Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1249

Experiment: Neomycin 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1247 and Psest_1248 are separated by 154 nucleotidesPsest_1248 and Psest_1249 overlap by 1 nucleotidesPsest_1249 and Psest_1250 are separated by 159 nucleotidesPsest_1250 and Psest_1251 are separated by 31 nucleotides Psest_1247: Psest_1247 - Archaeal fructose-1,6-bisphosphatase and related enzymes of inositol monophosphatase family, at 1,355,116 to 1,355,931 _1247 Psest_1248: Psest_1248 - RNA methyltransferase, TrmH family, group 1, at 1,356,086 to 1,356,862 _1248 Psest_1249: Psest_1249 - serine O-acetyltransferase, at 1,356,862 to 1,357,638 _1249 Psest_1250: Psest_1250 - iron-sulfur cluster assembly transcription factor IscR, at 1,357,798 to 1,358,289 _1250 Psest_1251: Psest_1251 - cysteine desulfurase IscS, at 1,358,321 to 1,359,535 _1251 Position (kb) 1356 1357 1358Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1355.993 kb on + strandat 1355.993 kb on + strandat 1355.994 kb on - strandat 1355.994 kb on - strandat 1356.051 kb on + strandat 1356.052 kb on - strandat 1356.307 kb on + strand, within Psest_1248at 1356.307 kb on + strand, within Psest_1248at 1356.307 kb on + strand, within Psest_1248at 1356.308 kb on - strand, within Psest_1248at 1356.308 kb on - strand, within Psest_1248at 1356.308 kb on - strand, within Psest_1248at 1356.479 kb on + strand, within Psest_1248at 1356.490 kb on + strand, within Psest_1248at 1356.491 kb on - strand, within Psest_1248at 1356.551 kb on + strand, within Psest_1248at 1356.551 kb on + strand, within Psest_1248at 1356.551 kb on + strand, within Psest_1248at 1356.551 kb on + strand, within Psest_1248at 1356.552 kb on - strand, within Psest_1248at 1356.552 kb on - strand, within Psest_1248at 1356.552 kb on - strand, within Psest_1248at 1356.552 kb on - strand, within Psest_1248at 1356.552 kb on - strand, within Psest_1248at 1356.665 kb on + strand, within Psest_1248at 1356.666 kb on - strand, within Psest_1248at 1356.666 kb on - strand, within Psest_1248at 1356.666 kb on - strand, within Psest_1248at 1356.698 kb on - strand, within Psest_1248at 1357.160 kb on + strand, within Psest_1249at 1357.160 kb on + strand, within Psest_1249at 1357.160 kb on + strand, within Psest_1249at 1357.160 kb on + strand, within Psest_1249at 1357.160 kb on + strand, within Psest_1249at 1357.160 kb on + strand, within Psest_1249at 1357.160 kb on + strand, within Psest_1249at 1357.160 kb on + strand, within Psest_1249at 1357.161 kb on - strand, within Psest_1249at 1357.161 kb on - strand, within Psest_1249at 1357.161 kb on - strand, within Psest_1249at 1357.161 kb on - strand, within Psest_1249at 1357.161 kb on - strand, within Psest_1249at 1357.161 kb on - strand, within Psest_1249at 1357.161 kb on - strand, within Psest_1249at 1357.161 kb on - strand, within Psest_1249at 1357.161 kb on - strand, within