Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2809 and Psest_2810 overlap by 4 nucleotides Psest_2810 and Psest_2811 overlap by 1 nucleotides Psest_2811 and Psest_2812 overlap by 4 nucleotides Psest_2812 and Psest_2813 are separated by 184 nucleotides Psest_2813 and Psest_2814 are separated by 38 nucleotides
Psest_2809: Psest_2809 - protein-L-isoaspartate(D-aspartate) O-methyltransferase, at 2,998,432 to 2,999,109
_2809
Psest_2810: Psest_2810 - 5'/3'-nucleotidase SurE, at 2,999,106 to 2,999,855
_2810
Psest_2811: Psest_2811 - Uncharacterized conserved protein, at 2,999,855 to 3,000,895
_2811
Psest_2812: Psest_2812 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 3,000,892 to 3,001,365
_2812
Psest_2813: Psest_2813 - Predicted transcriptional regulators, at 3,001,550 to 3,001,852
_2813
Psest_2814: Psest_2814 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 3,001,891 to 3,002,940
_2814
Position (kb)
2999
3000
3001 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2998.893 kb on + strand, within Psest_2809 at 2998.893 kb on + strand, within Psest_2809 at 2998.931 kb on + strand, within Psest_2809 at 2998.931 kb on + strand, within Psest_2809 at 2999.035 kb on + strand, within Psest_2809 at 2999.035 kb on + strand, within Psest_2809 at 2999.036 kb on - strand, within Psest_2809 at 2999.188 kb on - strand, within Psest_2810 at 2999.188 kb on - strand, within Psest_2810 at 2999.191 kb on + strand, within Psest_2810 at 2999.191 kb on + strand, within Psest_2810 at 2999.192 kb on - strand, within Psest_2810 at 2999.264 kb on - strand, within Psest_2810 at 2999.393 kb on + strand, within Psest_2810 at 2999.393 kb on + strand, within Psest_2810 at 2999.393 kb on + strand, within Psest_2810 at 2999.482 kb on - strand, within Psest_2810 at 2999.604 kb on - strand, within Psest_2810 at 2999.604 kb on - strand, within Psest_2810 at 2999.604 kb on - strand, within Psest_2810 at 2999.798 kb on - strand at 2999.821 kb on + strand at 2999.821 kb on + strand at 2999.822 kb on - strand at 2999.822 kb on - strand at 2999.822 kb on - strand at 2999.849 kb on + strand at 2999.850 kb on - strand at 3000.084 kb on - strand, within Psest_2811 at 3000.273 kb on - strand, within Psest_2811 at 3000.273 kb on - strand, within Psest_2811 at 3000.273 kb on - strand, within Psest_2811 at 3000.278 kb on - strand, within Psest_2811 at 3000.288 kb on + strand, within Psest_2811 at 3000.288 kb on + strand, within Psest_2811 at 3000.288 kb on + strand, within Psest_2811 at 3000.288 kb on + strand, within Psest_2811 at 3000.288 kb on + strand, within Psest_2811 at 3000.289 kb on - strand, within Psest_2811 at 3000.289 kb on - strand, within Psest_2811 at 3000.289 kb on - strand, within Psest_2811 at 3000.289 kb on - strand, within Psest_2811 at 3000.289 kb on - strand, within Psest_2811 at 3000.289 kb on - strand, within Psest_2811 at 3000.289 kb on - strand, within Psest_2811 at 3000.292 kb on + strand, within Psest_2811 at 3000.292 kb on + strand, within Psest_2811 at 3000.293 kb on - strand, within Psest_2811 at 3000.357 kb on + strand, within Psest_2811 at 3000.414 kb on + strand, within Psest_2811 at 3000.414 kb on + strand, within Psest_2811 at 3000.414 kb on + strand, within Psest_2811 at 3000.415 kb on - strand, within Psest_2811 at 3000.669 kb on + strand, within Psest_2811 at 3000.669 kb on + strand, within Psest_2811 at 3000.670 kb on - strand, within Psest_2811 at 3000.837 kb on - strand at 3001.366 kb on - strand at 3001.366 kb on - strand at 3001.366 kb on - strand at 3001.366 kb on - strand at 3001.366 kb on - strand at 3001.366 kb on - strand at 3001.366 kb on - strand at 3001.366 kb on - strand at 3001.366 kb on - strand at 3001.366 kb on - strand at 3001.366 kb on - strand at 3001.366 kb on - strand at 3001.366 kb on - strand at 3001.366 kb on - strand at 3001.403 kb on + strand at 3001.403 kb on + strand at 3001.404 kb on - strand at 3001.509 kb on - strand at 3001.511 kb on - strand at 3001.530 kb on + strand at 3001.530 kb on + strand at 3001.531 kb on - strand at 3001.531 kb on - strand at 3001.637 kb on + strand, within Psest_2813 