Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1949 and Psest_1950 are separated by 39 nucleotides Psest_1950 and Psest_1951 are separated by 272 nucleotides Psest_1951 and Psest_1952 overlap by 17 nucleotides
Psest_1949: Psest_1949 - Predicted N6-adenine-specific DNA methylase, at 2,065,390 to 2,067,570
_1949
Psest_1950: Psest_1950 - D-alanyl-D-alanine carboxypeptidase, serine-type, PBP4 family, at 2,067,610 to 2,069,067
_1950
Psest_1951: Psest_1951 - Uncharacterized protein conserved in bacteria, at 2,069,340 to 2,069,684
_1951
Psest_1952: Psest_1952 - hypothetical protein, at 2,069,668 to 2,070,759
_1952
Position (kb)
2067
2068
2069
2070 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2066.732 kb on - strand, within Psest_1949 at 2066.732 kb on - strand, within Psest_1949 at 2066.800 kb on + strand, within Psest_1949 at 2067.000 kb on - strand, within Psest_1949 at 2067.000 kb on - strand, within Psest_1949 at 2067.020 kb on - strand, within Psest_1949 at 2067.112 kb on + strand, within Psest_1949 at 2067.187 kb on + strand, within Psest_1949 at 2067.187 kb on + strand, within Psest_1949 at 2067.187 kb on + strand, within Psest_1949 at 2067.207 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.261 kb on - strand, within Psest_1949 at 2067.424 kb on + strand at 2067.424 kb on + strand at 2067.424 kb on + strand at 2067.424 kb on + strand at 2067.424 kb on + strand at 2067.424 kb on + strand at 2067.644 kb on + strand at 2067.645 kb on - strand at 2067.819 kb on + strand, within Psest_1950 at 2067.819 kb on + strand, within Psest_1950 at 2067.819 kb on + strand, within Psest_1950 at 2067.819 kb on + strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.873 kb on + strand, within Psest_1950 at 2067.874 kb on - strand, within Psest_1950 at 2067.874 kb on - strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.272 kb on - strand, within Psest_1950 at 2068.298 kb on + strand, within Psest_1950 at 2068.298 kb on + strand, within Psest_1950 at 2068.299 kb on - strand, within Psest_1950 at 2068.299 kb on - strand, within Psest_1950 at 2068.343 kb on - strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.377 kb on - strand, within Psest_1950 at 2068.377 kb on - strand, within Psest_1950 at 2068.377 kb on - strand, within Psest_1950 at 2068.377 kb on - strand, within Psest_1950 at 2068.377 kb on - strand, within Psest_1950 at 2068.381 kb on + strand, within Psest_1950 at 2068.381 kb on + strand, within Psest_1950 at 2068.381 kb on + strand, within Psest_1950 at 2068.381 kb on + strand, within Psest_1950 at 2068.382 kb on - strand, within Psest_1950 at 2068.382 kb on - strand, within Psest_1950 at 2068.382 kb on - strand, within Psest_1950 at 2068.382 kb on - strand, within Psest_1950 at 2068.423 kb on + strand, within Psest_1950 at 2068.727 kb on + strand, within Psest_1950 at 2068.728 kb on - strand, within Psest_1950 at 2068.772 kb on + strand, within Psest_1950 at 2068.772 kb on + strand, within Psest_1950 at 2068.773 kb on - strand, within Psest_1950 at 2068.773 kb on - strand, within Psest_1950 at 2068.773 kb on - strand, within Psest_1950 at 2068.804 kb on + strand, within Psest_1950 at 2068.804 kb on + strand, within