Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_4000 and Psest_4001 are separated by 39 nucleotides Psest_4001 and Psest_4002 are separated by 129 nucleotides
Psest_4000: Psest_4000 - amino-acid N-acetyltransferase, at 4,171,605 to 4,172,903
_4000
Psest_4001: Psest_4001 - acetylornithine deacetylase (ArgE), at 4,172,943 to 4,174,103
_4001
Psest_4002: Psest_4002 - Phosphate/sulphate permeases, at 4,174,233 to 4,175,498
_4002
Position (kb)
4172
4173
4174
4175 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4172.894 kb on - strand at 4172.894 kb on - strand at 4172.940 kb on + strand at 4172.940 kb on + strand at 4172.940 kb on + strand at 4172.974 kb on + strand at 4172.975 kb on - strand at 4172.975 kb on - strand at 4172.975 kb on - strand at 4173.035 kb on - strand at 4173.109 kb on + strand, within Psest_4001 at 4173.110 kb on - strand, within Psest_4001 at 4173.359 kb on + strand, within Psest_4001 at 4173.359 kb on + strand, within Psest_4001 at 4173.359 kb on + strand, within Psest_4001 at 4173.360 kb on - strand, within Psest_4001 at 4173.360 kb on - strand, within Psest_4001 at 4173.360 kb on - strand, within Psest_4001 at 4173.414 kb on + strand, within Psest_4001 at 4173.415 kb on - strand, within Psest_4001 at 4173.437 kb on - strand, within Psest_4001 at 4173.558 kb on - strand, within Psest_4001 at 4173.913 kb on + strand, within Psest_4001 at 4174.109 kb on - strand at 4174.109 kb on - strand at 4174.109 kb on - strand at 4174.127 kb on + strand at 4174.215 kb on + strand at 4174.215 kb on + strand at 4174.215 kb on + strand at 4174.215 kb on + strand at 4174.216 kb on - strand at 4174.942 kb on + strand, within Psest_4002
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 4,172,894 - -0.0 4,172,894 - -0.7 4,172,940 + -0.4 4,172,940 + -0.6 4,172,940 + -2.3 4,172,974 + +2.1 4,172,975 - -0.4 4,172,975 - -1.2 4,172,975 - -1.9 4,173,035 - -0.4 4,173,109 + Psest_4001 0.14 -1.2 4,173,110 - Psest_4001 0.14 -1.0 4,173,359 + Psest_4001 0.36 -1.1 4,173,359 + Psest_4001 0.36 -2.6 4,173,359 + Psest_4001 0.36 +1.3 4,173,360 - Psest_4001 0.36 -1.7 4,173,360 - Psest_4001 0.36 -2.8 4,173,360 - Psest_4001 0.36 -0.1 4,173,414 + Psest_4001 0.41 +0.7 4,173,415 - Psest_4001 0.41 -1.5 4,173,437 - Psest_4001 0.43 -0.5 4,173,558 - Psest_4001 0.53 -0.3 4,173,913 + Psest_4001 0.84 -1.4 4,174,109 - -0.7 4,174,109 - +2.0 4,174,109 - -0.2 4,174,127 + +1.6 4,174,215 + +2.5 4,174,215 + +1.5 4,174,215 + +2.0 4,174,215 + -0.2 4,174,216 - -0.1 4,174,942 + Psest_4002 0.56 +1.4
Or see this region's nucleotide sequence