Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1719 and Psest_1720 overlap by 8 nucleotides Psest_1720 and Psest_1721 are separated by 19 nucleotides Psest_1721 and Psest_1722 overlap by 11 nucleotides
Psest_1719: Psest_1719 - flagellar basal-body M-ring protein/flagellar hook-basal body protein (fliF), at 1,825,053 to 1,826,837
_1719
Psest_1720: Psest_1720 - flagellar motor switch protein FliG, at 1,826,830 to 1,827,846
_1720
Psest_1721: Psest_1721 - Flagellar biosynthesis/type III secretory pathway protein, at 1,827,866 to 1,828,642
_1721
Psest_1722: Psest_1722 - flagellar protein export ATPase FliI, at 1,828,632 to 1,829,990
_1722
Position (kb)
1826
1827
1828 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1825.833 kb on + strand, within Psest_1719 at 1826.223 kb on + strand, within Psest_1719 at 1826.224 kb on - strand, within Psest_1719 at 1826.224 kb on - strand, within Psest_1719 at 1826.247 kb on + strand, within Psest_1719 at 1826.247 kb on + strand, within Psest_1719 at 1826.248 kb on - strand, within Psest_1719 at 1826.248 kb on - strand, within Psest_1719 at 1826.248 kb on - strand, within Psest_1719 at 1826.248 kb on - strand, within Psest_1719 at 1826.248 kb on - strand, within Psest_1719 at 1826.248 kb on - strand, within Psest_1719 at 1826.248 kb on - strand, within Psest_1719 at 1826.249 kb on + strand, within Psest_1719 at 1826.250 kb on - strand, within Psest_1719 at 1826.250 kb on - strand, within Psest_1719 at 1827.073 kb on + strand, within Psest_1720 at 1827.073 kb on + strand, within Psest_1720 at 1827.073 kb on + strand, within Psest_1720 at 1827.073 kb on + strand, within Psest_1720 at 1827.074 kb on - strand, within Psest_1720 at 1827.136 kb on - strand, within Psest_1720 at 1827.153 kb on + strand, within Psest_1720 at 1827.251 kb on - strand, within Psest_1720 at 1827.425 kb on + strand, within Psest_1720 at 1827.425 kb on + strand, within Psest_1720 at 1827.425 kb on + strand, within Psest_1720 at 1827.426 kb on - strand, within Psest_1720 at 1827.460 kb on + strand, within Psest_1720 at 1827.460 kb on + strand, within Psest_1720 at 1827.460 kb on + strand, within Psest_1720 at 1827.460 kb on + strand, within Psest_1720 at 1827.460 kb on + strand, within Psest_1720 at 1827.460 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.815 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.845 kb on - strand at 1827.845 kb on - strand at 1827.852 kb on + strand at 1827.861 kb on + strand at 1828.088 kb on + strand, within Psest_1721 at 1828.088 kb on + strand, within Psest_1721 at 1828.089 kb on - strand, within Psest_1721 at 1828.089 kb on - strand, within Psest_1721 at 1828.140 kb on + strand, within Psest_1721 at 1828.674 kb on + strand at 1828.675 kb on - strand at 1828.809 kb on + strand, within Psest_1722 at 1828.809 kb on + strand, within Psest_1722 at 1828.809 kb on + strand, within Psest_1722 at 1828.809 kb on + strand, within Psest_1722 at 1828.810 kb on - strand, within Psest_1722