Psest_1950 at 2068.805 kb on - strand, within Psest_1950 at 2068.805 kb on - strand, within Psest_1950 at 2068.805 kb on - strand, within Psest_1950 at 2068.823 kb on + strand, within Psest_1950 at 2068.823 kb on + strand, within Psest_1950 at 2068.824 kb on - strand, within Psest_1950 at 2068.824 kb on - strand, within Psest_1950 at 2068.824 kb on - strand, within Psest_1950 at 2068.824 kb on - strand, within Psest_1950 at 2068.824 kb on - strand, within Psest_1950 at 2068.991 kb on - strand at 2068.991 kb on - strand at 2069.043 kb on + strand at 2069.043 kb on + strand at 2069.043 kb on + strand at 2069.044 kb on - strand at 2069.069 kb on + strand at 2069.069 kb on + strand at 2069.069 kb on + strand at 2069.069 kb on + strand at 2069.070 kb on - strand at 2069.070 kb on - strand at 2069.201 kb on + strand at 2069.202 kb on - strand at 2069.202 kb on - strand at 2069.210 kb on + strand at 2069.210 kb on + strand at 2069.210 kb on + strand at 2069.212 kb on + strand at 2069.212 kb on + strand at 2069.213 kb on - strand at 2069.232 kb on + strand at 2069.232 kb on + strand at 2069.232 kb on + strand at 2069.233 kb on - strand at 2069.234 kb on + strand at 2069.234 kb on + strand at 2069.234 kb on + strand at 2069.234 kb on + strand at 2069.234 kb on + strand at 2069.234 kb on + strand at 2069.234 kb on + strand at 2069.234 kb on + strand at 2069.234 kb on + strand at 2069.235 kb on - strand at 2069.235 kb on - strand at 2069.235 kb on - strand at 2069.235 kb on - strand at 2069.235 kb on - strand at 2069.235 kb on - strand at 2069.235 kb on - strand at 2069.322 kb on - strand at 2069.509 kb on - strand, within Psest_1951 at 2069.509 kb on - strand, within Psest_1951 at 2069.622 kb on + strand, within Psest_1951 at 2069.682 kb on + strand at 2069.683 kb on - strand at 2069.990 kb on + strand, within Psest_1952 at 2069.991 kb on - strand, within Psest_1952 at 2069.991 kb on - strand, within Psest_1952 at 2069.991 kb on - strand, within Psest_1952
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 2,066,732 - Psest_1949 0.62 +0.7 2,066,732 - Psest_1949 0.62 -1.4 2,066,800 + Psest_1949 0.65 +0.5 2,067,000 - Psest_1949 0.74 -0.7 2,067,000 - Psest_1949 0.74 +1.8 2,067,020 - Psest_1949 0.75 -0.2 2,067,112 + Psest_1949 0.79 -2.5 2,067,187 + Psest_1949 0.82 +0.6 2,067,187 + Psest_1949 0.82 -1.0 2,067,187 + Psest_1949 0.82 -0.5 2,067,207 + Psest_1949 0.83 +0.4 2,067,260 + Psest_1949 0.86 +1.0 2,067,260 + Psest_1949 0.86 +0.0 2,067,260 + Psest_1949 0.86 -0.6 2,067,260 + Psest_1949 0.86 +0.9 2,067,260 + Psest_1949 0.86 +0.4 2,067,260 + Psest_1949 0.86 -0.1 2,067,260 + Psest_1949 0.86 -1.2 2,067,260 + Psest_1949 0.86 -0.4 2,067,261 - Psest_1949 0.86 -1.7 2,067,424 + -0.7 2,067,424 + -1.9 2,067,424 + +0.9 2,067,424 + +0.3 2,067,424 + +1.1 2,067,424 + -0.3 2,067,644 + -0.5 2,067,645 - -0.4 2,067,819 + Psest_1950 0.14 -0.7 2,067,819 + Psest_1950 0.14 +0.5 2,067,819 + Psest_1950 0.14 -1.0 2,067,819 + Psest_1950 0.14 -0.5 2,067,820 - Psest_1950 0.14 -1.3 2,067,820 - Psest_1950 0.14 -0.2 2,067,820 - Psest_1950 0.14 -1.2 2,067,820 - Psest_1950 0.14 -0.2 2,067,820 - Psest_1950 0.14 -1.7 2,067,820 - Psest_1950 0.14 +0.4 2,067,820 - Psest_1950 0.14 -1.3 