Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3353 and Psest_3354 are separated by 20 nucleotides Psest_3354 and Psest_3355 overlap by 1 nucleotides
Psest_3353: Psest_3353 - PTS system, fructose-specific, IIB component/PTS system, fructose subfamily, IIC component, at 3,521,685 to 3,523,424
_3353
Psest_3354: Psest_3354 - 1-phosphofructokinase (EC 2.7.1.56) (from data) , at 3,523,445 to 3,524,383
_3354
Psest_3355: Psest_3355 - fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components (from data) , at 3,524,383 to 3,527,265
_3355
Position (kb)
3523
3524
3525 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3522.512 kb on - strand, within Psest_3353 at 3522.512 kb on - strand, within Psest_3353 at 3522.520 kb on + strand, within Psest_3353 at 3522.520 kb on + strand, within Psest_3353 at 3522.676 kb on + strand, within Psest_3353 at 3522.676 kb on + strand, within Psest_3353 at 3522.676 kb on + strand, within Psest_3353 at 3522.676 kb on + strand, within Psest_3353 at 3522.676 kb on + strand, within Psest_3353 at 3522.677 kb on - strand, within Psest_3353 at 3522.677 kb on - strand, within Psest_3353 at 3522.677 kb on - strand, within Psest_3353 at 3522.677 kb on - strand, within Psest_3353 at 3522.677 kb on - strand, within Psest_3353 at 3522.703 kb on + strand, within Psest_3353 at 3522.703 kb on + strand, within Psest_3353 at 3522.703 kb on + strand, within Psest_3353 at 3522.703 kb on + strand, within Psest_3353 at 3522.759 kb on - strand, within Psest_3353 at 3522.939 kb on - strand, within Psest_3353 at 3523.085 kb on + strand, within Psest_3353 at 3523.086 kb on - strand, within Psest_3353 at 3523.446 kb on + strand at 3523.885 kb on + strand, within Psest_3354 at 3523.928 kb on + strand, within Psest_3354 at 3523.928 kb on + strand, within Psest_3354 at 3524.279 kb on + strand, within Psest_3354 at 3524.279 kb on + strand, within Psest_3354 at 3524.279 kb on + strand, within Psest_3354 at 3524.279 kb on + strand, within Psest_3354 at 3524.279 kb on + strand, within Psest_3354 at 3524.279 kb on + strand, within Psest_3354 at 3524.279 kb on + strand, within Psest_3354 at 3524.279 kb on + strand, within Psest_3354 at 3524.279 kb on + strand, within Psest_3354 at 3524.280 kb on - strand, within Psest_3354 at 3524.889 kb on + strand, within Psest_3355
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 3,522,512 - Psest_3353 0.48 -1.2 3,522,512 - Psest_3353 0.48 -1.1 3,522,520 + Psest_3353 0.48 -0.6 3,522,520 + Psest_3353 0.48 +0.4 3,522,676 + Psest_3353 0.57 +0.6 3,522,676 + Psest_3353 0.57 +0.6 3,522,676 + Psest_3353 0.57 -0.8 3,522,676 + Psest_3353 0.57 +2.3 3,522,676 + Psest_3353 0.57 -0.6 3,522,677 - Psest_3353 0.57 +1.0 3,522,677 - Psest_3353 0.57 -0.4 3,522,677 - Psest_3353 0.57 +1.9 3,522,677 - Psest_3353 0.57 -1.5 3,522,677 - Psest_3353 0.57 +1.6 3,522,703 + Psest_3353 0.59 +0.7 3,522,703 + Psest_3353 0.59 -0.2 3,522,703 + Psest_3353 0.59 -1.2 3,522,703 + Psest_3353 0.59 -0.9 3,522,759 - Psest_3353 0.62 +0.4 3,522,939 - Psest_3353 0.72 +0.2 3,523,085 + Psest_3353 0.80 +0.5 3,523,086 - Psest_3353 0.81 -0.0 3,523,446 + -0.9 3,523,885 + Psest_3354 0.47 +2.5 3,523,928 + Psest_3354 0.51 -0.2 3,523,928 + Psest_3354 0.51 +2.3 3,524,279 + Psest_3354 0.89 -1.5 3,524,279 + Psest_3354 0.89 +1.9 3,524,279 + Psest_3354 0.89 +1.2 3,524,279 + Psest_3354 0.89 -0.9 3,524,279 + Psest_3354 0.89 +1.3 3,524,279 + Psest_3354 0.89 +1.0 3,524,279 + Psest_3354 0.89 +1.8 3,524,279 + Psest_3354 0.89 +1.2 3,524,279 + Psest_3354 0.89 +0.7 3,524,280 - Psest_3354 0.89 +3.1 3,524,889 + Psest_3355 0.18 -1.3
Or see this region's nucleotide sequence