3867.266 kb on - strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.367 kb on + strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.368 kb on - strand, within Psest_3703 at 3867.478 kb on + strand, within Psest_3703 at 3867.478 kb on + strand, within Psest_3703 at 3867.478 kb on + strand, within Psest_3703 at 3867.478 kb on + strand, within Psest_3703 at 3867.478 kb on + strand, within Psest_3703 at 3867.478 kb on + strand, within Psest_3703 at 3867.478 kb on + strand, within Psest_3703 at 3867.478 kb on + strand, within Psest_3703 at 3867.479 kb on - strand, within Psest_3703 at 3867.479 kb on - strand, within Psest_3703 at 3867.479 kb on - strand, within Psest_3703 at 3867.479 kb on - strand, within Psest_3703 at 3867.479 kb on - strand, within Psest_3703 at 3867.479 kb on - strand, within Psest_3703 at 3867.479 kb on - strand, within Psest_3703 at 3867.479 kb on - strand, within Psest_3703 at 3867.479 kb on - strand, within Psest_3703 at 3867.479 kb on - strand, within Psest_3703
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 3,864,665 + Psest_3699 0.84 +0.1 3,864,678 + Psest_3699 0.85 -1.6 3,864,678 + Psest_3699 0.85 +1.3 3,864,678 + Psest_3699 0.85 +0.7 3,864,678 + Psest_3699 0.85 +0.1 3,864,678 + Psest_3699 0.85 +1.5 3,864,679 - Psest_3699 0.85 -1.5 3,864,679 - Psest_3699 0.85 -1.0 3,864,679 - Psest_3699 0.85 -0.6 3,864,679 - Psest_3699 0.85 +0.7 3,864,679 - Psest_3699 0.85 +0.3 3,864,679 - Psest_3699 0.85 +0.2 3,864,694 - Psest_3699 0.86 +1.4 3,864,720 + Psest_3699 0.88 +1.1 3,864,720 + Psest_3699 0.88 +0.1 3,864,723 + Psest_3699 0.88 -0.7 3,864,723 + Psest_3699 0.88 +0.2 3,864,724 - Psest_3699 0.88 +0.2 3,864,802 - +0.5 3,864,802 - -0.1 3,865,295 - Psest_3700 0.82 -1.1 3,865,343 - -2.1 3,865,343 - -0.3 3,865,392 + +0.3 3,865,689 - Psest_3701 0.26 +0.6 3,865,834 - Psest_3701 0.38 +1.1 3,865,834 - Psest_3701 0.38 +0.5 3,866,129 + Psest_3701 0.62 +2.9 3,866,130 - Psest_3701 0.63 +0.1 3,866,130 - Psest_3701 0.63 +1.5 3,866,130 - Psest_3701 0.63 +0.4 3,866,130 - Psest_3701 0.63 -0.9 3,866,221 - Psest_3701 0.70 -0.9 3,866,492 + -1.2 3,866,492 + -0.1 3,866,546 + -0.9 3,866,601 + +0.5 3,866,834 + Psest_3702 0.58 -0.4 3,866,834 + Psest_3702 0.58 +0.4 3,866,835 - Psest_3702 0.58 -1.5 3,866,835 - Psest_3702 0.58 -0.8 3,866,875 - Psest_3702 0.67 -0.4 3,867,002 + -0.6 3,867,003 - -0.1 3,867,003 - -1.3 3,867,003 - -1.6 3,867,006 + +2.1 3,867,006 + -1.5 3,867,006 + -0.8 3,867,006 + +0.8 3,867,006 + +1.3 3,867,006 + +1.4 3,867,006 + -0.8 3,867,007 - +1.0 3,867,007 - -0.1 3,867,007 - -1.7 3,867,007 - +0.8 3,867,007 - +0.5 3,867,007 - -2.0 3,867,007 - +0.3 3,867,007 - +1.0 3,867,161 + Psest_3703 0.19 +2.3 3,867,161 + Psest_3703 0.19 +0.3 3,867,161 + Psest_3703 0.19 +0.7 3,867,161 + Psest_3703 0.19 -1.1 3,867,161 + Psest_3703 0.19 +0.8 3,867,262 + Psest_3703 0.33 +1.1 3,867,263 - Psest_3703 0.33 +0.4 3,867,263 - Psest_3703 0.33 +0.6 3,867,266 - Psest_3703 0.33 +0.4 3,867,367 + Psest_3703 0.47 +0.8 3,867,367 + Psest_3703 0.47 -1.9 3,867,367 + Psest_3703 0.47 -0.4 3,867,367 + Psest_3703 0.47 +0.2 3,867,367 + Psest_3703 0.47 -1.8 3,867,367 + Psest_3703 0.47 -0.3 3,867,367 + Psest_3703 0.47 +0.1 3,867,367 + Psest_3703 0.47 +0.8 3,867,367 + Psest_3703 0.47 -0.8 3,867,367 + Psest_3703 0.47 -0.2 3,867,367 + Psest_3703 0.47 +0.3 3,867,367 + Psest_3703 0.47 +1.8 3,867,367 + Psest_3703 0.47 +0.2 3,867,367 + Psest_3703 0.47 +0.5 3,867,367 + Psest_3703 0.47 -0.8 3,867,367 + Psest_3703 0.47 -0.1 3,867,368 - Psest_3703 0.47 -0.1 3,867,368 - Psest_3703 0.47 +1.7 3,867,368 - Psest_3703 0.47 -0.7 3,867,368 - Psest_3703 0.47 -0.1 3,867,368 - Psest_3703 0.47 +0.6 3,867,368 - Psest_3703 0.47 -0.2 3,867,368 - Psest_3703 0.47 +2.1 3,867,368 - Psest_3703 0.47 +0.1 3,867,368 - Psest_3703 0.47 -2.0 3,867,368 - Psest_3703 0.47 +1.9 3,867,368 - Psest_3703 0.47 +0.1 3,867,368 - Psest_3703 0.47 -0.5 3,867,368 - Psest_3703 0.47 +1.4 3,867,368 - Psest_3703 0.47 +0.3 3,867,368 - Psest_3703 0.47 +0.4 3,867,368 - Psest_3703 0.47 +1.7 3,867,368 - Psest_3703 0.47 +0.5 3,867,368 - Psest_3703 0.47 -0.1 3,867,368 - Psest_3703 0.47 -0.5 3,867,368 - Psest_3703 0.47 -0.1 3,867,368 - Psest_3703 0.47 -0.2 3,867,478 + Psest_3703 0.63 -0.9 3,867,478 + Psest_3703 0.63 -0.9 3,867,478 + Psest_3703 0.63 -0.3 3,867,478 + Psest_3703 0.63 +0.3 3,867,478 + Psest_3703 0.63 +0.3 3,867,478 + Psest_3703 0.63 -0.4 3,867,478 + Psest_3703 0.63 +0.1 3,867,478 + Psest_3703 0.63 -1.7 3,867,479 - Psest_3703 0.63 +0.3 3,867,479 - Psest_3703 0.63 -1.8 3,867,479 - Psest_3703 0.63 -0.9 3,867,479 - Psest_3703 0.63 +1.0 3,867,479 - Psest_3703 0.63 -0.8 3,867,479 - Psest_3703 0.63 +1.2 3,867,479 - Psest_3703 0.63 -0.5 3,867,479 - Psest_3703 0.63 -0.9 3,867,479 - Psest_3703 0.63 -0.1 3,867,479 - Psest_3703 0.63 -0.5
Or see this region's nucleotide sequence