2,067,820 - Psest_1950 0.14 -0.6 2,067,820 - Psest_1950 0.14 -0.7 2,067,820 - Psest_1950 0.14 -1.5 2,067,873 + Psest_1950 0.18 +0.1 2,067,874 - Psest_1950 0.18 -1.7 2,067,874 - Psest_1950 0.18 -0.7 2,068,121 + Psest_1950 0.35 -2.5 2,068,121 + Psest_1950 0.35 -0.6 2,068,121 + Psest_1950 0.35 -0.2 2,068,121 + Psest_1950 0.35 -0.2 2,068,121 + Psest_1950 0.35 -1.4 2,068,121 + Psest_1950 0.35 -2.3 2,068,121 + Psest_1950 0.35 -1.1 2,068,121 + Psest_1950 0.35 -0.5 2,068,122 - Psest_1950 0.35 -1.6 2,068,122 - Psest_1950 0.35 -0.9 2,068,122 - Psest_1950 0.35 -1.5 2,068,122 - Psest_1950 0.35 -1.2 2,068,122 - Psest_1950 0.35 -2.4 2,068,122 - Psest_1950 0.35 -0.4 2,068,122 - Psest_1950 0.35 -1.3 2,068,272 - Psest_1950 0.45 -2.0 2,068,298 + Psest_1950 0.47 -3.2 2,068,298 + Psest_1950 0.47 -0.1 2,068,299 - Psest_1950 0.47 -2.2 2,068,299 - Psest_1950 0.47 -0.9 2,068,343 - Psest_1950 0.50 -1.2 2,068,376 + Psest_1950 0.53 -1.1 2,068,376 + Psest_1950 0.53 +0.3 2,068,376 + Psest_1950 0.53 -1.0 2,068,376 + Psest_1950 0.53 -2.0 2,068,376 + Psest_1950 0.53 -2.6 2,068,376 + Psest_1950 0.53 +0.3 2,068,377 - Psest_1950 0.53 -0.7 2,068,377 - Psest_1950 0.53 -2.5 2,068,377 - Psest_1950 0.53 -1.8 2,068,377 - Psest_1950 0.53 -0.1 2,068,377 - Psest_1950 0.53 -1.9 2,068,381 + Psest_1950 0.53 -1.6 2,068,381 + Psest_1950 0.53 -0.3 2,068,381 + Psest_1950 0.53 -0.6 2,068,381 + Psest_1950 0.53 -0.0 2,068,382 - Psest_1950 0.53 -1.4 2,068,382 - Psest_1950 0.53 -0.9 2,068,382 - Psest_1950 0.53 -0.8 2,068,382 - Psest_1950 0.53 -0.3 2,068,423 + Psest_1950 0.56 -1.3 2,068,727 + Psest_1950 0.77 -1.1 2,068,728 - Psest_1950 0.77 -2.1 2,068,772 + Psest_1950 0.80 -1.3 2,068,772 + Psest_1950 0.80 -4.0 2,068,773 - Psest_1950 0.80 -0.4 2,068,773 - Psest_1950 0.80 -0.6 2,068,773 - Psest_1950 0.80 -0.4 2,068,804 + Psest_1950 0.82 -0.3 2,068,804 + Psest_1950 0.82 -1.0 2,068,805 - Psest_1950 0.82 -0.5 2,068,805 - Psest_1950 0.82 -1.4 2,068,805 - Psest_1950 0.82 -0.8 2,068,823 + Psest_1950 0.83 -0.9 2,068,823 + Psest_1950 0.83 -2.1 2,068,824 - Psest_1950 0.83 -1.0 2,068,824 - Psest_1950 0.83 -0.9 2,068,824 - Psest_1950 0.83 -1.6 2,068,824 - Psest_1950 0.83 +0.7 2,068,824 - Psest_1950 0.83 +0.3 2,068,991 - +0.5 2,068,991 - -0.9 2,069,043 + -1.3 2,069,043 + +0.2 2,069,043 + -2.4 2,069,044 - -2.9 2,069,069 + -1.0 2,069,069 + -1.0 2,069,069 + -0.7 2,069,069 + +0.6 2,069,070 - -0.3 2,069,070 - +0.3 2,069,201 + -0.5 2,069,202 - +1.4 2,069,202 - -2.6 2,069,210 + +1.0 2,069,210 + +0.4 2,069,210 + -0.1 2,069,212 + -1.7 2,069,212 + -1.0 2,069,213 - +0.3 2,069,232 + -1.5 2,069,232 + -0.8 2,069,232 + -0.4 2,069,233 - -0.7 2,069,234 + -0.4 2,069,234 + +1.7 2,069,234 + -0.2 2,069,234 + +1.1 2,069,234 + -0.7 2,069,234 + -1.1 2,069,234 + -0.4 2,069,234 + -1.7 2,069,234 + +0.2 2,069,235 - +0.7 2,069,235 - +1.0 2,069,235 - -0.7 2,069,235 - -1.7 2,069,235 - +0.3 2,069,235 - +1.8 2,069,235 - -0.0 2,069,322 - -0.5 2,069,509 - Psest_1951 0.49 -0.9 2,069,509 - Psest_1951 0.49 +0.8 2,069,622 + Psest_1951 0.82 +0.2 2,069,682 + +1.5 2,069,683 - +0.3 2,069,990 + Psest_1952 0.29 +0.0 2,069,991 - Psest_1952 0.30 -0.5 2,069,991 - Psest_1952 0.30 +2.6 2,069,991 - Psest_1952 0.30 -2.0
Or see this region's nucleotide sequence