Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0741 and Psest_0742 are separated by 3 nucleotides Psest_0742 and Psest_0743 are separated by 100 nucleotides Psest_0743 and Psest_0744 are separated by 360 nucleotides
Psest_0741: Psest_0741 - drug resistance transporter, Bcr/CflA subfamily, at 814,286 to 815,458
_0741
Psest_0742: Psest_0742 - hypothetical protein, at 815,462 to 815,626
_0742
Psest_0743: Psest_0743 - adenosine deaminase, at 815,727 to 816,776
_0743
Psest_0744: Psest_0744 - Uncharacterized conserved protein, at 817,137 to 817,724
_0744
Position (kb) 815
816
817 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 814.832 kb on + strand, within Psest_0741 at 814.833 kb on - strand, within Psest_0741 at 814.880 kb on + strand, within Psest_0741 at 814.881 kb on - strand, within Psest_0741 at 814.948 kb on + strand, within Psest_0741 at 814.958 kb on + strand, within Psest_0741 at 814.958 kb on + strand, within Psest_0741 at 814.959 kb on - strand, within Psest_0741 at 814.959 kb on - strand, within Psest_0741 at 814.959 kb on - strand, within Psest_0741 at 814.994 kb on + strand, within Psest_0741 at 814.994 kb on + strand, within Psest_0741 at 815.573 kb on - strand, within Psest_0742 at 815.595 kb on + strand, within Psest_0742 at 815.640 kb on + strand at 815.642 kb on + strand at 815.830 kb on + strand at 815.842 kb on + strand, within Psest_0743 at 815.843 kb on - strand, within Psest_0743 at 816.071 kb on - strand, within Psest_0743 at 816.071 kb on - strand, within Psest_0743 at 816.455 kb on - strand, within Psest_0743 at 816.455 kb on - strand, within Psest_0743 at 816.455 kb on - strand, within Psest_0743 at 816.474 kb on + strand, within Psest_0743 at 816.475 kb on - strand, within Psest_0743 at 816.493 kb on + strand, within Psest_0743 at 816.494 kb on - strand, within Psest_0743 at 816.494 kb on - strand, within Psest_0743 at 816.496 kb on + strand, within Psest_0743 at 816.496 kb on + strand, within Psest_0743 at 816.736 kb on + strand at 816.736 kb on + strand at 816.737 kb on - strand at 816.763 kb on + strand at 816.763 kb on + strand at 816.784 kb on + strand at 816.784 kb on + strand at 816.784 kb on + strand at 816.784 kb on + strand at 816.785 kb on - strand at 816.785 kb on - strand at 816.785 kb on - strand at 816.785 kb on - strand at 816.882 kb on + strand at 816.883 kb on - strand at 817.086 kb on + strand at 817.086 kb on + strand at 817.086 kb on + strand at 817.087 kb on - strand at 817.116 kb on + strand at 817.117 kb on - strand at 817.117 kb on - strand at 817.117 kb on - strand at 817.138 kb on + strand at 817.138 kb on + strand at 817.139 kb on - strand at 817.139 kb on - strand at 817.175 kb on + strand at 817.584 kb on - strand, within Psest_0744 at 817.750 kb on + strand at 817.767 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 814,832 + Psest_0741 0.47 -1.5 814,833 - Psest_0741 0.47 +0.1 814,880 + Psest_0741 0.51 +3.4 814,881 - Psest_0741 0.51 -1.0 814,948 + Psest_0741 0.56 +0.8 814,958 + Psest_0741 0.57 +1.2 814,958 + Psest_0741 0.57 -0.5 814,959 - Psest_0741 0.57 +0.7 814,959 - Psest_0741 0.57 +0.3 814,959 - Psest_0741 0.57 -1.2 814,994 + Psest_0741 0.60 +0.5 814,994 + Psest_0741 0.60 +0.2 815,573 - Psest_0742 0.67 +0.2 815,595 + Psest_0742 0.81 -1.7 815,640 + -0.0 815,642 + -0.1 815,830 + -1.4 815,842 + Psest_0743 0.11 -0.1 815,843 - Psest_0743 0.11 +0.2 816,071 - Psest_0743 0.33 +0.1 816,071 - Psest_0743 0.33 -1.8 816,455 - Psest_0743 0.69 +0.0 816,455 - Psest_0743 0.69 -1.5 816,455 - Psest_0743 0.69 -0.9 816,474 + Psest_0743 0.71 +0.2 816,475 - Psest_0743 0.71 -2.8 816,493 + Psest_0743 0.73 -3.0 816,494 - Psest_0743 0.73 -1.0 816,494 - Psest_0743 0.73 -0.9 816,496 + Psest_0743 0.73 -1.0 816,496 + Psest_0743 0.73 -1.4 816,736 + +1.0 816,736 + -1.3 816,737 - +1.6 816,763 + -1.7 816,763 + -1.2 816,784 + -0.4 816,784 + -0.7 816,784 + -0.3 816,784 + -1.8 816,785 - -0.1 816,785 - +0.2 816,785 - +1.1 816,785 - +0.4 816,882 + -0.1 816,883 - -0.6 817,086 + +0.4 817,086 + -1.4 817,086 + +1.0 817,087 - +0.0 817,116 + -1.4 817,117 - -0.4 817,117 - -1.0 817,117 - +1.0 817,138 + +0.2 817,138 + -1.2 817,139 - -0.1 817,139 - +0.3 817,175 + +0.1 817,584 - Psest_0744 0.76 -0.0 817,750 + +0.3 817,767 - +1.2
Or see this region's nucleotide sequence