Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1999 and Psest_2000 are separated by 68 nucleotides Psest_2000 and Psest_2001 are separated by 80 nucleotides Psest_2001 and Psest_2002 overlap by 1 nucleotides
Psest_1999: Psest_1999 - pseudouridine synthase, at 2,116,169 to 2,117,302
_1999
Psest_2000: Psest_2000 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 2,117,371 to 2,118,111
_2000
Psest_2001: Psest_2001 - 2-phosphoglycolate phosphatase, prokaryotic, at 2,118,192 to 2,118,863
_2001
Psest_2002: Psest_2002 - ubiquinone biosynthesis O-methyltransferase, at 2,118,863 to 2,119,561
_2002
Position (kb)
2117
2118
2119 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2116.412 kb on + strand, within Psest_1999 at 2116.616 kb on + strand, within Psest_1999 at 2117.046 kb on + strand, within Psest_1999 at 2117.287 kb on + strand at 2117.287 kb on + strand at 2117.303 kb on + strand at 2117.303 kb on + strand at 2117.303 kb on + strand at 2117.304 kb on - strand at 2117.361 kb on + strand at 2117.426 kb on + strand at 2117.426 kb on + strand at 2117.426 kb on + strand at 2117.427 kb on - strand at 2117.427 kb on - strand at 2117.427 kb on - strand at 2117.427 kb on - strand at 2117.432 kb on + strand at 2117.432 kb on + strand at 2117.432 kb on + strand at 2117.432 kb on + strand at 2117.433 kb on - strand at 2117.433 kb on - strand at 2117.433 kb on - strand at 2117.433 kb on - strand at 2117.487 kb on - strand, within Psest_2000 at 2117.487 kb on - strand, within Psest_2000 at 2117.502 kb on + strand, within Psest_2000 at 2117.573 kb on - strand, within Psest_2000 at 2117.575 kb on + strand, within Psest_2000 at 2117.575 kb on + strand, within Psest_2000 at 2117.628 kb on - strand, within Psest_2000 at 2117.628 kb on - strand, within Psest_2000 at 2117.791 kb on - strand, within Psest_2000 at 2117.873 kb on + strand, within Psest_2000 at 2117.952 kb on - strand, within Psest_2000 at 2117.989 kb on + strand, within Psest_2000 at 2117.990 kb on - strand, within Psest_2000 at 2117.992 kb on + strand, within Psest_2000 at 2117.992 kb on + strand, within Psest_2000 at 2118.102 kb on - strand at 2118.124 kb on - strand at 2118.124 kb on - strand at 2118.135 kb on + strand at 2118.136 kb on - strand at 2118.136 kb on - strand at 2118.138 kb on + strand at 2118.188 kb on + strand at 2118.295 kb on + strand, within Psest_2001 at 2118.295 kb on + strand, within Psest_2001 at 2118.295 kb on + strand, within Psest_2001 at 2118.347 kb on + strand, within Psest_2001 at 2118.437 kb on + strand, within Psest_2001 at 2118.590 kb on + strand, within Psest_2001 at 2118.874 kb on + strand at 2118.874 kb on + strand at 2118.874 kb on + strand at 2118.874 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 2,116,412 + Psest_1999 0.21 -1.0 2,116,616 + Psest_1999 0.39 -1.5 2,117,046 + Psest_1999 0.77 +3.0 2,117,287 + -1.4 2,117,287 + -0.4 2,117,303 + -0.6 2,117,303 + +1.3 2,117,303 + +0.0 2,117,304 - +0.5 2,117,361 + +1.2 2,117,426 + +1.2 2,117,426 + +1.2 2,117,426 + -1.6 2,117,427 - +1.7 2,117,427 - +0.7 2,117,427 - +3.6 2,117,427 - -0.7 2,117,432 + +1.3 2,117,432 + +1.8 2,117,432 + +2.0 2,117,432 + +3.1 2,117,433 - +2.4 2,117,433 - +2.7 2,117,433 - +0.7 2,117,433 - +0.8 2,117,487 - Psest_2000 0.16 +2.3 2,117,487 - Psest_2000 0.16 +0.6 2,117,502 + Psest_2000 0.18 +0.8 2,117,573 - Psest_2000 0.27 +2.2 2,117,575 + Psest_2000 0.28 -1.5 2,117,575 + Psest_2000 0.28 +0.9 2,117,628 - Psest_2000 0.35 +0.8 2,117,628 - Psest_2000 0.35 +1.1 2,117,791 - Psest_2000 0.57 +0.9 2,117,873 + Psest_2000 0.68 -1.4 2,117,952 - Psest_2000 0.78 +1.5 2,117,989 + Psest_2000 0.83 -0.0 2,117,990 - Psest_2000 0.84 +3.1 2,117,992 + Psest_2000 0.84 +1.2 2,117,992 + Psest_2000 0.84 +1.5 2,118,102 - +1.8 2,118,124 - +0.8 2,118,124 - +0.9 2,118,135 + +1.0 2,118,136 - -0.2 2,118,136 - -2.6 2,118,138 + +3.5 2,118,188 + +0.1 2,118,295 + Psest_2001 0.15 +0.3 2,118,295 + Psest_2001 0.15 +0.3 2,118,295 + Psest_2001 0.15 +0.3 2,118,347 + Psest_2001 0.23 -1.0 2,118,437 + Psest_2001 0.36 +1.3 2,118,590 + Psest_2001 0.59 -1.0 2,118,874 + +0.7 2,118,874 + +0.3 2,118,874 + -0.7 2,118,874 + -0.7
Or see this region's nucleotide sequence