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 1,825,833 + Psest_1719 0.44 +0.0 1,826,223 + Psest_1719 0.66 +1.5 1,826,224 - Psest_1719 0.66 +1.1 1,826,224 - Psest_1719 0.66 -0.6 1,826,247 + Psest_1719 0.67 -0.0 1,826,247 + Psest_1719 0.67 +1.1 1,826,248 - Psest_1719 0.67 +0.6 1,826,248 - Psest_1719 0.67 -0.3 1,826,248 - Psest_1719 0.67 +1.8 1,826,248 - Psest_1719 0.67 +1.3 1,826,248 - Psest_1719 0.67 +0.3 1,826,248 - Psest_1719 0.67 +0.6 1,826,248 - Psest_1719 0.67 +1.4 1,826,249 + Psest_1719 0.67 -0.4 1,826,250 - Psest_1719 0.67 -0.4 1,826,250 - Psest_1719 0.67 +3.0 1,827,073 + Psest_1720 0.24 +1.5 1,827,073 + Psest_1720 0.24 -1.7 1,827,073 + Psest_1720 0.24 -1.9 1,827,073 + Psest_1720 0.24 -1.4 1,827,074 - Psest_1720 0.24 -0.6 1,827,136 - Psest_1720 0.30 +0.6 1,827,153 + Psest_1720 0.32 +1.7 1,827,251 - Psest_1720 0.41 +2.1 1,827,425 + Psest_1720 0.59 +0.7 1,827,425 + Psest_1720 0.59 -1.1 1,827,425 + Psest_1720 0.59 +2.8 1,827,426 - Psest_1720 0.59 +2.4 1,827,460 + Psest_1720 0.62 +0.7 1,827,460 + Psest_1720 0.62 -1.7 1,827,460 + Psest_1720 0.62 +2.4 1,827,460 + Psest_1720 0.62 +0.3 1,827,460 + Psest_1720 0.62 +0.4 1,827,460 + Psest_1720 0.62 +0.9 1,827,543 + Psest_1720 0.70 +1.0 1,827,543 + Psest_1720 0.70 +1.0 1,827,543 + Psest_1720 0.70 -1.0 1,827,543 + Psest_1720 0.70 +0.1 1,827,543 + Psest_1720 0.70 +0.4 1,827,543 + Psest_1720 0.70 +1.3 1,827,543 + Psest_1720 0.70 -0.5 1,827,543 + Psest_1720 0.70 -0.3 1,827,544 - Psest_1720 0.70 +1.8 1,827,544 - Psest_1720 0.70 -2.4 1,827,544 - Psest_1720 0.70 -1.3 1,827,544 - Psest_1720 0.70 -0.4 1,827,544 - Psest_1720 0.70 +1.7 1,827,544 - Psest_1720 0.70 +1.5 1,827,544 - Psest_1720 0.70 +0.6 1,827,544 - Psest_1720 0.70 +0.7 1,827,544 - Psest_1720 0.70 +1.1 1,827,544 - Psest_1720 0.70 -2.4 1,827,544 - Psest_1720 0.70 +1.8 1,827,544 - Psest_1720 0.70 +2.6 1,827,544 - Psest_1720 0.70 -0.2 1,827,544 - Psest_1720 0.70 +1.3 1,827,815 + +1.3 1,827,844 + +0.6 1,827,844 + +1.4 1,827,844 + -0.5 1,827,844 + +1.3 1,827,844 + +0.6 1,827,844 + +0.0 1,827,844 + -0.4 1,827,844 + +0.6 1,827,844 + +0.2 1,827,844 + +1.1 1,827,845 - -1.3 1,827,845 - -0.9 1,827,852 + +0.4 1,827,861 + +0.9 1,828,088 + Psest_1721 0.29 +1.4 1,828,088 + Psest_1721 0.29 +1.9 1,828,089 - Psest_1721 0.29 +0.6 1,828,089 - Psest_1721 0.29 -0.1 1,828,140 + Psest_1721 0.35 +1.5 1,828,674 + +2.9 1,828,675 - -0.2 1,828,809 + Psest_1722 0.13 +1.4 1,828,809 + Psest_1722 0.13 +1.4 1,828,809 + Psest_1722 0.13 +1.5 1,828,809 + Psest_1722 0.13 -0.4 1,828,810 - Psest_1722 0.13 +1.2
Or see this region's nucleotide sequence