Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3020 and Psest_3021 are separated by 67 nucleotides Psest_3021 and Psest_3022 are separated by 134 nucleotides Psest_3022 and Psest_3023 are separated by 5 nucleotides Psest_3023 and Psest_3024 are separated by 48 nucleotides
Psest_3020: Psest_3020 - Cell wall-associated hydrolases (invasion-associated proteins), at 3,208,751 to 3,209,383
_3020
Psest_3021: Psest_3021 - NAD-dependent protein deacetylases, SIR2 family, at 3,209,451 to 3,210,215
_3021
Psest_3022: Psest_3022 - Predicted membrane protein, at 3,210,350 to 3,210,640
_3022
Psest_3023: Psest_3023 - cyd operon protein YbgT, at 3,210,646 to 3,210,750
_3023
Psest_3024: Psest_3024 - cytochrome d oxidase, subunit II (cydB), at 3,210,799 to 3,211,941
_3024
Position (kb)
3210
3211 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3209.475 kb on + strand at 3209.475 kb on + strand at 3209.475 kb on + strand at 3209.475 kb on + strand at 3209.476 kb on - strand at 3209.553 kb on + strand, within Psest_3021 at 3209.553 kb on + strand, within Psest_3021 at 3209.635 kb on - strand, within Psest_3021 at 3210.010 kb on + strand, within Psest_3021 at 3210.010 kb on + strand, within Psest_3021 at 3210.010 kb on + strand, within Psest_3021 at 3210.011 kb on - strand, within Psest_3021 at 3210.097 kb on + strand, within Psest_3021 at 3210.098 kb on - strand, within Psest_3021 at 3210.098 kb on - strand, within Psest_3021 at 3210.098 kb on - strand, within Psest_3021 at 3210.098 kb on - strand, within Psest_3021 at 3210.119 kb on - strand, within Psest_3021 at 3210.210 kb on - strand at 3210.304 kb on + strand at 3210.351 kb on + strand at 3210.351 kb on + strand at 3210.351 kb on + strand at 3210.352 kb on - strand at 3210.352 kb on - strand at 3210.352 kb on - strand at 3210.352 kb on - strand at 3210.352 kb on - strand at 3210.352 kb on - strand at 3210.352 kb on - strand at 3210.352 kb on - strand at 3210.470 kb on + strand, within Psest_3022 at 3210.470 kb on + strand, within Psest_3022 at 3210.470 kb on + strand, within Psest_3022 at 3210.471 kb on - strand, within Psest_3022 at 3210.471 kb on - strand, within Psest_3022 at 3210.471 kb on - strand, within Psest_3022 at 3210.471 kb on - strand, within Psest_3022 at 3210.522 kb on + strand, within Psest_3022 at 3210.523 kb on - strand, within Psest_3022 at 3210.743 kb on + strand at 3210.744 kb on - strand at 3210.744 kb on - strand at 3210.744 kb on - strand at 3210.750 kb on + strand at 3210.750 kb on + strand at 3210.750 kb on + strand at 3210.750 kb on + strand at 3210.750 kb on + strand at 3210.750 kb on + strand at 3210.750 kb on + strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.803 kb on + strand at 3210.804 kb on - strand at 3210.804 kb on - strand at 3210.863 kb on + strand at 3210.864 kb on - strand at 3210.864 kb on - strand at 3210.864 kb on - strand at 3210.864 kb on - strand at 3210.864 kb on - strand at 3210.879 kb on - strand at 3210.940 kb on - strand, within Psest_3024 at 3210.940 kb on - strand, within Psest_3024 at 3211.163 kb on + strand, within Psest_3024 at 3211.163 kb on + strand, within Psest_3024 at 3211.163 kb on + strand, within Psest_3024 at 3211.163 kb on + strand, within Psest_3024 at 3211.164 kb on - strand, within Psest_3024 at 3211.164 kb on - strand, within Psest_3024 at 3211.164 kb on - strand, within Psest_3024 at 3211.164 kb on - strand, within Psest_3024 at 3211.164 kb on - strand, within Psest_3024 at 3211.164 kb on - strand, within Psest_3024 at 3211.312 kb on + strand, within Psest_3024 at 3211.472 kb on + strand, within Psest_3024 at 3211.473 kb on - strand, within Psest_3024
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 3,209,475 + +0.1 3,209,475 + +1.4 3,209,475 + -0.8 3,209,475 + -0.2 3,209,476 - +0.4 3,209,553 + Psest_3021 0.13 -0.2 3,209,553 + Psest_3021 0.13 +2.6 3,209,635 - Psest_3021 0.24 +0.4 3,210,010 + Psest_3021 0.73 -0.4 3,210,010 + Psest_3021 0.73 -1.9 3,210,010 + Psest_3021 0.73 +0.9 3,210,011 - Psest_3021 0.73 -1.1 3,210,097 + Psest_3021 0.84 +0.2 3,210,098 - Psest_3021 0.85 +1.7 3,210,098 - Psest_3021 0.85 +0.4 3,210,098 - Psest_3021 0.85 +0.3 3,210,098 - Psest_3021 0.85 +1.2 3,210,119 - Psest_3021 0.87 +0.2 3,210,210 - +2.5 3,210,304 + +0.8 3,210,351 + +0.0 3,210,351 + +2.4 3,210,351 + +0.1 3,210,352 - +0.6 3,210,352 - -0.0 3,210,352 - -0.5 3,210,352 - +0.1 3,210,352 - +2.2 3,210,352 - +0.9 3,210,352 - -0.2 3,210,352 - -0.4 3,210,470 + Psest_3022 0.41 +4.6 3,210,470 + Psest_3022 0.41 +1.3 3,210,470 + Psest_3022 0.41 -1.5 3,210,471 - Psest_3022 0.42 +2.2 3,210,471 - Psest_3022 0.42 +1.1 3,210,471 - Psest_3022 0.42 +1.0 3,210,471 - Psest_3022 0.42 +1.6 3,210,522 + Psest_3022 0.59 -0.2 3,210,523 - Psest_3022 0.59 +0.0 3,210,743 + +1.4 3,210,744 - +1.2 3,210,744 - +0.4 3,210,744 - -0.3 3,210,750 + +0.2 3,210,750 + +0.8 3,210,750 + +1.4 3,210,750 + +0.4 3,210,750 + +0.9 3,210,750 + +0.9 3,210,750 + -0.1 3,210,751 - -0.4 3,210,751 - -0.9 3,210,751 - +0.2 3,210,751 - -1.9 3,210,751 - -1.2 3,210,751 - -0.2 3,210,751 - +1.2 3,210,751 - +0.2 3,210,751 - +3.1 3,210,751 - +0.6 3,210,751 - -0.6 3,210,751 - +3.6 3,210,751 - +0.5 3,210,751 - -0.5 3,210,751 - -0.1 3,210,751 - -1.8 3,210,751 - +0.1 3,210,751 - -0.5 3,210,751 - +1.8 3,210,803 + -2.6 3,210,804 - -0.1 3,210,804 - -0.3 3,210,863 + +0.1 3,210,864 - -1.1 3,210,864 - +0.2 3,210,864 - +0.9 3,210,864 - +0.4 3,210,864 - -0.3 3,210,879 - -0.1 3,210,940 - Psest_3024 0.12 -2.7 3,210,940 - Psest_3024 0.12 -1.2 3,211,163 + Psest_3024 0.32 +0.4 3,211,163 + Psest_3024 0.32 +0.4 3,211,163 + Psest_3024 0.32 +2.2 3,211,163 + Psest_3024 0.32 +0.8 3,211,164 - Psest_3024 0.32 +0.2 3,211,164 - Psest_3024 0.32 -0.3 3,211,164 - Psest_3024 0.32 -0.2 3,211,164 - Psest_3024 0.32 -0.9 3,211,164 - Psest_3024 0.32 +0.3 3,211,164 - Psest_3024 0.32 -0.9 3,211,312 + Psest_3024 0.45 -0.5 3,211,472 + Psest_3024 0.59 -0.1 3,211,473 - Psest_3024 0.59 -0.1
Or see this region's nucleotide sequence