Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1247 and Psest_1248 are separated by 154 nucleotides Psest_1248 and Psest_1249 overlap by 1 nucleotides Psest_1249 and Psest_1250 are separated by 159 nucleotides Psest_1250 and Psest_1251 are separated by 31 nucleotides
Psest_1247: Psest_1247 - Archaeal fructose-1,6-bisphosphatase and related enzymes of inositol monophosphatase family, at 1,355,116 to 1,355,931
_1247
Psest_1248: Psest_1248 - RNA methyltransferase, TrmH family, group 1, at 1,356,086 to 1,356,862
_1248
Psest_1249: Psest_1249 - serine O-acetyltransferase, at 1,356,862 to 1,357,638
_1249
Psest_1250: Psest_1250 - iron-sulfur cluster assembly transcription factor IscR, at 1,357,798 to 1,358,289
_1250
Psest_1251: Psest_1251 - cysteine desulfurase IscS, at 1,358,321 to 1,359,535
_1251
Position (kb)
1356
1357
1358 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1355.993 kb on + strand at 1355.993 kb on + strand at 1355.994 kb on - strand at 1355.994 kb on - strand at 1356.051 kb on + strand at 1356.052 kb on - strand at 1356.307 kb on + strand, within Psest_1248 at 1356.307 kb on + strand, within Psest_1248 at 1356.307 kb on + strand, within Psest_1248 at 1356.308 kb on - strand, within Psest_1248 at 1356.308 kb on - strand, within Psest_1248 at 1356.308 kb on - strand, within Psest_1248 at 1356.479 kb on + strand, within Psest_1248 at 1356.490 kb on + strand, within Psest_1248 at 1356.491 kb on - strand, within Psest_1248 at 1356.551 kb on + strand, within Psest_1248 at 1356.551 kb on + strand, within Psest_1248 at 1356.551 kb on + strand, within Psest_1248 at 1356.551 kb on + strand, within Psest_1248 at 1356.552 kb on - strand, within Psest_1248 at 1356.552 kb on - strand, within Psest_1248 at 1356.552 kb on - strand, within Psest_1248 at 1356.552 kb on - strand, within Psest_1248 at 1356.552 kb on - strand, within Psest_1248 at 1356.665 kb on + strand, within Psest_1248 at 1356.666 kb on - strand, within Psest_1248 at 1356.666 kb on - strand, within Psest_1248 at 1356.666 kb on - strand, within Psest_1248 at 1356.698 kb on - strand, within Psest_1248 at 1357.160 kb on + strand, within Psest_1249 at 1357.160 kb on + strand, within Psest_1249 at 1357.160 kb on + strand, within Psest_1249 at 1357.160 kb on + strand, within Psest_1249 at 1357.160 kb on + strand, within Psest_1249 at 1357.160 kb on + strand, within Psest_1249 at 1357.160 kb on + strand, within Psest_1249 at 1357.160 kb on + strand, within Psest_1249 at 1357.161 kb on - strand, within Psest_1249 at 1357.161 kb on - strand, within Psest_1249 at 1357.161 kb on - strand, within Psest_1249 at 1357.161 kb on - strand, within Psest_1249 at 1357.161 kb on - strand, within Psest_1249 at 1357.161 kb on - strand, within Psest_1249 at 1357.161 kb on - strand, within Psest_1249 at 1357.161 kb on - strand, within Psest_1249 at 1357.161 kb on - strand, within