Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1657 and Psest_1658 are separated by 2 nucleotides Psest_1658 and Psest_1659 overlap by 1 nucleotides Psest_1659 and Psest_1660 are separated by 15 nucleotides
Psest_1657: Psest_1657 - NADH:ubiquinone oxidoreductase, Na(+)-translocating, C subunit, at 1,767,417 to 1,768,211
_1657
Psest_1658: Psest_1658 - NADH:ubiquinone oxidoreductase, Na(+)-translocating, D subunit, at 1,768,214 to 1,768,882
_1658
Psest_1659: Psest_1659 - NADH:ubiquinone oxidoreductase, Na(+)-translocating, E subunit, at 1,768,882 to 1,769,490
_1659
Psest_1660: Psest_1660 - NADH:ubiquinone oxidoreductase, Na(+)-translocating, F subunit, at 1,769,506 to 1,770,729
_1660
Position (kb)
1768
1769
1770 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1768.187 kb on + strand at 1768.487 kb on + strand, within Psest_1658 at 1768.716 kb on + strand, within Psest_1658 at 1768.891 kb on + strand at 1769.059 kb on + strand, within Psest_1659 at 1769.060 kb on - strand, within Psest_1659 at 1769.065 kb on + strand, within Psest_1659 at 1769.140 kb on + strand, within Psest_1659 at 1769.140 kb on + strand, within Psest_1659 at 1769.194 kb on + strand, within Psest_1659 at 1769.209 kb on + strand, within Psest_1659 at 1769.293 kb on + strand, within Psest_1659 at 1769.317 kb on + strand, within Psest_1659 at 1769.389 kb on + strand, within Psest_1659 at 1769.389 kb on + strand, within Psest_1659 at 1769.389 kb on + strand, within Psest_1659 at 1769.389 kb on + strand, within Psest_1659 at 1769.389 kb on + strand, within Psest_1659 at 1769.480 kb on + strand at 1769.488 kb on + strand at 1770.064 kb on + strand, within Psest_1660 at 1770.100 kb on + strand, within Psest_1660 at 1770.244 kb on + strand, within Psest_1660 at 1770.457 kb on + strand, within Psest_1660 at 1770.469 kb on + strand, within Psest_1660 at 1770.469 kb on + strand, within Psest_1660
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 1,768,187 + +0.7 1,768,487 + Psest_1658 0.41 +1.3 1,768,716 + Psest_1658 0.75 +3.6 1,768,891 + +0.0 1,769,059 + Psest_1659 0.29 +0.3 1,769,060 - Psest_1659 0.29 +0.9 1,769,065 + Psest_1659 0.30 +0.3 1,769,140 + Psest_1659 0.42 +0.7 1,769,140 + Psest_1659 0.42 -1.1 1,769,194 + Psest_1659 0.51 +4.0 1,769,209 + Psest_1659 0.54 +2.3 1,769,293 + Psest_1659 0.67 +3.6 1,769,317 + Psest_1659 0.71 -0.7 1,769,389 + Psest_1659 0.83 -0.1 1,769,389 + Psest_1659 0.83 +3.3 1,769,389 + Psest_1659 0.83 +0.3 1,769,389 + Psest_1659 0.83 +1.6 1,769,389 + Psest_1659 0.83 +2.3 1,769,480 + +0.7 1,769,488 + +1.3 1,770,064 + Psest_1660 0.46 -2.3 1,770,100 + Psest_1660 0.49 -1.1 1,770,244 + Psest_1660 0.60 +0.7 1,770,457 + Psest_1660 0.78 -1.7 1,770,469 + Psest_1660 0.79 +2.2 1,770,469 + Psest_1660 0.79 -1.5
Or see this region's nucleotide sequence