at 3001.674 kb on + strand, within Psest_2813 at 3001.674 kb on + strand, within Psest_2813 at 3001.674 kb on + strand, within Psest_2813 at 3001.674 kb on + strand, within Psest_2813 at 3001.674 kb on + strand, within Psest_2813 at 3001.674 kb on + strand, within Psest_2813 at 3001.674 kb on + strand, within Psest_2813 at 3001.674 kb on + strand, within Psest_2813 at 3001.674 kb on + strand, within Psest_2813 at 3001.674 kb on + strand, within Psest_2813 at 3001.675 kb on - strand, within Psest_2813 at 3001.675 kb on - strand, within Psest_2813 at 3001.675 kb on - strand, within Psest_2813 at 3001.675 kb on - strand, within Psest_2813 at 3001.675 kb on - strand, within Psest_2813 at 3001.675 kb on - strand, within Psest_2813 at 3001.675 kb on - strand, within Psest_2813 at 3001.675 kb on - strand, within Psest_2813 at 3001.675 kb on - strand, within Psest_2813 at 3001.675 kb on - strand, within Psest_2813 at 3001.675 kb on - strand, within Psest_2813 at 3001.675 kb on - strand, within Psest_2813 at 3001.675 kb on - strand, within Psest_2813 at 3001.890 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 2,998,893 + Psest_2809 0.68 -0.4 2,998,893 + Psest_2809 0.68 +1.8 2,998,931 + Psest_2809 0.74 +1.5 2,998,931 + Psest_2809 0.74 +1.6 2,999,035 + Psest_2809 0.89 -1.3 2,999,035 + Psest_2809 0.89 -0.1 2,999,036 - Psest_2809 0.89 +0.1 2,999,188 - Psest_2810 0.11 +1.6 2,999,188 - Psest_2810 0.11 +1.7 2,999,191 + Psest_2810 0.11 +1.8 2,999,191 + Psest_2810 0.11 -1.7 2,999,192 - Psest_2810 0.11 -0.7 2,999,264 - Psest_2810 0.21 +1.7 2,999,393 + Psest_2810 0.38 +1.0 2,999,393 + Psest_2810 0.38 +0.7 2,999,393 + Psest_2810 0.38 +1.3 2,999,482 - Psest_2810 0.50 +2.9 2,999,604 - Psest_2810 0.66 +1.2 2,999,604 - Psest_2810 0.66 +2.5 2,999,604 - Psest_2810 0.66 +0.6 2,999,798 - +0.7 2,999,821 + +1.3 2,999,821 + +1.3 2,999,822 - +3.0 2,999,822 - +1.6 2,999,822 - +1.9 2,999,849 + +2.6 2,999,850 - -0.9 3,000,084 - Psest_2811 0.22 +0.0 3,000,273 - Psest_2811 0.40 +1.6 3,000,273 - Psest_2811 0.40 +1.7 3,000,273 - Psest_2811 0.40 +0.3 3,000,278 - Psest_2811 0.41 +1.3 3,000,288 + Psest_2811 0.42 +0.9 3,000,288 + Psest_2811 0.42 +0.0 3,000,288 + Psest_2811 0.42 +0.8 3,000,288 + Psest_2811 0.42 +1.3 3,000,288 + Psest_2811 0.42 +1.0 3,000,289 - Psest_2811 0.42 -0.4 3,000,289 - Psest_2811 0.42 +0.9 3,000,289 - Psest_2811 0.42 +0.6 3,000,289 - Psest_2811 0.42 +0.9 3,000,289 - Psest_2811 0.42 +1.8 3,000,289 - Psest_2811 0.42 +0.0 3,000,289 - Psest_2811 0.42 +1.0 3,000,292 + Psest_2811 0.42 -0.3 3,000,292 + Psest_2811 0.42 +2.6 3,000,293 - Psest_2811 0.42 +0.3 3,000,357 + Psest_2811 0.48 +1.4 3,000,414 + Psest_2811 0.54 +0.7 3,000,414 + Psest_2811 0.54 +0.2 3,000,414 + Psest_2811 0.54 +1.4 3,000,415 - Psest_2811 0.54 +1.2 3,000,669 + Psest_2811 0.78 -0.0 3,000,669 + Psest_2811 0.78 +2.7 3,000,670 - Psest_2811 0.78 +0.3 3,000,837 - +0.3 3,001,366 - -0.7 3,001,366 - +1.3 3,001,366 - -1.7 3,001,366 - +2.5 3,001,366 - -1.5 3,001,366 - +2.0 3,001,366 - +0.6 3,001,366 - -0.9 3,001,366 - +0.3 3,001,366 - -0.3 3,001,366 - +1.0 3,001,366 - -0.2 3,001,366 - -0.0 3,001,366 - -1.0 3,001,403 + +2.6 3,001,403 + +0.2 3,001,404 - +1.5 3,001,509 - +0.3 3,001,511 - +0.3 3,001,530 + -0.3 3,001,530 + +1.2 3,001,531 - -1.3 3,001,531 - +3.0 3,001,637 + Psest_2813 0.29 +0.0 3,001,674 + Psest_2813 0.41 +0.1 3,001,674 + Psest_2813 0.41 -0.7 3,001,674 + Psest_2813 0.41 -0.3 3,001,674 + Psest_2813 0.41 +2.6 3,001,674 + Psest_2813 0.41 +0.6 3,001,674 + Psest_2813 0.41 +1.2 3,001,674 + Psest_2813 0.41 +0.2 3,001,674 + Psest_2813 0.41 -0.7 3,001,674 + Psest_2813 0.41 +0.1 3,001,674 + Psest_2813 0.41 -0.0 3,001,675 - Psest_2813 0.41 -0.6 3,001,675 - Psest_2813 0.41 -0.6 3,001,675 - Psest_2813 0.41 -1.5 3,001,675 - Psest_2813 0.41 -0.8 3,001,675 - Psest_2813 0.41 -1.2 3,001,675 - Psest_2813 0.41 -0.8 3,001,675 - Psest_2813 0.41 -2.3 3,001,675 - Psest_2813 0.41 -0.5 3,001,675 - Psest_2813 0.41 -0.6 3,001,675 - Psest_2813 0.41 +1.1 3,001,675 - Psest_2813 0.41 +0.1 3,001,675 - Psest_2813 0.41 -1.0 3,001,675 - Psest_2813 0.41 -0.8 3,001,890 + -1.1
Or see this region's nucleotide sequence