Psest_1249 at 1357.161 kb on - strand, within Psest_1249 at 1357.161 kb on - strand, within Psest_1249 at 1357.161 kb on - strand, within Psest_1249 at 1357.161 kb on - strand, within Psest_1249 at 1357.161 kb on - strand, within Psest_1249 at 1357.162 kb on + strand, within Psest_1249 at 1357.163 kb on - strand, within Psest_1249 at 1357.232 kb on + strand, within Psest_1249 at 1357.450 kb on - strand, within Psest_1249 at 1357.450 kb on - strand, within Psest_1249 at 1357.525 kb on - strand, within Psest_1249 at 1357.525 kb on - strand, within Psest_1249 at 1357.663 kb on + strand at 1357.853 kb on + strand, within Psest_1250 at 1357.853 kb on + strand, within Psest_1250 at 1358.267 kb on + strand at 1358.267 kb on + strand at 1358.267 kb on + strand at 1358.267 kb on + strand at 1358.267 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 1,355,993 + +3.1 1,355,993 + -1.2 1,355,994 - -0.3 1,355,994 - -1.2 1,356,051 + -0.1 1,356,052 - +0.1 1,356,307 + Psest_1248 0.28 -0.8 1,356,307 + Psest_1248 0.28 +0.8 1,356,307 + Psest_1248 0.28 -2.4 1,356,308 - Psest_1248 0.29 -0.8 1,356,308 - Psest_1248 0.29 +0.2 1,356,308 - Psest_1248 0.29 -0.2 1,356,479 + Psest_1248 0.51 -2.8 1,356,490 + Psest_1248 0.52 -1.7 1,356,491 - Psest_1248 0.52 +0.3 1,356,551 + Psest_1248 0.60 +0.7 1,356,551 + Psest_1248 0.60 +0.8 1,356,551 + Psest_1248 0.60 -1.2 1,356,551 + Psest_1248 0.60 +0.1 1,356,552 - Psest_1248 0.60 +0.8 1,356,552 - Psest_1248 0.60 +0.5 1,356,552 - Psest_1248 0.60 +0.1 1,356,552 - Psest_1248 0.60 -1.1 1,356,552 - Psest_1248 0.60 +3.6 1,356,665 + Psest_1248 0.75 -0.2 1,356,666 - Psest_1248 0.75 +2.6 1,356,666 - Psest_1248 0.75 +0.9 1,356,666 - Psest_1248 0.75 -0.8 1,356,698 - Psest_1248 0.79 +0.2 1,357,160 + Psest_1249 0.38 -1.5 1,357,160 + Psest_1249 0.38 +2.6 1,357,160 + Psest_1249 0.38 +0.9 1,357,160 + Psest_1249 0.38 +1.0 1,357,160 + Psest_1249 0.38 -0.4 1,357,160 + Psest_1249 0.38 +1.5 1,357,160 + Psest_1249 0.38 +0.6 1,357,160 + Psest_1249 0.38 +1.8 1,357,161 - Psest_1249 0.38 +0.3 1,357,161 - Psest_1249 0.38 +0.7 1,357,161 - Psest_1249 0.38 +3.3 1,357,161 - Psest_1249 0.38 +0.2 1,357,161 - Psest_1249 0.38 +2.9 1,357,161 - Psest_1249 0.38 +0.9 1,357,161 - Psest_1249 0.38 -1.5 1,357,161 - Psest_1249 0.38 +0.9 1,357,161 - Psest_1249 0.38 -1.7 1,357,161 - Psest_1249 0.38 +1.8 1,357,161 - Psest_1249 0.38 +2.2 1,357,161 - Psest_1249 0.38 +1.6 1,357,161 - Psest_1249 0.38 -0.1 1,357,161 - Psest_1249 0.38 +0.1 1,357,162 + Psest_1249 0.39 +2.7 1,357,163 - Psest_1249 0.39 +1.5 1,357,232 + Psest_1249 0.48 +0.6 1,357,450 - Psest_1249 0.76 +2.2 1,357,450 - Psest_1249 0.76 +2.5 1,357,525 - Psest_1249 0.85 +2.5 1,357,525 - Psest_1249 0.85 +3.3 1,357,663 + -1.0 1,357,853 + Psest_1250 0.11 +1.8 1,357,853 + Psest_1250 0.11 -1.2 1,358,267 + -0.1 1,358,267 + +1.3 1,358,267 + +0.1 1,358,267 + -0.2 1,358,267 + -1.0
Or see this region's nucleotide sequence