Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3178 and Psest_3179 are separated by 351 nucleotides Psest_3179 and Psest_3180 are separated by 144 nucleotides
Psest_3178: Psest_3178 - formyltetrahydrofolate deformylase, at 3,349,903 to 3,350,754
_3178
Psest_3179: Psest_3179 - hypothetical protein, at 3,351,106 to 3,351,486
_3179
Psest_3180: Psest_3180 - Exonuclease I, at 3,351,631 to 3,353,091
_3180
Position (kb)
3351
3352 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3350.430 kb on + strand, within Psest_3178 at 3350.675 kb on + strand at 3350.675 kb on + strand at 3350.675 kb on + strand at 3350.849 kb on + strand at 3350.929 kb on + strand at 3350.929 kb on + strand at 3350.972 kb on + strand at 3351.033 kb on + strand at 3351.035 kb on + strand at 3351.039 kb on + strand at 3351.039 kb on + strand at 3351.060 kb on + strand at 3351.689 kb on - strand at 3351.990 kb on - strand, within Psest_3180 at 3351.990 kb on - strand, within Psest_3180 at 3352.133 kb on - strand, within Psest_3180 at 3352.133 kb on - strand, within Psest_3180 at 3352.133 kb on - strand, within Psest_3180 at 3352.133 kb on - strand, within Psest_3180 at 3352.133 kb on - strand, within Psest_3180 at 3352.190 kb on - strand, within Psest_3180 at 3352.190 kb on - strand, within Psest_3180 at 3352.190 kb on - strand, within Psest_3180 at 3352.242 kb on + strand, within Psest_3180 at 3352.242 kb on + strand, within Psest_3180 at 3352.244 kb on + strand, within Psest_3180 at 3352.244 kb on + strand, within Psest_3180 at 3352.245 kb on - strand, within Psest_3180 at 3352.245 kb on - strand, within Psest_3180 at 3352.245 kb on - strand, within Psest_3180 at 3352.245 kb on - strand, within Psest_3180 at 3352.245 kb on - strand, within Psest_3180 at 3352.245 kb on - strand, within Psest_3180 at 3352.245 kb on - strand, within Psest_3180 at 3352.245 kb on - strand, within Psest_3180 at 3352.246 kb on + strand, within Psest_3180 at 3352.247 kb on - strand, within Psest_3180 at 3352.247 kb on - strand, within Psest_3180 at 3352.247 kb on - strand, within Psest_3180 at 3352.247 kb on - strand, within Psest_3180 at 3352.247 kb on - strand, within Psest_3180 at 3352.247 kb on - strand, within Psest_3180 at 3352.247 kb on - strand, within Psest_3180 at 3352.463 kb on + strand, within Psest_3180 at 3352.464 kb on - strand, within Psest_3180 at 3352.464 kb on - strand, within Psest_3180 at 3352.464 kb on - strand, within Psest_3180 at 3352.464 kb on - strand, within Psest_3180 at 3352.464 kb on - strand, within Psest_3180 at 3352.464 kb on - strand, within Psest_3180 at 3352.464 kb on - strand, within Psest_3180 at 3352.470 kb on - strand, within Psest_3180 at 3352.470 kb on - strand, within Psest_3180 at 3352.470 kb on - strand, within Psest_3180 at 3352.470 kb on - strand, within Psest_3180 at 3352.476 kb on - strand, within Psest_3180 at 3352.476 kb on - strand, within Psest_3180
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 3,350,430 + Psest_3178 0.62 +2.1 3,350,675 + +1.9 3,350,675 + +1.1 3,350,675 + +1.3 3,350,849 + +1.2 3,350,929 + +2.8 3,350,929 + +1.6 3,350,972 + +1.1 3,351,033 + +1.1 3,351,035 + +1.9 3,351,039 + -0.0 3,351,039 + +0.4 3,351,060 + -2.0 3,351,689 - -0.6 3,351,990 - Psest_3180 0.25 -2.1 3,351,990 - Psest_3180 0.25 +0.0 3,352,133 - Psest_3180 0.34 -2.1 3,352,133 - Psest_3180 0.34 -0.9 3,352,133 - Psest_3180 0.34 -1.2 3,352,133 - Psest_3180 0.34 +0.4 3,352,133 - Psest_3180 0.34 +0.2 3,352,190 - Psest_3180 0.38 -1.5 3,352,190 - Psest_3180 0.38 +1.5 3,352,190 - Psest_3180 0.38 -3.3 3,352,242 + Psest_3180 0.42 -1.6 3,352,242 + Psest_3180 0.42 -1.0 3,352,244 + Psest_3180 0.42 -2.2 3,352,244 + Psest_3180 0.42 -1.2 3,352,245 - Psest_3180 0.42 -0.7 3,352,245 - Psest_3180 0.42 -0.5 3,352,245 - Psest_3180 0.42 -1.1 3,352,245 - Psest_3180 0.42 +0.1 3,352,245 - Psest_3180 0.42 +0.1 3,352,245 - Psest_3180 0.42 -0.3 3,352,245 - Psest_3180 0.42 +0.6 3,352,245 - Psest_3180 0.42 -1.2 3,352,246 + Psest_3180 0.42 -1.2 3,352,247 - Psest_3180 0.42 +1.1 3,352,247 - Psest_3180 0.42 -0.4 3,352,247 - Psest_3180 0.42 -1.7 3,352,247 - Psest_3180 0.42 -1.0 3,352,247 - Psest_3180 0.42 +0.0 3,352,247 - Psest_3180 0.42 -0.0 3,352,247 - Psest_3180 0.42 -1.2 3,352,463 + Psest_3180 0.57 -1.4 3,352,464 - Psest_3180 0.57 +1.4 3,352,464 - Psest_3180 0.57 -0.7 3,352,464 - Psest_3180 0.57 +1.4 3,352,464 - Psest_3180 0.57 -1.1 3,352,464 - Psest_3180 0.57 -1.6 3,352,464 - Psest_3180 0.57 +0.7 3,352,464 - Psest_3180 0.57 -0.7 3,352,470 - Psest_3180 0.57 +0.8 3,352,470 - Psest_3180 0.57 +1.0 3,352,470 - Psest_3180 0.57 -2.1 3,352,470 - Psest_3180 0.57 +0.4 3,352,476 - Psest_3180 0.58 -3.0 3,352,476 - Psest_3180 0.58 -4.0
Or see this region's nucleotide sequence