Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2236 and Psest_2237 are separated by 89 nucleotides Psest_2237 and Psest_2238 are separated by 260 nucleotides Psest_2238 and Psest_2239 overlap by 1 nucleotides Psest_2239 and Psest_2240 are separated by 136 nucleotides
Psest_2236: Psest_2236 - Rad3-related DNA helicases, at 2,388,243 to 2,390,522
_2236
Psest_2237: Psest_2237 - tRNA_Leu_TAA, at 2,390,612 to 2,390,698
_2237
Psest_2238: Psest_2238 - hypothetical protein, at 2,390,959 to 2,391,366
_2238
Psest_2239: Psest_2239 - RES domain., at 2,391,366 to 2,392,052
_2239
Psest_2240: Psest_2240 - hypothetical protein, at 2,392,189 to 2,392,368
_2240
Position (kb)
2390
2391
2392 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2390.028 kb on + strand, within Psest_2236 at 2390.028 kb on + strand, within Psest_2236 at 2390.028 kb on + strand, within Psest_2236 at 2390.028 kb on + strand, within Psest_2236 at 2390.028 kb on + strand, within Psest_2236 at 2390.029 kb on - strand, within Psest_2236 at 2390.029 kb on - strand, within Psest_2236 at 2390.029 kb on - strand, within Psest_2236 at 2390.029 kb on - strand, within Psest_2236 at 2390.029 kb on - strand, within Psest_2236 at 2390.029 kb on - strand, within Psest_2236 at 2390.055 kb on + strand, within Psest_2236 at 2390.198 kb on - strand, within Psest_2236 at 2390.326 kb on + strand at 2390.326 kb on + strand at 2390.352 kb on + strand at 2390.352 kb on + strand at 2390.353 kb on - strand at 2390.353 kb on - strand at 2390.353 kb on - strand at 2390.358 kb on + strand at 2390.358 kb on + strand at 2390.358 kb on + strand at 2390.358 kb on + strand at 2390.358 kb on + strand at 2390.359 kb on - strand at 2390.359 kb on - strand at 2390.359 kb on - strand at 2390.359 kb on - strand at 2390.359 kb on - strand at 2390.359 kb on - strand at 2390.359 kb on - strand at 2390.359 kb on - strand at 2390.359 kb on - strand at 2390.359 kb on - strand at 2390.359 kb on - strand at 2390.359 kb on - strand at 2390.741 kb on + strand at 2390.762 kb on + strand at 2390.762 kb on + strand at 2390.763 kb on - strand at 2390.763 kb on - strand at 2390.844 kb on + strand at 2390.845 kb on - strand at 2390.888 kb on + strand at 2390.889 kb on - strand at 2390.889 kb on - strand at 2390.958 kb on + strand at 2391.042 kb on + strand, within Psest_2238 at 2391.042 kb on + strand, within Psest_2238 at 2391.042 kb on + strand, within Psest_2238 at 2391.043 kb on - strand, within Psest_2238 at 2391.058 kb on - strand, within Psest_2238 at 2391.184 kb on - strand, within Psest_2238 at 2391.225 kb on + strand, within Psest_2238 at 2391.226 kb on - strand, within Psest_2238 at 2391.664 kb on - strand, within Psest_2239 at 2391.664 kb on - strand, within Psest_2239 at 2391.720 kb on + strand, within Psest_2239 at 2391.720 kb on + strand, within Psest_2239 at 2391.837 kb on + strand, within Psest_2239 at 2391.852 kb on + strand, within Psest_2239 at 2392.106 kb on - strand at 2392.106 kb on - strand at 2392.248 kb on + strand, within Psest_2240 at 2392.249 kb on - strand, within Psest_2240 at 2392.249 kb on - strand, within Psest_2240 at 2392.366 kb on + strand at 2392.366 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 2,390,028 + Psest_2236 0.78 -0.3 2,390,028 + Psest_2236 0.78 +0.3 2,390,028 + Psest_2236 0.78 -2.0 2,390,028 + Psest_2236 0.78 +0.1 2,390,028 + Psest_2236 0.78 -0.1 2,390,029 - Psest_2236 0.78 +1.4 2,390,029 - Psest_2236 0.78 +2.2 2,390,029 - Psest_2236 0.78 -2.4 2,390,029 - Psest_2236 0.78 +0.4 2,390,029 - Psest_2236 0.78 +3.1 2,390,029 - Psest_2236 0.78 -1.9 2,390,055 + Psest_2236 0.79 -0.6 2,390,198 - Psest_2236 0.86 +0.3 2,390,326 + +1.0 2,390,326 + -0.9 2,390,352 + +0.3 2,390,352 + -0.7 2,390,353 - +0.0 2,390,353 - -1.3 2,390,353 - +1.5 2,390,358 + -1.3 2,390,358 + +1.7 2,390,358 + -1.1 2,390,358 + +0.5 2,390,358 + -0.3 2,390,359 - -0.4 2,390,359 - +0.2 2,390,359 - +0.2 2,390,359 - -0.2 2,390,359 - +0.8 2,390,359 - +0.1 2,390,359 - +0.7 2,390,359 - -1.7 2,390,359 - +0.3 2,390,359 - +0.1 2,390,359 - +0.1 2,390,359 - -1.9 2,390,741 + +0.0 2,390,762 + +0.5 2,390,762 + -1.6 2,390,763 - -0.7 2,390,763 - -0.7 2,390,844 + +0.5 2,390,845 - +0.5 2,390,888 + +0.2 2,390,889 - -0.2 2,390,889 - -0.1 2,390,958 + +0.1 2,391,042 + Psest_2238 0.20 +1.1 2,391,042 + Psest_2238 0.20 +2.4 2,391,042 + Psest_2238 0.20 +0.5 2,391,043 - Psest_2238 0.21 +2.3 2,391,058 - Psest_2238 0.24 +1.6 2,391,184 - Psest_2238 0.55 -0.8 2,391,225 + Psest_2238 0.65 -0.1 2,391,226 - Psest_2238 0.65 +2.3 2,391,664 - Psest_2239 0.43 -0.9 2,391,664 - Psest_2239 0.43 -0.9 2,391,720 + Psest_2239 0.52 -0.4 2,391,720 + Psest_2239 0.52 +0.4 2,391,837 + Psest_2239 0.69 +1.9 2,391,852 + Psest_2239 0.71 -1.2 2,392,106 - -0.6 2,392,106 - +0.4 2,392,248 + Psest_2240 0.33 -1.5 2,392,249 - Psest_2240 0.33 +2.3 2,392,249 - Psest_2240 0.33 -0.7 2,392,366 + +0.3 2,392,366 + -0.9
Or see this region's nucleotide sequence