Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1904 and Psest_1905 are separated by 116 nucleotides Psest_1905 and Psest_1907 are separated by 284 nucleotides Psest_1907 and Psest_1908 are separated by 3 nucleotides Psest_1908 and Psest_1909 are separated by 3 nucleotides Psest_1909 and Psest_1910 overlap by 4 nucleotides
Psest_1904: Psest_1904 - Arabinose efflux permease, at 2,014,700 to 2,015,851
_1904
Psest_1905: Psest_1905 - Uncharacterized conserved protein, contains double-stranded beta-helix domain, at 2,015,968 to 2,016,513
_1905
Psest_1907: Psest_1907 - hypothetical protein, at 2,016,798 to 2,017,007
_1907
Psest_1908: Psest_1908 - hypothetical protein, at 2,017,011 to 2,017,205
_1908
Psest_1909: Psest_1909 - Phosphohistidine phosphatase SixA, at 2,017,209 to 2,017,766
_1909
Psest_1910: Psest_1910 - 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase, at 2,017,763 to 2,018,647
_1910
Position (kb)
2016
2017
2018 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2015.816 kb on - strand at 2016.334 kb on + strand, within Psest_1905 at 2016.341 kb on + strand, within Psest_1905 at 2016.341 kb on + strand, within Psest_1905 at 2016.439 kb on - strand, within Psest_1905 at 2016.467 kb on - strand at 2016.565 kb on - strand at 2016.565 kb on - strand at 2016.565 kb on - strand at 2016.594 kb on + strand at 2016.594 kb on + strand at 2016.594 kb on + strand at 2016.594 kb on + strand at 2016.595 kb on - strand at 2016.595 kb on - strand at 2016.595 kb on - strand at 2016.683 kb on + strand at 2016.684 kb on - strand at 2016.764 kb on + strand at 2016.764 kb on + strand at 2016.764 kb on + strand at 2016.764 kb on + strand at 2016.765 kb on - strand at 2016.765 kb on - strand at 2016.769 kb on + strand at 2016.769 kb on + strand at 2016.769 kb on + strand at 2016.769 kb on + strand at 2016.770 kb on - strand at 2016.770 kb on - strand at 2016.770 kb on - strand at 2016.770 kb on - strand at 2016.770 kb on - strand at 2016.770 kb on - strand at 2016.773 kb on + strand at 2016.774 kb on - strand at 2016.848 kb on + strand, within Psest_1907 at 2017.129 kb on + strand, within Psest_1908 at 2017.203 kb on + strand at 2017.203 kb on + strand at 2017.312 kb on + strand, within Psest_1909 at 2017.313 kb on - strand, within Psest_1909 at 2017.313 kb on - strand, within Psest_1909 at 2017.313 kb on - strand, within Psest_1909 at 2017.466 kb on + strand, within Psest_1909 at 2017.467 kb on - strand, within Psest_1909 at 2017.467 kb on - strand, within Psest_1909 at 2017.467 kb on - strand, within Psest_1909 at 2017.741 kb on + strand at 2017.788 kb on + strand at 2017.789 kb on - strand at 2017.789 kb on - strand at 2017.959 kb on + strand, within Psest_1910 at 2017.960 kb on - strand, within Psest_1910 at 2017.960 kb on - strand, within Psest_1910
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 2,015,816 - -0.0 2,016,334 + Psest_1905 0.67 -0.0 2,016,341 + Psest_1905 0.68 -0.7 2,016,341 + Psest_1905 0.68 +1.3 2,016,439 - Psest_1905 0.86 -0.9 2,016,467 - -0.6 2,016,565 - +0.3 2,016,565 - +1.8 2,016,565 - +2.1 2,016,594 + -0.6 2,016,594 + +0.1 2,016,594 + +0.1 2,016,594 + -2.4 2,016,595 - -0.0 2,016,595 - -1.1 2,016,595 - +0.3 2,016,683 + -1.1 2,016,684 - +2.4 2,016,764 + -1.1 2,016,764 + -0.6 2,016,764 + +0.3 2,016,764 + +1.4 2,016,765 - -0.3 2,016,765 - +0.7 2,016,769 + -0.8 2,016,769 + +1.9 2,016,769 + -0.9 2,016,769 + +0.5 2,016,770 - +0.0 2,016,770 - +0.0 2,016,770 - -2.0 2,016,770 - +1.9 2,016,770 - -1.2 2,016,770 - +0.6 2,016,773 + +0.0 2,016,774 - -0.8 2,016,848 + Psest_1907 0.24 +1.1 2,017,129 + Psest_1908 0.61 +0.1 2,017,203 + -1.3 2,017,203 + +0.9 2,017,312 + Psest_1909 0.18 -0.2 2,017,313 - Psest_1909 0.19 +0.8 2,017,313 - Psest_1909 0.19 +1.9 2,017,313 - Psest_1909 0.19 +0.2 2,017,466 + Psest_1909 0.46 -0.6 2,017,467 - Psest_1909 0.46 -0.1 2,017,467 - Psest_1909 0.46 +2.1 2,017,467 - Psest_1909 0.46 +1.1 2,017,741 + -1.7 2,017,788 + +0.2 2,017,789 - +3.1 2,017,789 - -1.2 2,017,959 + Psest_1910 0.22 -1.6 2,017,960 - Psest_1910 0.22 -0.5 2,017,960 - Psest_1910 0.22 +3.5
Or see this region's nucleotide sequence