Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3174 and Psest_3175 are separated by 319 nucleotides Psest_3175 and Psest_3176 are separated by 77 nucleotides Psest_3176 and Psest_3177 overlap by 1 nucleotides Psest_3177 and Psest_3178 are separated by 15 nucleotides
Psest_3174: Psest_3174 - mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase, at 3,346,136 to 3,347,581
_3174
Psest_3175: Psest_3175 - hypothetical protein, at 3,347,901 to 3,348,122
_3175
Psest_3176: Psest_3176 - Predicted ATPase, at 3,348,200 to 3,349,675
_3176
Psest_3177: Psest_3177 - hypothetical protein, at 3,349,675 to 3,349,887
_3177
Psest_3178: Psest_3178 - formyltetrahydrofolate deformylase, at 3,349,903 to 3,350,754
_3178
Position (kb)
3348
3349
3350 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3347.203 kb on + strand, within Psest_3174 at 3347.204 kb on - strand, within Psest_3174 at 3347.204 kb on - strand, within Psest_3174 at 3347.222 kb on + strand, within Psest_3174 at 3347.223 kb on - strand, within Psest_3174 at 3347.240 kb on + strand, within Psest_3174 at 3347.240 kb on + strand, within Psest_3174 at 3347.254 kb on + strand, within Psest_3174 at 3347.289 kb on + strand, within Psest_3174 at 3347.290 kb on - strand, within Psest_3174 at 3347.417 kb on + strand, within Psest_3174 at 3347.417 kb on + strand, within Psest_3174 at 3347.417 kb on + strand, within Psest_3174 at 3347.417 kb on + strand, within Psest_3174 at 3347.417 kb on + strand, within Psest_3174 at 3347.417 kb on + strand, within Psest_3174 at 3347.417 kb on + strand, within Psest_3174 at 3347.417 kb on + strand, within Psest_3174 at 3347.417 kb on + strand, within Psest_3174 at 3347.417 kb on + strand, within Psest_3174 at 3347.417 kb on + strand, within Psest_3174 at 3347.417 kb on + strand, within Psest_3174 at 3347.418 kb on - strand, within Psest_3174 at 3347.418 kb on - strand, within Psest_3174 at 3347.418 kb on - strand, within Psest_3174 at 3347.418 kb on - strand, within Psest_3174 at 3347.418 kb on - strand, within Psest_3174 at 3347.418 kb on - strand, within Psest_3174 at 3347.418 kb on - strand, within Psest_3174 at 3347.418 kb on - strand, within Psest_3174 at 3347.418 kb on - strand, within Psest_3174 at 3347.418 kb on - strand, within Psest_3174 at 3347.418 kb on - strand, within Psest_3174 at 3347.418 kb on - strand, within Psest_3174 at 3347.418 kb on - strand, within Psest_3174 at 3347.418 kb on - strand, within Psest_3174 at 3347.418 kb on - strand, within Psest_3174 at 3347.429 kb on - strand, within Psest_3174 at 3347.498 kb on + strand at 3347.498 kb on + strand at 3347.498 kb on + strand at 3347.498 kb on + strand at 3347.498 kb on + strand at 3347.498 kb on + strand at 3347.499 kb on - strand at 3347.537 kb on + strand at 3347.538 kb on - strand at 3347.538 kb on - strand at 3347.590 kb on + strand at 3347.590 kb on + strand at 3347.591 kb on - strand at 3347.598 kb on + strand at 3347.664 kb on + strand at 3347.664 kb on + strand at 3347.664 kb on + strand at 3347.664 kb on + strand at 3347.664 kb on + strand at 3347.706 kb on + strand at 3347.706 kb on + strand at 3347.706 kb on + strand at 3347.707 kb on - strand at 3347.707 kb on - strand at 3347.728 kb on - strand at 3347.787 kb on + strand at 3347.871 kb on + strand at 3347.872 kb on - strand at 3347.872 kb on - strand at 3347.873 kb on + strand at 3347.873 kb on + strand at 3347.874 kb on - strand at 3347.874 kb on - strand at 3347.899 kb on - strand at 3347.899 kb on - strand at 3347.983 kb on - strand, within Psest_3175 at 3348.120 kb on + strand at 3348.449 kb on + strand, within Psest_3176 at 3348.500 kb on + strand, within Psest_3176 at 3348.682 kb on + strand, within Psest_3176 at 3348.684 kb on + strand, within Psest_3176 at 3348.684 kb on + strand, within Psest_3176 at 3348.768 kb on + strand, within Psest_3176 at 3348.957 kb on + strand, within Psest_3176 at 3348.958 kb on - strand, within Psest_3176 at 3349.081 kb on + strand, within Psest_3176 at 3349.260 kb on + strand, within Psest_3176 at 3349.336 kb on + strand, within Psest_3176 at 3349.336 kb on + strand, within Psest_3176 at 3349.336 kb on + strand, within Psest_3176 at 3349.337 kb on - strand, within Psest_3176 at 3349.340 kb on + strand, within Psest_3176 at 3349.386 kb on + strand, within Psest_3176 at 3349.451 kb on + strand, within Psest_3176 at 3349.827 kb on + strand, within Psest_3177 at 3349.827 kb on + strand, within Psest_3177 at 3349.828 kb on - strand, within Psest_3177 at 3349.930 kb on - strand at 3349.930 kb on - strand at 3349.990 kb on + strand, within Psest_3178 at 3350.093 kb on + strand, within Psest_3178 at 3350.093 kb on + strand, within Psest_3178 at 3350.093 kb on + strand, within Psest_3178 at 3350.093 kb on + strand, within Psest_3178 at 3350.094 kb on - strand, within Psest_3178 at 3350.430 kb on + strand, within Psest_3178 at 3350.675 kb on + strand at 3350.675 kb on + strand at 3350.675 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 3,347,203 + Psest_3174 0.74 +1.7 3,347,204 - Psest_3174 0.74 +2.4 3,347,204 - Psest_3174 0.74 +2.7 3,347,222 + Psest_3174 0.75 +1.7 3,347,223 - Psest_3174 0.75 +2.1 3,347,240 + Psest_3174 0.76 +1.2 3,347,240 + Psest_3174 0.76 +2.8 3,347,254 + Psest_3174 0.77 +1.5 3,347,289 + Psest_3174 0.80 +2.8 3,347,290 - Psest_3174 0.80 +0.0 3,347,417 + Psest_3174 0.89 +0.3 3,347,417 + Psest_3174 0.89 +1.1 3,347,417 + Psest_3174 0.89 +3.0 3,347,417 + Psest_3174 0.89 -0.0 3,347,417 + Psest_3174 0.89 +2.0 3,347,417 + Psest_3174 0.89 +2.8 3,347,417 + Psest_3174 0.89 +2.3 3,347,417 + Psest_3174 0.89 -1.4 3,347,417 + Psest_3174 0.89 +2.9 3,347,417 + Psest_3174 0.89 +4.1 3,347,417 + Psest_3174 0.89 +2.4 3,347,417 + Psest_3174 0.89 +1.6 3,347,418 - Psest_3174 0.89 +3.4 3,347,418 - Psest_3174 0.89 +0.6 3,347,418 - Psest_3174 0.89 +2.3 3,347,418 - Psest_3174 0.89 +2.3 3,347,418 - Psest_3174 0.89 +0.7 3,347,418 - Psest_3174 0.89 -3.0 3,347,418 - Psest_3174 0.89 +3.4 3,347,418 - Psest_3174 0.89 -0.6 3,347,418 - Psest_3174 0.89 +1.7 3,347,418 - Psest_3174 0.89 +3.0 3,347,418 - Psest_3174 0.89 -0.4 3,347,418 - Psest_3174 0.89 -0.5 3,347,418 - Psest_3174 0.89 +2.9 3,347,418 - Psest_3174 0.89 +2.8 3,347,418 - Psest_3174 0.89 +3.0 3,347,429 - Psest_3174 0.89 +3.2 3,347,498 + +2.3 3,347,498 + +2.7 3,347,498 + +1.7 3,347,498 + +2.2 3,347,498 + +0.9 3,347,498 + +3.8 3,347,499 - +2.2 3,347,537 + +1.4 3,347,538 - +1.5 3,347,538 - -0.6 3,347,590 + -1.0 3,347,590 + -0.0 3,347,591 - +0.5 3,347,598 + -0.9 3,347,664 + +0.4 3,347,664 + +0.9 3,347,664 + +1.9 3,347,664 + -1.8 3,347,664 + -0.7 3,347,706 + -1.4 3,347,706 + +1.1 3,347,706 + +0.6 3,347,707 - +0.6 3,347,707 - +1.5 3,347,728 - -0.4 3,347,787 + -0.8 3,347,871 + -1.2 3,347,872 - -0.0 3,347,872 - +0.6 3,347,873 + -0.5 3,347,873 + -1.1 3,347,874 - -0.4 3,347,874 - -0.4 3,347,899 - -2.1 3,347,899 - -1.8 3,347,983 - Psest_3175 0.37 -1.8 3,348,120 + +0.6 3,348,449 + Psest_3176 0.17 +0.0 3,348,500 + Psest_3176 0.20 -1.1 3,348,682 + Psest_3176 0.33 -0.8 3,348,684 + Psest_3176 0.33 -0.3 3,348,684 + Psest_3176 0.33 -0.6 3,348,768 + Psest_3176 0.38 -1.3 3,348,957 + Psest_3176 0.51 -2.8 3,348,958 - Psest_3176 0.51 -0.4 3,349,081 + Psest_3176 0.60 -0.4 3,349,260 + Psest_3176 0.72 +0.5 3,349,336 + Psest_3176 0.77 +0.5 3,349,336 + Psest_3176 0.77 -0.1 3,349,336 + Psest_3176 0.77 +2.0 3,349,337 - Psest_3176 0.77 +0.0 3,349,340 + Psest_3176 0.77 -0.4 3,349,386 + Psest_3176 0.80 -0.9 3,349,451 + Psest_3176 0.85 +0.0 3,349,827 + Psest_3177 0.71 +1.3 3,349,827 + Psest_3177 0.71 +2.0 3,349,828 - Psest_3177 0.72 -0.6 3,349,930 - -2.2 3,349,930 - +1.3 3,349,990 + Psest_3178 0.10 +1.7 3,350,093 + Psest_3178 0.22 +1.7 3,350,093 + Psest_3178 0.22 +2.7 3,350,093 + Psest_3178 0.22 +1.2 3,350,093 + Psest_3178 0.22 -0.5 3,350,094 - Psest_3178 0.22 -1.3 3,350,430 + Psest_3178 0.62 +2.1 3,350,675 + +1.9 3,350,675 + +1.1 3,350,675 + +1.3
Or see this region's nucleotide sequence