Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3043 and Psest_3044 are separated by 102 nucleotides Psest_3044 and Psest_3045 are separated by 330 nucleotides Psest_3045 and Psest_3046 are separated by 104 nucleotides
Psest_3043: Psest_3043 - periplasmic nitrate (or nitrite) reductase c-type cytochrome, NapC/NirT family, at 3,226,124 to 3,226,720
_3043
Psest_3044: Psest_3044 - deoxycytidine triphosphate deaminase, at 3,226,823 to 3,227,389
_3044
Psest_3045: Psest_3045 - Cold shock proteins, at 3,227,720 to 3,227,929
_3045
Psest_3046: Psest_3046 - ATPases involved in chromosome partitioning, at 3,228,034 to 3,229,128
_3046
Position (kb)
3227
3228 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 3226.729 kb on + strand at 3226.729 kb on + strand at 3226.730 kb on - strand at 3226.730 kb on - strand at 3226.730 kb on - strand at 3226.860 kb on + strand at 3226.861 kb on - strand at 3226.861 kb on - strand at 3226.881 kb on + strand, within Psest_3044 at 3226.944 kb on + strand, within Psest_3044 at 3226.944 kb on + strand, within Psest_3044 at 3226.944 kb on + strand, within Psest_3044 at 3226.944 kb on + strand, within Psest_3044 at 3226.945 kb on - strand, within Psest_3044 at 3226.945 kb on - strand, within Psest_3044 at 3226.945 kb on - strand, within Psest_3044 at 3226.945 kb on - strand, within Psest_3044 at 3226.945 kb on - strand, within Psest_3044 at 3226.945 kb on - strand, within Psest_3044 at 3226.945 kb on - strand, within Psest_3044 at 3227.049 kb on + strand, within Psest_3044 at 3227.050 kb on - strand, within Psest_3044 at 3227.050 kb on - strand, within Psest_3044 at 3227.050 kb on - strand, within Psest_3044 at 3227.100 kb on + strand, within Psest_3044 at 3227.100 kb on + strand, within Psest_3044 at 3227.101 kb on - strand, within Psest_3044 at 3227.257 kb on - strand, within Psest_3044 at 3227.262 kb on + strand, within Psest_3044 at 3227.405 kb on + strand at 3227.406 kb on - strand at 3227.439 kb on - strand at 3227.467 kb on - strand at 3227.532 kb on + strand at 3227.532 kb on + strand at 3227.533 kb on - strand at 3227.560 kb on + strand at 3227.560 kb on + strand at 3227.561 kb on - strand at 3227.566 kb on + strand at 3227.602 kb on + strand at 3227.602 kb on + strand at 3227.603 kb on - strand at 3227.603 kb on - strand at 3227.610 kb on + strand at 3227.611 kb on - strand at 3227.611 kb on - strand at 3227.626 kb on + strand at 3227.642 kb on - strand at 3227.688 kb on - strand at 3227.746 kb on + strand, within Psest_3045 at 3227.811 kb on + strand, within Psest_3045 at 3227.812 kb on - strand, within Psest_3045 at 3227.812 kb on - strand, within Psest_3045 at 3227.923 kb on + strand at 3227.927 kb on + strand at 3227.928 kb on - strand at 3227.928 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 3,226,729 + +0.7 3,226,729 + +1.2 3,226,730 - +1.1 3,226,730 - -1.0 3,226,730 - -0.8 3,226,860 + +2.4 3,226,861 - +0.6 3,226,861 - +0.8 3,226,881 + Psest_3044 0.10 +2.6 3,226,944 + Psest_3044 0.21 +0.2 3,226,944 + Psest_3044 0.21 +0.2 3,226,944 + Psest_3044 0.21 -0.6 3,226,944 + Psest_3044 0.21 +0.6 3,226,945 - Psest_3044 0.22 +1.6 3,226,945 - Psest_3044 0.22 +0.1 3,226,945 - Psest_3044 0.22 +0.8 3,226,945 - Psest_3044 0.22 +1.0 3,226,945 - Psest_3044 0.22 +1.1 3,226,945 - Psest_3044 0.22 +1.5 3,226,945 - Psest_3044 0.22 +0.5 3,227,049 + Psest_3044 0.40 +0.8 3,227,050 - Psest_3044 0.40 +0.8 3,227,050 - Psest_3044 0.40 +0.8 3,227,050 - Psest_3044 0.40 +0.6 3,227,100 + Psest_3044 0.49 -0.4 3,227,100 + Psest_3044 0.49 +1.3 3,227,101 - Psest_3044 0.49 +0.9 3,227,257 - Psest_3044 0.77 -1.4 3,227,262 + Psest_3044 0.77 +0.7 3,227,405 + -0.6 3,227,406 - +1.2 3,227,439 - +0.5 3,227,467 - +1.4 3,227,532 + +1.7 3,227,532 + +1.1 3,227,533 - +1.1 3,227,560 + +1.4 3,227,560 + +2.4 3,227,561 - +2.7 3,227,566 + +3.8 3,227,602 + +1.9 3,227,602 + +2.4 3,227,603 - +1.7 3,227,603 - +3.6 3,227,610 + +0.7 3,227,611 - +3.0 3,227,611 - +3.3 3,227,626 + +1.9 3,227,642 - +0.8 3,227,688 - +1.5 3,227,746 + Psest_3045 0.12 +4.1 3,227,811 + Psest_3045 0.43 +0.2 3,227,812 - Psest_3045 0.44 +4.8 3,227,812 - Psest_3045 0.44 +0.9 3,227,923 + +1.5 3,227,927 + +2.0 3,227,928 - -0.6 3,227,928 - +2.7
Or see this region's nucleotide sequence