Psest_1249at 1357.161 kb on - strand, within Psest_1249at 1357.161 kb on - strand, within Psest_1249at 1357.161 kb on - strand, within Psest_1249at 1357.161 kb on - strand, within Psest_1249at 1357.161 kb on - strand, within Psest_1249at 1357.162 kb on + strand, within Psest_1249at 1357.163 kb on - strand, within Psest_1249at 1357.232 kb on + strand, within Psest_1249at 1357.450 kb on - strand, within Psest_1249at 1357.450 kb on - strand, within Psest_1249at 1357.525 kb on - strand, within Psest_1249at 1357.525 kb on - strand, within Psest_1249at 1357.663 kb on + strandat 1357.853 kb on + strand, within Psest_1250at 1357.853 kb on + strand, within Psest_1250at 1358.267 kb on + strandat 1358.267 kb on + strandat 1358.267 kb on + strandat 1358.267 kb on + strandat 1358.267 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml
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1,355,993 + +3.1
1,355,993 + -1.2
1,355,994 - -0.3
1,355,994 - -1.2
1,356,051 + -0.1
1,356,052 - +0.1
1,356,307 + Psest_1248 0.28 -0.8
1,356,307 + Psest_1248 0.28 +0.8
1,356,307 + Psest_1248 0.28 -2.4
1,356,308 - Psest_1248 0.29 -0.8
1,356,308 - Psest_1248 0.29 +0.2
1,356,308 - Psest_1248 0.29 -0.2
1,356,479 + Psest_1248 0.51 -2.8
1,356,490 + Psest_1248 0.52 -1.7
1,356,491 - Psest_1248 0.52 +0.3
1,356,551 + Psest_1248 0.60 +0.7
1,356,551 + Psest_1248 0.60 +0.8
1,356,551 + Psest_1248 0.60 -1.2
1,356,551 + Psest_1248 0.60 +0.1
1,356,552 - Psest_1248 0.60 +0.8
1,356,552 - Psest_1248 0.60 +0.5
1,356,552 - Psest_1248 0.60 +0.1
1,356,552 - Psest_1248 0.60 -1.1
1,356,552 - Psest_1248 0.60 +3.6
1,356,665 + Psest_1248 0.75 -0.2
1,356,666 - Psest_1248 0.75 +2.6
1,356,666 - Psest_1248 0.75 +0.9
1,356,666 - Psest_1248 0.75 -0.8
1,356,698 - Psest_1248 0.79 +0.2
1,357,160 + Psest_1249 0.38 -1.5
1,357,160 + Psest_1249 0.38 +2.6
1,357,160 + Psest_1249 0.38 +0.9
1,357,160 + Psest_1249 0.38 +1.0
1,357,160 + Psest_1249 0.38 -0.4
1,357,160 + Psest_1249 0.38 +1.5
1,357,160 + Psest_1249 0.38 +0.6
1,357,160 + Psest_1249 0.38 +1.8
1,357,161 - Psest_1249 0.38 +0.3
1,357,161 - Psest_1249 0.38 +0.7
1,357,161 - Psest_1249 0.38 +3.3
1,357,161 - Psest_1249 0.38 +0.2
1,357,161 - Psest_1249 0.38 +2.9
1,357,161 - Psest_1249 0.38 +0.9
1,357,161 - Psest_1249 0.38 -1.5
1,357,161 - Psest_1249 0.38 +0.9
1,357,161 - Psest_1249 0.38 -1.7
1,357,161 - Psest_1249 0.38 +1.8
1,357,161 - Psest_1249 0.38 +2.2
1,357,161 - Psest_1249 0.38 +1.6
1,357,161 - Psest_1249 0.38 -0.1
1,357,161 - Psest_1249 0.38 +0.1
1,357,162 + Psest_1249 0.39 +2.7
1,357,163 - Psest_1249 0.39 +1.5
1,357,232 + Psest_1249 0.48 +0.6
1,357,450 - Psest_1249 0.76 +2.2
1,357,450 - Psest_1249 0.76 +2.5
1,357,525 - Psest_1249 0.85 +2.5
1,357,525 - Psest_1249 0.85 +3.3
1,357,663 + -1.0
1,357,853 + Psest_1250 0.11 +1.8
1,357,853 + Psest_1250 0.11 -1.2
1,358,267 + -0.1
1,358,267 + +1.3
1,358,267 + +0.1
1,358,267 + -0.2
1,358,267 + -1.0

Or see this region's nucleotide sequence